Vasoprotective effects of rice bran water extract on rats fed with high-fat diet

作     者：Narongsuk Munkong Pintusorn Hansakul Bhornprom Yoysungnoen Ariyaphong Wongnoppavich Seewaboon Sireeratawong Noppamat Kaendee Nusiri Lerdvuthisopon 

作者机构：Department of MedicineSchool of MedicineUniversity of Phayao Department of Preclinical ScienceFaculty of MedicineThammasat University Department of BiochemistryFaculty of MedicineChiang Mai University Department of PharmacologyFaculty of MedicineChiang Mai University Department of Health PromotionFaculty of Health ScienceSrinakharinwirot University 

出 版 物：《Asian Pacific Journal of Tropical Biomedicine》 (亚太热带生物医学杂志（英文版）)

年 卷 期：2016年第6卷第9期

页      面：778-784页

核心收录：

学科分类：0710[理学-生物学] 0831[工学-生物医学工程（可授工学、理学、医学学位）] 1006[医学-中西医结合] 10[医学] 100602[医学-中西医结合临床] 

基　　金：Supported by Research Unit Faculty of Medicine Thammasat University and the Higher Education Research Promotion and National Research University Project of Thailand(Grant No.2-17/2013 and 2014-76 respectively) 

主　　题：Rice bran Metabolic syndrome Cardiovascular disease Endothelial nitric oxide synthase Nuclear factor-kappa B Cluster of differentiation 36 

摘      要：Objective:To elucidate the protective effects of rice bran water extract on the expression of endothelial nitric oxide synthase(e NOS),nuclear factor-kappa B(NF-k B),and a cluster of differentiation 36(CD36) in the vasculature of high-fat diet-fed rats.Methods:Male Sprague-Dawley rats were divided into three groups.Group I served as control,Group II was treated with high-fat diet,and Group III was treated with high-fat diet and rice bran water extract at 2 205 mg/kg/day.After four weeks,the metabolic parameters,malondialdehyde as a marker of oxidative stress,and histological features of the aorta were evaluated.The levels of transcripts and proteins in aorta were determined by real-time PCR and Western blot analysis,respectively.Results:In comparison with the Group II,rice bran water extract administration resulted in a significant reduction in body weight,visceral fat tissue weights,blood glucose levels,and serum total-cholesterol and free fatty acid levels in Group III.Serum triglyceride levels tended to decrease in the Group III.Also,rice bran water extract administration obviously decreased malondialdehyde levels in both serum and aorta.Interestingly,rice bran water extract treatment demonstrated a significant up-regulation of e NOS expression and down-regulation of NF-k B p65 and CD36 expressions.Nonetheless,all groups showed normal histology of aorta.Conclusions:Rice bran water extract exhibited vasoprotective effects in the high-fat diet-induced obesity condition by modulating the expression of e NOS,NF-kB,and CD36 and metabolic parameters.