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Larvicidal,ovicidal and pupicidal activities of gliricidia sepium(Jacq.)(Leguminosae) against the malarial vector,Anopheles stephensi Liston(Culicidae:Diptera)

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Asian Pacific Journal of Tropical Medicine 2012年第8期5卷 598-604页

作者： Kaliyamoorthy Krishnappa Shanmugam Dhanasekaran Kuppusamy Elumalai Unit of Entomotoxicity Department of Zoology Govt.Thirumagal Mills College Vellore-632607 TamilnaduIndia Center for Entomotoxicity Studies Department of ZoologyAnnamalai UniversityAnnamalainagar-608 002 TamilnaduIndia

Objective:To investigate the potentiality of mosquitocidal activity of gliricidia sepium（***）（Jacq.）（Leguminosae）.Methods:Twenty five early third instar larvae of Anopheles stephensi （***） were exposed to various concentrations（50-250 ppm） and the 24 h LC50 values of the *** extract was determined by probit *** ovicidal activity was determined against *** to various concentrations ranging from 25-100 ppm under laboratory *** eggs hatchabilily was assessed 48 h post *** pupicidal activity was determined against *** to various concentrations ranging from 25-100 *** of each pupa was recorded after 24 h of exposure to the ***:Results pertaining to the experiment clearly revealed that ethanol extract showed significant larvicidal,ovicidal and pupicidal activity against the *** activity of ethanol extracts of *** showed maximum mortality in 250 ppm concentration（96.0±2.4）%.Furthermore,the LC50 was found to be 121.79 and the LC50 value was recorded to be 231.98 *** activity of ethanol extract was assessed by assessing the egg *** concentration of both solvent extracts exhibited 100%ovicidal ***,pupae exposed to different concentratioiLs of ethanol extract were found dead with 58.10%adult emergence when it was treated with 25 ppm ***,18.36（n=30;61.20%）;21.28（70.93） and 27.33（91.10） pupal mortality was recorded from the experimental pupae treated with 50,75 and 100 ppm concentration of extracts. Three fractions have been tested for their larvicidal activity of which the Fraction 3 showed the LC50 and LC90 values of 23.23 and 40.39 *** regard to the ovicidal effect fraction 3 showed highest ovicidal activities than the other two ***,there were no hatchabilily was recorded above 50 ppm（100%egg mortality） in the experimental gro

关键词： Anopheles stephensi gliricidia sepium Crude extract Fractions Larvicidal activity Ovicidal activity Pupicidal activity

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In vitro activities of acetonic extracts from leaves of three forage legumes(Calliandra calotyrsus,gliricidia sepium and Leucaena diversifolia) on Haemonchus contortus

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Asian Pacific Journal of Tropical Medicine 2011年第2期4卷 125-128页

作者： Wabo Pone J Kenne Tameli Florence Mpoame Mbida Pamo Tedonkeng E Bilong Bilong CF Department of Animal Biology Faculty of ScienceUniversity of DschangP O Box 067 DschangCameroon Department of Animal Science Faculty of Agronomy and Agricultural SciencesUniversity of DschangP O Box 222 DschangCameroon Department of Animal Biology and Physiology Laboratory of General BiologyFaculty of ScienceUniversity of Yaounde 1PO Box 812 YaoundeCameroon

Objective:To assess ovicidal activity of three acetonic extracts from the leaves of three forage legume,Calliandra calotyrsus(***),gliricidia sepium(***) and Leucaena diversifolia(***) in vitro on Haemonchus contortus(***).Methods:Eggs were exposed for 24 hours to five different concentrations(0.075,0.15,0.3,0.6 and 1.25 mg/mL) of acetonic extracts at room temperature(24℃).Distilled water and 0.4%Tween were used in the bioassay as negative ***:The later did not affect embryonation and egg hatching of ***,significant effects were obtained with the acetonic extracts of leaves of all three plants and the maximum activity was observed with the highest concentration (1.25 mg/mL).The acetonic extract of *** was found to be more active(2.9%and 0.0% for embryonation and egg hatching,respectively) than the other substances 16.5%and 33.5%, respectively for ***,33.7%and 33.3%,respectively for L ***: These results suggest that the three forage legumes do possess ovicidal properties and further studies on larvae should be carried out.

关键词： Extract Calliandra calotyrsus gliricidia sepium Leucaena diversifolia Mebendazole Haemonchus contortus Cameroon

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Productivity of Soil Fertilised with Fermented Calliandra, gliricidia and Leucaena Browses and Maize Forage

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Journal of Agricultural Science and Technology(B) 2013年第3期3卷 199-203页

作者： Habib Kato Felix Budara Bareeba Elly Nyambobo Sabiiti Department of Agriculture Kyambogo University P.O. Box 1 Kyambogo Uganda Department of Animal Science Makerere University P.O. Box 7062 Kampala Uganda Department of Crop Science Makerere University P.O. Box 7062 Kampala Uganda

Fermented Callliandra, gliricidia and Leucaena browses and maize material （milk stage） were applied to the soil to determine their effect on soil productivity. Hopi Red Dye Amaranthus （Amaranthus cruentus） was used as the test crop. Its DM yield was determined. The browse materials had higher total N and narrower C：N ratio than the maize material. Calliandra material had higher levels of insoluble fiber （ADF）, fiber bound N （ADFN） and lignin. Application of the browse and maize materials raised C, N, and C：N ratio of the soil in which they were applied compared to the control soil. The treated soils maintained higher levels of C and N and a narrower C：N ratio up to the third crop. Amaranthus DM yield was highest （P 〈 0.05） with browse treatments. Treatment with maize material did not have DM yield advantage over the control soil. Treatments with browse materials could have given higher yields because the materials had more N and fermentation could not only have increased the proportion of soluble N, but also degradability of the materials, thus making the N and other nutrients in the fermented materials available for plant growth. DM yield was highest （P 〈_ 0.05） with the first and third crops but lowest （P 〈 0.05） with the second crop. Plant growth in the second crop could have coincided with high demand for the N by the soil micro-organisms decomposing the added materials. During the third crop, decomposition of the readily degradable components of the added materials could have been complete and the N became available for plant growth. DM yield dropped （P 〈 0.05） with the fourth crop as the available N could have been depleted from the soil for plant growth.

关键词： Fermented Calliandra gliricidia Leucaena Amaranthus soil productivity.

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