STUDIES ON THE RNA POLYMERASE ACTIVITY ASSOCIATED WITH RICE RAGGED STUNT VIRUS

作     者：陆惠华 龚祖埙 曹天钦 LU HUIHUA;GONG ZUXUN;CAO TIANQIN Shanghai Institute of Biochemistry, Academia Sinica

作者机构：Shanghai Institute of Biochemistry Academia Sinica Academia Sinica 

出 版 物：《中国科学B辑(英文版)》 (Science in China,Ser.B)

年 卷 期：1988年第31卷第5期

页      面：63-66页

核心收录：

学科分类：1002[医学-临床医学] 100214[医学-肿瘤学] 10[医学] 

基　　金：Project supported by the National Natural Science Foundation of China. 

主　　题：declined accumulated incubation activate insoluble RNA Transcription prior intact implied 

摘      要： In an in vitro transcription system, the intact virion of rice ragged stunt virus (RRSV) coulddirect the synthesis of an acid-insoluble material. This indicated that the virus might contain RNA poly-merase activity, with an optimum range of activity at 30-40℃, maximal at 35℃. Divalent cationswere essential for the activity. Transcription products accumulated in the incubation period from 0 to4 h In addition, the polymerase activity declined if the virus was heat-shocked or treated with chy-motrypsin, which implied that no prior treatment was required to activate transcription catalyzed byRRSV.