Analysis of promoter activity reveals that GmFTL2 expression differs from that of the known Flowering Locus T genes in soybean

作     者：Limin Liu Xiaomei Zhang Fulu Chen Asia Adam Elzamzami Mahi Xiaoxia Wu Qingshan Chen Yong-Fu Fu 

作者机构：College of AgricultureNortheast Agricultural University MOA Key Lab of Soybean Biology (Beijing) National Key Facility for Crop Gene Resources and Genetic Improvement Institute of Crop ScienceChinese Academy of Agricultural Sciences 

出 版 物：《The Crop Journal》 (作物学报（英文版）)

年 卷 期：2017年第5卷第5期

页      面：438-447页

核心收录：

学科分类：09[农学] 0901[农学-作物学] 

基　　金：supported by the National Key Research and Development Program of China (2016YFD0101005) the National Natural Science Foundation of China (31371703 and 31570289) 

主　　题：Flowering Locus T Cotyledon Microgametophyte Embryo Root tip 

摘      要：Regulation of flowering is one of the key issues in crop yield. The Floiuering Loews T(FT) gene is a well-known florigen, which integrates various signals from multiple flowering-regulation pathways to initiate flowering. We previously reported that there are at least six FT genes(GmFTLl-6) in soybean displaying flowering activity. However, the individual functions of genes GmFTLl-6 remain to be identified. In this study, we cloned the GmFTL2 promoter(GmFTLpro) from soybean(Glycine max) cultivar Tianlong 1 and analyzed its motifs bioinformatically and its expression patterns using both a transgenic approach and quantitative RT-PCR(qRT-PCR). In GmFTLpro::GUS transgenic lines, GUS signals were enriched in cotyledons, hypocotyledons, pollen, embryos, and root tips in a photoperiod-independent manner. qRT-PCR confirmed the GUS reporter results. Our results suggest that GmFTL2 expression is regulated by developmental and tissue-specific clues and plays roles in seedling establishment and the development of micro game tophytes, embryos, and roots.