Role of phenolics as antioxidants,biomolecule protectors and as anti-diabetic factors-Evaluation on bark and empty pods of Acacia auriculiformis

作     者：Arumugam Sathya Perumal Siddhuraju 

作者机构：Bioresource Technology LabDepartment of Environmental SciencesBharathiar University 

出 版 物：《Asian Pacific Journal of Tropical Medicine》 (亚太热带医药杂志（英文版）)

年 卷 期：2012年第5卷第10期

页      面：757-765页

核心收录：

学科分类：1008[医学-中药学(可授医学、理学学位)] 1006[医学-中西医结合] 100602[医学-中西医结合临床] 10[医学] 

基　　金：University Grants Commission (UGC) New Delhi India(F.No.34-259\2008)for the financial assistance 

主　　题：Phenolics Bark Empty pods Acacia auriculiformis Antioxidant activity TypeⅡdiabetes 

摘      要：Objective:To search for an efficient and inexpensive source of phytoconstituents with antioxidant potential and health promoting traits from bark and empty pods of Acacia auriculiformis （***）.Methods:Samples of bark and empty pod extracts were analyzed for bioactives（phenolics,flavonoids and proanthocyanidins） and subjected to free radical scavenging activity on DPPH*,ABTS?,OH?,O2*,and NO along with the determination of reducing power, iron chelating activity and peroxidation *** action of extracts on biomolecules and cell membranes were evaluated by DNA nicking assay and haemolysis inhibition assay respectively,α-amylase andα-glucosidase inhibitory potentials were also determined. Results:All the bioactives analyzed were higher in bark（B） than empty pods（EP）[TPC:B （574.51±16.11）;EP（96.80±3.45） mg GAE/***:B（94.71±7.65）;EP（24（?）87±20.45） mg RE/g. Proanthocyanidins:B（2.81±0.31）;EP（1.25±0.01） mg LE/100 g DM]except *** the extracts showed higher quenching capacity on DPPH and ABTS（DPPH:B（0.21±0.01）;EP（1.51±0.17） g extract/g ***:B（111 519.14±79 340.91）;EP（80 232.55±32 894.12） mmol TE/g） with the FRAP of B（84 515.63±3 350.69） and EP（47 940.79±1 257.60） mmol Fe（（?））/*** chelation was not *** addition,they showed lower quenching activity on OH?（B（48.95±1.72）;EP（34.94±1.62）%） and equivalent quenching on O2?（B（53.47±3.92）;EP（24.41±2.61）%）,NO（B（49.04±5.04）; EP（51.00±5.13）%）,peroxidation inhibition（B（67.50±5.50）;EP（55.l±2.3）%） and antihaemolytic potential（B（87.60±6.84）%） towards authentic antioxidant ***,Empty pod extracts are devoid of antihaemolytic *** the extracts showed dose dependent DNA *** this,bark and empty pod extracts exhibited dual inhibiting potential againstα-amylase andα-glucosidase ***:On summarization,it insinuated that both bark and empty pods can be us