检索结果-南通市图书馆

Neuroscience Bulletin 2019年第2期35卷 205-215页

作者： Lei Guo Jochem StormmesAND Zheng Fang Qingbin Zhu Rawien Balesar Joop van Heerikhuize Arja Sluiter Dick Swaab Ai-Min Bao Department of Neurobiology and Department of Neurology of the Second Affiliated Hospital Institute of NeuroscienceNHC and CAMS Key Laboratory of Medical NeurobiologyZhejiang University School of Medicine Netherlands Institute for Neuroscience Institute of the Royal Netherlands Academy of Arts and Sciences Department of Neurology Sir Run Run Shaw HospitalZhejiang University School of Medicine

The locus coeruleus(LC) has been studied in major depressive disorder(MDD) and bipolar disorder(BD). A major problem of immunocytochemical studies in the human LC is interference with the staining of the immunocytochemical end-product by the omnipresent natural brown pigment neuromelanin. Here, we used a multispectral method to untangle the two colors: blue immunocytochemical staining and brown neuromelanin.We found significantly increased tyrosine hydroxylase(TH) in the LC of MDD patients—thus validating the method—but not in BD patients, and we did not find significant changes in the receptor tyrosine-protein kinase ErbB4 in the LC in MDD or BD patients. We observed clear co-localization of ErbB4, TH, and neuromelanin in the LC neurons. The different stress-related molecular changes in the LC may contribute to the different clinical symptoms in MDD and BD.

关键词： Major depressive disorder Bipolar disorder Tyrosine hydroxylase ErbB4 locus coeruleus

Analysis of promoter activity reveals that GmFTL2 expression differs from that of the known Flowering locus T genes in soybean

在线全文

学校读者我要写书评

暂无评论

The Crop Journal 2017年第5期5卷 438-447页

作者： Limin Liu Xiaomei Zhang Fulu Chen Asia Adam Elzamzami Mahi Xiaoxia Wu Qingshan Chen Yong-Fu Fu College of Agriculture Northeast Agricultural University MOA Key Lab of Soybean Biology (Beijing) National Key Facility for Crop Gene Resources and Genetic Improvement Institute of Crop ScienceChinese Academy of Agricultural Sciences

Regulation of flowering is one of the key issues in crop yield. The Floiuering Loews T(FT) gene is a well-known florigen, which integrates various signals from multiple flowering-regulation pathways to initiate flowering. We previously reported that there are at least six FT genes(GmFTLl-6) in soybean displaying flowering activity. However, the individual functions of genes GmFTLl-6 remain to be identified. In this study, we cloned the GmFTL2 promoter(GmFTLpro) from soybean(Glycine max) cultivar Tianlong 1 and analyzed its motifs bioinformatically and its expression patterns using both a transgenic approach and quantitative RT-PCR(qRT-PCR). In GmFTLpro::GUS transgenic lines, GUS signals were enriched in cotyledons, hypocotyledons, pollen, embryos, and root tips in a photoperiod-independent manner. qRT-PCR confirmed the GUS reporter results. Our results suggest that GmFTL2 expression is regulated by developmental and tissue-specific clues and plays roles in seedling establishment and the development of micro game tophytes, embryos, and roots.

关键词： Flowering locus T Cotyledon Microgametophyte Embryo Root tip

Fine-Mapping of qTGW1.2a, a Quantitative Trait locus for 1000-Grain Weight in Rice

在线全文

学校读者我要写书评

暂无评论

Rice science 2019年第4期26卷 220-228,I0002-I0004,I0010,I0015页

作者： WANG Wenhui WANG Linlin ZHU Yujun FAN Yeyang ZHUANG Jieyun State Key Laboratory of Rice Biology/Chinese National Center for Rice Improvement China National Rice Research Institute

