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Impacts of oxygen deficiency on Embryo life-history traits of migratory locust Locusta migratoria from low and high altitudes

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Insect Science 2023年第3期30卷 867-879页

作者： Xianliang Huang Qianli Du Lijing Wang Bing Chen School of Life Science Institutes of Life Science and Green DevelopmentHebei UniversityBaodingHebei ProvinceChina Kenli Municipal Bureau of Agriculture and Rural Affairs DongyingShandong ProvinceChina

Hypoxia challenges aerobic organisms in numerous environments,and hypoxic conditions may become more severe under future climate-change scenarios.The impact of hypoxia on the development of terrestrial insect Embryos is not well understood.Here,to address this gap,Embryonic life-history traits of migratory locust Locusta migratoria from low-altitude and high-altitude regions were compared under 2 oxygen levels:normoxia(i.e.,21 kPa oxygen partial pressure and mild hypoxia(i.e.,10 kPa oxygen partial pressure).Our results demonstrated that,whether reared under normoxia or mild hypoxia,L.migratoria from high-altitude populations had longer developmental times,reduced weight,and lower mean relative growth rate as compared with those from low-altitude populations.When transferred from normoxia to mild hypoxia,nearly all the tested lifehistory traits presented significant negative changes in the low-altitude populations,but not in the high-altitude populations.The factor'strain'alone explained 18.26%-54.59%of the total variation for traits,suggesting that the phenotypic differences between L.migratoria populations from the 2 altitudes could be driven by genetic variation.Significant genetic correlations were found between life-history traits,and most of these showed differentiation between the 2 altitudinal gradients.G-matrix comparisons showed significant structural differences between L.migratoria from the 2 regions,as well as several negative covariances(i.e.,trade-offs)between traits in the low-altitude populations.Overall,our study provides clear evidence that evolutionary divergence of Embryonic traits between L.migratoria populations from different altitudes has occurred.

关键词： Embryo life-history traits Locusta migratoria oxygen deficiency population divergence

Homeobox protein A1-like and DNA methylation regulate Embryo-specific Zinc finger protein 615 gene expression and Embryonic development in the silkworm Bombyx mori

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Insect Science 2023年第4期30卷 1063-1080页

作者： Yu-Lin Tian Tong-Yu Fu Qi-En Zhong Yi-Guang Lin Si-Chun Zheng Guan-Feng Xu Guangdong Provincial Key Laboratory of Insect Developmental Biology and Applied Technology Guangzhou Key Laboratory of Insect Development Regulation and Applied ResearchInstitute of Insect Science and TechnologySchool of Life SciencesSouth China Normal UniversityGuangzhouChina

DNA methylation and transcription factors play roles in gene expression and animal development.In insects,DNA methylation modifies gene bodies,but how DNA methylation and transcription factors regulate gene expression is unclear.In this study,we investigated the mechanism that regulates the expression of Bombyx mori Zinc finger pro-tein 615(ZnF 615),which is a downstream gene of DNA methyltransferase 1(Dnmt1),and its effects on the regulation of Embryonic development.By progressively truncating the ZnF 615 promoter,it was found that the-223 and-190 nt region,which contains homeobox(Hox)protein cis-regulatory elements(CREs),had the greatest impact on the transcription of ZnF 615.RNA interference(RNAi)-mediated knockdown and overexpres-sion of Hox family genes showed that Hox A1-like can enhance the messenger RNA level of ZnF 615.Further studies showed that Hox A1-like regulates ZnF 615 expression by directly binding to the-223 and-190 nt region of its promoter.Simultaneous RNAi-mediated knockdown or overexpression of Hox A1-like and Dnmtl significantly inhibited or enhanced the regulatory effect of either gene alone on ZnF 615 expression,suggesting that both DNA methylation of gene bodies and binding of transcription factors to pro-moters are essential for gene expression.RNAi-mediated knockdown of Hox A1-like and Dnmtl showed that the Embryonic development was retarded and the hatching rate was decreased.Taken together,these data suggest that Hox A1-like and DNA methylation en-hance the expression of ZnF 615,thereby affecting the development of B.mori Embryos.

