Effects of Mercuric Chloride on Antioxidant System and DNA Integrity of the Crab Charybdis japonica

作     者：ZHANG Hongxia PAN Luqing MIAO Jingjing XU Chaoqun 

作者机构：The Key Laboratory of Mariculture Ministry of Education Ocean University of China Qingdao 266003 P. R. China 

出 版 物：《Journal of Ocean University of China》 (中国海洋大学学报（英文版）)

年 卷 期：2009年第8卷第4期

页      面：416-424页

学科分类：0710[理学-生物学] 090801[农学-水产养殖] 071010[理学-生物化学与分子生物学] 081704[工学-应用化学] 0908[农学-水产] 07[理学] 0707[理学-海洋科学] 08[工学] 0817[工学-化学工程与技术] 09[农学] 0815[工学-水利工程] 0824[工学-船舶与海洋工程] 

基　　金：supported by the 111 Project (B0804) the Agricultural Science and Technology Achievement Transfer Project of TianJin City of China (07050201) 

主　　题：DNA链断裂 过氧化氢酶 氯化汞 整性 螃蟹 日本蟳 谷胱甘肽过氧化物酶 海洋无脊椎动物 

摘      要：Mercury (Hg) is one of the commonly encountered heavy metals, which is widespread in inshore sediments of China. In order to investigate the toxicity of Hg on marine invertebrates, we studied the effects of the divalent mercuricion (Hg2+) (at two final concentrations of 0.0025 and 0.0050 mg L-1, prepared with HgCl2) on metallothionein (MT) content, DNA integrity (DNA strand breaks) and catalase (CAT) in the gills and hepatopancreas, antioxidant enzyme activities of superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPx), in the hemolymph, gills and hepatopancreas of the portunid crab Charybdis japonica for an experiment period up to 15 d. The results indicated that MT was significantly induced after 3 d, with a positive correlation with Hg2+ dose and time in the hepatopancreas and a negative correlation with Hg2+ dose and time in the gills. While CAT in the hemolymph was not detected, it increased in the hepatopancreas during the entire experiment; SOD and GPx in the three tissues were stimulated after 12 h, both attained peak value and then reduced during the experimental period. Meanwhile, DNA strand breaks were all induced significantly after 12 h. These results suggested the detoxification strategies against Hg2+ in three tissues of C. japonica.