Thousand-grain weight (TGW) is a key component of grain yield in rice. This study was conducted to validate and fine-map qTGW1.2a, a quantitative trait locus for grain weight and grain size previously located in a 933.6-kb region on the long arm of rice chromosome 1. Firstly, three residual heterozygotes (RHs) were selected from a BC2F11 population of the indica rice cross Zhenshan 97 (ZS97)///ZS97//ZS97/Milyang 46. The heterozygous segments in these RHs were arranged successively in physical positions, forming one set of sequential residual heterozygotes (SeqRHs). In each of the populations derived, non-recombinant homozygotes were identified to produce near isogenic lines (NILs) comprising the two homozygous genotypes. The NILs were tested for grain weight, grain length and grain width. QTL analyses for the three traits were performed. Then, the updated QTL location was followed for a new run of SeqRHs identification-NIL development-QTL mapping. Altogether, 11 NIL populations derived from four sets of SeqRHs were developed and used. qTGW1.2a was finally delimitated into a 77.5-kb region containing 13 annotated genes. In the six populations segregating this QTL, which were in four generations and were tested across four years, the allelic direction of qTGW1.2a remained consistent and the genetic effects were stable. For TGW, the additive effects ranged from 0.23 to 0.38 g and the proportions of phenotypic variance explained ranged from 26.15% to 41.65%. These results provide a good foundation for the cloning and functional analysis of qTGW1.2a.

关键词： fine mapping grain weight minor effect quantitative trait locus rice grain length grain width

Modulation of neuronal activity of cerebellar fastigial nucleus by locus coeruleus stimulation in the rat

在线全文

学校读者我要写书评

暂无评论

Chinese Science Bulletin 1998年第11期43卷 940-944页

作者： Wang Tao Wang Jianjun Cheng Hong Li Hongzhao Yu Qixiang Nanjing Univ Dept Biol Sci & Technol Nanjing 210093 Peoples R China

The effects of stimulating locus coeruleus (LC) on neuronal activity of cerebellar fastigial nucleus (FN) was investigated. Stimulation of LC elicited inhibitory, excitatory and biphasic (inhibition-excitation) responses from FN cells. The majority of responsive cells showed an inhibitory response with a latency of less than 10 ms. Injection of α adrenoreceptor antagonists phentolamine (ⅳ) could block the inhibitory response of FN cells to the LC stimulation, but propranolol (ⅳ), a β adrenoreceptor antagonist, could not. These results suggest that LC-cerebellar noradrenergic afferent fibers may be involved in the cerebellar sensorimotor integration process by exerting their modulatory action on the cerebellar nuclear cells’ activities.

关键词： locus coeruleus cerebellar fastigial nucleus coeruleo-cerebellar pathway noradrenergic fibers.

CHANGES IN FIRING RATE OF NORADRENERGIC NEURONS IN locus COERULEUS FOLLOWING STIMULATION OF MEDIAL PREOPTIC AREA IN FEMALE RATS AT DIFFERENT STAGES OF THE ESTROUS CYCLE

在线全文

学校读者我要写书评

暂无评论

Chinese Science Bulletin 1991年第4期36卷 333-338页

作者：朱笛霓孙启新赵建础 Shaanxi Academy of Traditional Chinese Medicine and Pharmacy Xi'an 710003 PRC

Several studies showed that sex hormone receptors are in close relationship with classical neurotransmitter neurons especially catecholaminergic(CA) neurons. There are many gonadotrophin releasing hormone (GnRH) neuro... 详细信息

关键词： medial preoptic area noradrenergic neuron locus coeruleus estrus diestrus rat.

COMPARISON OF THE EFFECTS OF MEDIAL PRE OPTIC AREA AND VENTROMEDIAL NUCLEUS OF HYPOTHALAMUS ON THE DISCHARGES OF NORADRENERGIC NEURONS IN locus COERULEUS IN FEMALE RATS AT DIFFERENT STAGES OF ESTROUS CYCLE

在线全文

学校读者我要写书评

暂无评论

Chinese Science Bulletin 1992年第22期37卷 1915-1919页

作者：朱笛霓赵建础 Shaanxi Academy of Traditional Chinese Medicine and Pharmacy Xi'an 710003 PRC

In the structure of brain the medial preoptic area (MPO) and ventromedial nucleus of hypothalamus (VMN)are the two parts where the estrogen receptors concentrate but have opposite effects on sexual behavior. Recently estrogen receptors have been known to be located in the locus coeruleus(LC), where noradrenergic(NE) fibres terminate in the hypothalamus arising from cell bodies. The discharge rate of NE-ergic neurons in LC can be increased by electrical stimulation of MPO in a female rat at estrus stage, and decreased at diestrus stage. These studies help us to infer that VMN might have some modulation influence on NE-ergic neurons in LC. In order to study the pattern activities

关键词： medial preoptic area ventromedial nucleus of hypothalamus locus coeruleus estrus diestrus rat

