Construction of cytopathic PK-15 cell model of classical swine fever virus

作     者：WU Haixiang, ZHANG Chuyu, ZHENG Congyi, WANG Jiafu, PAN Zishu, LI Lei, CAO Shen & Yl GuanghuiInstitute of Virology, College of Life Sciences, Wuhan University, Wuhan 430072, ChinaCorrespondence should be addressed to Zhang Chuyu (e-mail: avlab@ ***) 

作者机构：Institute of Virology College of Life Sciences Wuhan University Wuhan 430072 China 

出 版 物：《Chinese Science Bulletin》 (Chin. Sci. Bull.)

年 卷 期：2003年第48卷第9期

页      面：887-891页

核心收录：

学科分类：090601[农学-基础兽医学] 09[农学] 0906[农学-兽医学] 

基　　金：This work was supported by the National Natural Science Foundation of China(Grant Nos.30240080 and 30170214) the National Basic Research Developmental Projects(Grant No.G1999011900) 

主　　题：PK-15 细胞结构 病变细胞 传统猪狂热病毒 CSFV cDNA 基因克隆 

摘      要：No cytopathic effect (CPE) can be observed on classical swine fever virus (CSFV) infected cell culture in vitro. This brings an obstacle to the researches on reciprocity between CSFV and host cells. Based on the construction of full-length genomic infectious Cdna clone of Chinese CSFV standard virulent Shimen strain, partial deletion is intro- duced into genomic Cdna to obtain a 7.5 kb subgenomic Cdna. A new subgenomic CSFV is derived from transfection with the subgenomic Cdna on PK-15 cells pre-infected by CSFV Shimen virus. Typical CPE induced by this subgenomic virus is observed on PK-15 cells. Coexistence of wild- type and subgenomic virus in cytopathic cell culture is dem- onstrated by RT-PCR detection in cytopathic cells. For conclusion, the construction of cytopathic cell model exploited a new way for researches on the molecular mechanism of CSFV pathogenesis.