Larvicidal,ovicidal and pupicidal activities of Gliricidia sepium(Jacq.)(Leguminosae) against the malarial vector,Anopheles stephensi Liston(Culicidae:Diptera)

作     者：Kaliyamoorthy Krishnappa Shanmugam Dhanasekaran Kuppusamy Elumalai 

作者机构：Unit of EntomotoxicityDepartment of Zoology Govt.Thirumagal Mills College Vellore-632607 TamilnaduIndia Center for Entomotoxicity StudiesDepartment of ZoologyAnnamalai UniversityAnnamalainagar-608 002 TamilnaduIndia 

出 版 物：《Asian Pacific Journal of Tropical Medicine》 (亚太热带医药杂志（英文版）)

年 卷 期：2012年第5卷第8期

页      面：598-604页

核心收录：

学科分类：1008[医学-中药学(可授医学、理学学位)] 10[医学] 

基　　金：Higher Authorities for the grant of financial assistance with the Award of University Research Fellowship(Ref.No.No.H5/2009-2010/URF Order of the Vice chancellore,23011/2009) Professor and Head,Department of Zoology,Annamalai University for the laboratory facilities provided 

主　　题：Anopheles stephensi Gliricidia sepium Crude extract Fractions Larvicidal activity Ovicidal activity Pupicidal activity 

摘      要：Objective:To investigate the potentiality of mosquitocidal activity of Gliricidia sepium（***） （Jacq.）（Leguminosae）.Methods:Twenty five early third instar larvae of Anopheles stephensi （***） were exposed to various concentrations（50-250 ppm） and the 24 h LC50 values of the *** extract was determined by probit *** ovicidal activity was determined against *** to various concentrations ranging from 25-100 ppm under laboratory *** eggs hatchabilily was assessed 48 h post *** pupicidal activity was determined against *** to various concentrations ranging from 25-100 *** of each pupa was recorded after 24 h of exposure to the ***:Results pertaining to the experiment clearly revealed that ethanol extract showed significant larvicidal,ovicidal and pupicidal activity against the *** activity of ethanol extracts of *** showed maximum mortality in 250 ppm concentration（96.0±2.4）%.Furthermore,the LC50 was found to be 121.79 and the LC50 value was recorded to be 231.98 *** activity of ethanol extract was assessed by assessing the egg *** concentration of both solvent extracts exhibited 100%ovicidal ***,pupae exposed to different concentratioiLs of ethanol extract were found dead with 58.10%adult emergence when it was treated with 25 ppm ***,18.36（n=30;61.20%）;21.28（70.93） and 27.33（91.10） pupal mortality was recorded from the experimental pupae treated with 50,75 and 100 ppm concentration of extracts. Three fractions have been tested for their larvicidal activity of which the Fraction 3 showed the LC50 and LC90 values of 23.23 and 40.39 *** regard to the ovicidal effect fraction 3 showed highest ovicidal activities than the other two ***,there were no hatchabilily was recorded above 50 ppm（100%egg mortality） in the experimental gro