关键词： Bombyx mori DNA methylation Embryo gene expression Hox Al-like ovary

Homology-based repair induced by CRISPR-Cas nucleases in mammalian Embryo genome editing

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Protein & Cell 2022年第5期13卷 316-335页

作者： Xiya Zhang Tao Li Jianping Ou Junjiu Huang Puping Liang Center for Reproductive Medicine the Third Affiliated Hospital of Sun Yat-sen UniversitySun Yat-sen UniversityGuangzhou 510630China MOE Key Laboratory of Gene Function and Regulation State Key Laboratory of BiocontrolSchool of Life SciencesSun Yat-sen UniversityGuangzhou 510275China Key Laboratory of Reproductive Medicine of Guangdong Province the First Affiliated Hospital and School of Life SciencesSun Yat-sen UniversityGuangzhou 510275China

Recent advances in genome editing,especially CRISPR-Cas nucleases,have revolutionized both laboratory research and clinical therapeutics.CRISPR-Cas nucleases,together with the DNA damage repair pathway in cells,enable both genetic diversification by classical non-homologous end joining(c-NHEJ)and precise genome modification by homology-based repair(HBR).Genome editing in zygotes is a convenient way to edit the germline,paving the way for animal disease model generation,as well as human Embryo genome editing therapy for some life-threatening and incurable diseases.HBR efficiency is highly dependent on the DNA donor that is utilized as a repair template.Here,we review recent progress in improving CRISPR-Cas nuclease-induced HBR in mammalian Embryos by designing a suitable DNA donor.Moreover,we want to provide a guide for producing animal disease models and correcting genetic mutations through CRISPR-Cas nuclease-induced HBR in mammalian Embryos.Finally,we discuss recent developments in precise genome-modification technology based on the CRISPR-Cas system.

关键词： homology-based repair(HBR) genome editing disease modeling Embryo precision medicine

The m6A methylation regulates gonadal sex differentiation in chicken Embryo

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Journal of Animal Science and Biotechnology 2022年第6期13卷 1657-1670页

作者： Jianbo Li Xiuan Zhang Xiqiong Wang Congjiao Sun Jiangxia Zheng Junying Li Guoqiang Yi Ning Yang National Engineering Laboratory for Animal Breeding and Key Laboratory of Animal Genetics Breeding and ReproductionMinistry of Agriculture and Rural AffairsChina Agricultural UniversityBeijingChina Shenzhen Branch Guangdong Laboratory of Lingnan Modern AgricultureGenome Analysis Laboratory of the Ministry of Agriculture and Rural AffairsAgricultural Genomics Institute at ShenzhenChinese Academy of Agricultural SciencesShenzhenChina

Background:As a ubiquitous reversible epigenetic RNA modification,N6-methyladenosine(m6A)plays crucial regulatory roles in multiple biological pathways.However,its functional mechanisms in sex determination and differentiation during gonadal development of chicken Embryos are not clear.Therefore,we established a transcriptome-wide m6A map in the female and male chicken left gonads of Embryonic day 7(E7)by methylated RNA immunoprecipitation sequencing(MeRIP-seq)to offer insight into the landscape of m6A methylation and investigate the post-transcriptional modification underlying gonadal differentiation.Results:The chicken Embryonic gonadal transcriptome was extensively methylated.We found 15,191 and 16,111 m6A peaks in the female and male left gonads,respectively,which were mainly enriched in the coding sequence(CDS)and stop codon.Among these m6A peaks,we identified that 1013 and 751 were hypermethylated in females and males,respectively.These differential peaks covered 281 and 327 genes,such as BMP2,SMAD2,SOX9 and CYP19A1,which were primarily associated with development,morphogenesis and sex differentiation by functional enrichment.Further analysis revealed that the m6A methylation level was positively correlated with gene expression abundance.Furthermore,we found that YTHDC2 could regulate the expression of sex-related genes,especially HEMGN and SOX9,in male mesonephros/gonad mingle cells,which was verified by in vitro experiments,suggesting a regulatory role of m6A methylation in chicken gonad differentiation.Conclusions:This work provided a comprehensive m6A methylation profile of chicken Embryonic gonads and revealed YTHDC2 as a key regulator responsible for sex differentiation.Our results contribute to a better understanding of epigenetic factors involved in chicken sex determination and differentiation and to promoting the future development of sex manipulation in poultry industry.

