DEVELOPMENT OF A VERSATILE SENSING MEMBRANE FOR IMMUBILIZATION OF PROTEIN AND A NEW IMMOBILIZATION METHOD

作     者：Y.K. Zhou, J. W. Yuan,S. Q. Xu, B. H. Shu(Department of Environmental Medicine,Tongji Medical University,Wuhan 430030, China)P. R. H. Kooyman(Department of Applied Physics,MESA Institute, University of Twente, P. O. Box 217,7500 AE Enschede, The Netherlan 

出 版 物：《Chinese Journal of Biomedical Engineering(English Edition)》 (中国生物医学工程学报（英文版）)

年 卷 期：1996年第5卷第4期

页      面：155-162页

学科分类：08[工学] 080501[工学-材料物理与化学] 0805[工学-材料科学与工程（可授工学、理学学位）] 080502[工学-材料学] 

主　　题：immobilization or protein biotin-avidin system sensingmembrane biosensor optical immunosensor 

摘      要：This paper describes a versatile sensing membrane for immobilizationof proteins (antibodies, antigens, enzymes and so on) and a new immobolizationmethod The membrane was developed with polyacrylaminde gel and blotinstrep-tavidin system. Streptavidin was bond to the polyacryamide gel with 1-ethyl-3-(3-dimethylaminopropyl carbodimide (EDAC), which results in a streptavidin membrane, and a biotinalyted ligand was then easily linked to the gel via the streptavidin *** streptavidin membrane (the thickness or 500urn) was used toimmobilize anti-human chorionic gonadotrophin antibody (Q hCG). The number orQbCG in the membrane was about 1011 per mm2 of the membrane surface. The membrane was used as a sensing membrane or optical immunosensor (Mach-Zehnder interferomter). It had a good response at the concentration or 10 afl/L hCG.