Development of Preparative Chromatography for Proteomic Approach of Mycorrhizal Symbiosis

作     者：Tomoko Matsubara Takaaki Ishii 

作者机构：Graduate School of Life and Environmental Sciences Kyoto Prefectural University Kyoto Japan 

出 版 物：《Advances in Biological Chemistry》 (生物化学进展（英文）)

年 卷 期：2015年第5卷第1期

页      面：16-23页

学科分类：1002[医学-临床医学] 100214[医学-肿瘤学] 10[医学] 

主　　题：24 kDa Protein Mycorrhizal Fungi Preparative Chromatography Proteomic Analysis Symbiosis 

摘      要：Although mechanism of symbiosis between arbuscular mycorrhizal fungi (AMF) and host plants has been investigated by genetic analysis, very little knowledge has been obtained because genome analysis of AMF is not perfect yet. Thus, we tried to develop mass purification of proteins using preparative chromatography in order to accelerate roteomic analysis of proteins related to mycorrhizal symbiosis, such as 24 and 53 kDa proteins. In particular, our data showed that 53 kDa proteins would be restrictively expressed when mycorrhizal fungi and host plants were stressed. However, 24 kDa proteins, which appear to be a usable indicator for the existence of various my-corrhizal fungi, were habitually detected in not only AMF but also other mycorrhizal fungi such as ectomycorrhizal fungi (EF). Moreover, we discovered new preparative chromatographical techniques for isolation and mass purification of those proteins. We are convinced that this chromato-graphical technique will greatly contribute to proteomic approach of mycorrhizal symbiosis.