关键词： Chicken Embryo Gonads m6A YTHDC2

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OS1 functions in the allocation of nutrients between the endosperm and Embryo in maize seeds

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Journal of Integrative Plant Biology 2019年第6期61卷 706-727页

作者： Weibin Song Jinjie Zhu Haiming Zhao Yingnan Li Jiangtao Liu Xiangbo Zhang Liangliang Huang Jinsheng Lai State Key Laboratory of Agrobiotechnology and National Maize Improvement Center Department of Plant Genetics and Breeding ChinaAgricultural University

Uncovering the genetic basis of seed develop-ment will provide useful tools for improving both crop yield and nutritional value, However, the genetic regulatory networks of maize (Zea mays) seed development remain largely unknown. The maize opaque endosperm and small germ 1 (osl) mutant has opaque endosperm and a small Embryo,Here, we cloned OSi1 and show that it encodes a putative transcription factor containing an RWP-RK domain-Transcriptional analysis indicated that OSi1 expression is elevated in early endosperm development, especially in the basal endosperm transfer layer (BETL), conducting zone (CZ), and central starch endospcrm (CSE) cells. RNA sequencing (RNA-Seq) analysis of the osi mutant revealed sharp downregulation of certain genes in specific cell types, including ZmMRP-1 and Megl in BETL cells and a majority of zein-and starch-related genes in CSE cells. Using a haploid induction system, we show that wild-type endosperm could rescue the smaller size of osi Embryo, which suggests that nutrients are allocated by tho wild type endosperm. Therefore, our dsta imply that the network regulated by 051 accomplishes a key step in nutrient allocation between endosperm and Embryo within maize seeds. Identification of this network will help uncover the mechanisms regulating the nutritional balance between endosperm and Embryo.

关键词： 0S1 functions the allocation of nutrients between the endosperm and Embryo in maize seeds^FA

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Impact of gamete health on fertilization and Embryo development: An overview

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Asian pacific Journal of Reproduction 2022年第5期11卷 201-207页

作者： Chorya Jaypalsinh B Sutaria Tarunkumar V Chaudhari Ravjibhai K Chaudhari Chandrakant F Department of Gynaecology and Obstetrics College of Veterinary Science and Animal HusbandryKamdhenu UniversitySardarkrushinagarGujaratIndia

A genetically and functionally proficient gamete is essential for normal fertilization and Embryonic development.Any change in gamete health affects fertilization and subsequent events,including Embryonic development,implantation,and successful pregnancy.This present review focuses on the role of gamete health on fertilization and Embryo development.Several conventional and advanced methods are used to evaluate the morphology and functions of gametes.The abnormal spermatozoa adversely affect fertilization events,which results in reduced cleavage/blastocyst/implantation and pregnancy rate during assisted reproductive techniques.Poor oocyte quality is also one of the reasons for infertility,although the oocyte has an innate capacity to repair a certain amount of abnormality of both oocyte and spermatozoa.Therefore,oocyte health carries more responsibilities during fertilization events.The gamete,either spermatozoa or oocyte,should have optimum morphological and functional health to fertilize and develop a competent Embryo successfully.Thus,it is of prime importance to consider the gamete health parameters while dealing with infertility.

关键词： Embryo Fertilization Gamete Oocyte Sperm

Advances in research into gamete and Embryo-fetal origins of adult diseases

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Science China(Life Sciences) 2019年第3期62卷 360-368页

作者： Kexin Zou Guolian Ding Hefeng Huang The International Peace Maternity and Child Health Hospital School of Medicine Shanghai Jiao Tong University Institute of Embryo-Fetal Original Adult Disease School of Medicine Shanghai Jiao Tong University

The fetal and infant origins of adult disease hypothesis proposed that the roots of adult chronic disease lie in the effects of adverse environments in fetal life and early infancy. In addition to the fetal period, fertilization and early Embryonic stages, the critical time windows of epigenetic reprogramming, rapid cell differentiation and organogenesis, are the most sensitive stages to environmental disturbances. Compared with Embryo and fetal development, gametogenesis and maturation take decades and are more vulnerable to potential damage for a longer exposure period. Therefore, we should shift the focus of adult disease occurrence and pathogenesis further back to gametogenesis and Embryonic development events, which may result in intergenerational, even transgenerational, epigenetic re-programming with transmission of adverse traits and characteristics to offspring. Here, we focus on the research progress relating to diseases that originated from events in the gametes and early Embryos and the potential epigenetic mechanisms involved.