PRINCIPAL SHEARING STRESS locus THEORY OF AXISYMMETRICALLY PLASTIC FLOW

在线全文

学校读者我要写书评

暂无评论

Chinese Science Bulletin 1992年第22期37卷 1924-1928页

作者：王仲仁胡卫龙 Department of Material Science and Technology Harbin Institute of Technology Harbin 150006 PRC

The slip-line method is one of the fundamental solving methods for the plastic deformation. However, the existing slip-fine theory, strictly speaking, is only confined to solving a rigid-perfectly plastic material under the plane strain conditions. Although some authors (such as Shield) have made an extension to the axisymmetdeally plastic problems with the aid of the "perfect plasticity" hypothesis, more axisymmetric problems still cannot be solved by the method. For this reason, in the present note, a new hypothesis is proposed for analysing the axisymmetric plastic problems, and then, according to this hypothesis,

关键词： axisymmetrically plastic flow principal shearing stress locus

DETERMINATION OF PHASE EQUILIBRIA AND CRITICAL locus OF CO_2-HCFC22 SYSTEM

在线全文

学校读者我要写书评

暂无评论

Chinese Journal of Chemical Engineering 1993年第1期1卷 54-58页

作者：卞白桂王延儒时钧

1 INTRODUCTIONHydrochlorofluorocarbons(HCFCs)and hydrofluorocarbons(HFCs)are alternatives tochlorofluorocarbons(CFCs),such as CFC12(freon 12),which has been widely used asan aerosol agent and working fluid of domestic1:ICthdflC(CHCIFZ,HCFC22,R22)is one of the potential alternatives.It Is also one of the po-tentlal entralner for supercrltlcal fluid CO,extraction.

关键词： phase equilibria critical locus hydrochlorofluorocarbons carbon dioxide

Functional analysis of alternative splicing of the FLOWERING locus T orthologous gene in Chrysanthemum morifolium

在线全文

学校读者我要写书评

暂无评论

Horticulture Research 2016年第1期3卷 51-58页

作者： Yachao Mao Jing Sun Peipei Cao Rong Zhang Qike Fu Sumei Chen Fadi Chen Jiafu Jiang College of Horticulture Nanjing Agricultural UniversityNanjing210095China

As the junction of floral development pathways,the FLOWERING locus T(FT)protein called‘florigen’plays an important role in the process of plant flowering through signal integration.We isolated four transcripts encoding different isoforms of a FT orthologous gene CmFTL1,from Chrysanthemum morifolium cultivar‘Jimba’.Sequence alignments suggested that the four transcripts are related to the intron 1.Expression analysis showed that four alternative splicing(AS)forms of CmFTL1 varied depending on the developmental stage of the flower.The functional complement experiment using an Arabidopsis mutant ft-10 revealed that the archetypal and AS forms of CmFTL1 had the function of complementing late flower phenotype in different levels.In addition,transgenic confirmation at transcript level showed CmFTL1 and CmFTL1ast coexist in the same tissue type at the same developmental stage,indicating a post-transcriptional modification of CmFTL1 in Arabidopsis.Moreover,ectopic expression of different AS forms in chrysanthemum resulted in the development of multiple altered phenotypes,varying degrees of early flowering.We found that an alternative splicing form(CmFTL1-astE134)without the exon 2 lacked the ability causing the earlier flower phenotype.The evidence in this study indicates that complex alternative processing of CmFTL1 transcripts in C.morifolium may be associated with flowering regulation and hold some potential for biotechnical engineering to create early-flowering phenotypes in ornamental cultivars.

关键词： locus analysis cultivar

Rheumatology and functional genome analysis in East Asia

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Rheumatology & Autoimmunity 2022年第1期2卷 1-4页

作者： Keishi Fujio Department of Allergy and Rheumatology Graduate School of MedicineThe University of TokyoTokyoJapan

Rheumatic diseases differ in severity and outcome across ethnic groups.Although genetic predisposition is an important factor influencing the development of rheumatic diseases,genomic data to date have been accumulated mainly in Europeans.However,East Asia has become an influential center for genetic studies worldwide,and there is also a growing need for a functional genome database for East Asians.Expression quantitative trait locus data for immune cell subsets can be extremely useful in interpreting the function of disease-associated genetic polymorphisms.The development of a functional genome database for East Asians is expected to make a significant contribution to the understanding and stratification of the pathogenesis of rheumatic diseases in this population in the future.

关键词： ethnicity expression quantitative trait locus functional genome analysis rheumatic diseases