关键词： gamete Embryo acquired inheritance intergenerational/transgenerational transmission epigenetic modification

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SERK Receptor-like Kinases Control Division Patterns of Vascular Precursors and Ground Tissue Stem Cells during Embryo Development in Arabidopsis

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Molecular Plant 2019年第7期12卷 984-1002页

作者： Huiqiang Li Zeping Cai Xiaojuan Wang Meizhen Li Yanwei Cui Nan Cui Fei Yang Mingsong Zhu Junxiang Zhao Wenbin Du Kai He Jing Yi Frans E.Tax Suiwen Hou Jia Li Xiaoping Gou Ministry of Education Key Laboratory of Cell Activities and Stress Adaptations School of Life Sciences Lanzhou University Lanzhou Gansu 730000 China College of Forestry Hainan University Danzhou Hainan 571737 China Department of Molecular and Cellular Biology University of Arizona Tucson AZ 85721 USA

During Embryo development, the vascular precursors and ground tissue stem cells divide to renew them-selves and produce the vascular tissue, endodermal cells, and cortical cells. However, the molecular mech-anisms regulating division of these stem cells have remained largely elusive. In this study, we show that loss of function of SOMATIC EmbryoGENESIS RECEPTOR-UKE KINASE (SERK) genes results in aberrant em-bryo development. Fewer cortical, endodermal, and vascular cells are generated in the Embryos of serk1 serk2bak1 triple mutants. WUSCHEL-RELATED HOMBOBOX5 (WOXS) is ectopically expressed in vascular cells of serkl serk2 bak1 Embryos. The first transverse division of vascular precursors in mid-globular em-bryos and second asymmetric division of ground tissue stem cells in early-heart Embryos are abnormally altered to a longitudinal division. The Embryo defects can be partially rescued by constitutively activated mitogen-activated protein kinase (MAPK) kinase kinase YODA (YDA) and MAPK kinase MKK5. Taken together, our results reveal that SERK-mediated signals regulate division patterns of vascular precursors and ground tissue stem cells, likely via the YDA-MKK4/5 cascade, during Embryo development.

关键词： Embryo ground tissue stem cell MKK4 MKK5 SERKs vascular precursors

Basal Serum Anti-Miillerian Hormone Level as a Predictor of Clinical Outcomes in Freezing-all Embryo Transfer Program

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Current Medical Science 2018年第5期38卷 861-867页

作者： Xiao-lan LI Rui HUANG Cong FANG Xiao-yan LIANG Reproductive Medicine Research Center Sixth Affiliated HospitalSun Yat-sen UniversityGuangzhou 510655China

As a novel biomarker,there is inconsistent evidence regarding the association between anti-Miillerian hormone (AMH) and live birth rate in freezing-all Embryo transfer cycles.We aim to assess the prognostic effect of baseline AMH on clinical outcomes,especially live birth rate in freezing-all Embryo transfer cycles.A total of 828 non-polycystic ovary patients that underwent their first frozen-thawed Embryo transfers in our center between January 2010 and January 2015 were recruited in this retrospective analysis.Patients were stratified into three groups based on their baseline AMH concentration:low AMH group (5.8 ng/mL).The results showed that low AMH level was associated With adverse clinical outcomes.The differences in implantation rate (21.9% vs.43.2% vs.58.8%,PEmbryos transferred,etiologies of infertility),differences in live birth rate,clinical pregnancy rate and implantation rate between groups remained significant.The further age subgroup analysis demonstrated that low AMH concentration was significantly associated with poor outcomes both in young and advanced patients.The area under the curve for serum AMH,age,AFC and FSH were 0.635,0.634,0.615 and 0.543 respectively,for predicting live birth.In conclusion, baseline AMH was an independent prognostic factor of live birth rate of freezing-all Embryo transfers,but its predictive value on live birth rate was of limited clinical value.

关键词： anti-Miillerian hormone freezing-all Embryo transfer live birth rate