Effect of hydroalcoholic extract of Hibiscus rosa sinensis *** in experimental colitis in rats

作     者：Amit D Kandhare Kiran S Raygude Pinaki Ghosh Arvindkumar E Ghule Tejas P Gosavi Sachin L Badole Subhash L Bodhankar 

作者机构：Department of PharmacologyPoona College of PharmacyBharati Vidyapeeth Deemed University Department of Homeopathic PharmacyHomeopathic Medical CollegeBharati Vidyapeeth Deemed University 

出 版 物：《Asian Pacific Journal of Tropical Biomedicine》 (亚太热带生物医学杂志（英文版）)

年 卷 期：2012年第2卷第5期

页      面：337-344页

学科分类：0710[理学-生物学] 0831[工学-生物医学工程（可授工学、理学、医学学位）] 1008[医学-中药学(可授医学、理学学位)] 1006[医学-中西医结合] 100602[医学-中西医结合临床] 10[医学] 

基　　金：Supported by the All India Council of Technical and Education(AICTE) India(Grant No.PG/GATE-SCM/2004-2005/G-39 Dated:11/02/2011) 

主　　题：Acetic acid Hibiscus rosa sinensis Inflammatory bowel disease Inflammatory cytokine Nitrite/nitrate Oxidative stress Ulcerative colitis Prednisolone 

摘      要：Objective:To elucidate the ameliorative effect of liydrualcuholic extract of leaves of Hibiscus rosa sinensis(HRS) in acetic acid induced experimental colitis in male wistar ***: The animals were administered with 2 mL acetic acid(4%) via intra *** animals were divided into various treatment groups(n=6).Prednisolone was used as standard drug and HRS was administered at a dose of 50,100 and 200 mg/kg *** control group of animals received 1 mL of vehicle(distilled water).Ulcer area,ulcer index,spleen weight,colon weight to length ratio, macroscopic score,haemalological parameters,colonic superoxide dismutase(SOD),glutathione(GSH),myeloperoxidase(MPO),malondialdchyde(MDA),tumor necrosis factor-α(TNK- α), nitric oxide(NO) and histological changes were recorded after the treatment regimen of 11 days. Results:Intrarectal instillation of acetic acid caused enhanced ulcer area,ulcer index,spleen weight,colon weight to length ratio,colonic MPO,MDA,NO and TNF-α It caused significant decreased level of SOD and *** with HRS for 7 days exhibited significant effect in lowering of oxidative stress,colonic NO,TNF- α and elevation of SOD and GSH at a dose of 100 and 200 mg/kg in acetic acid induced ***:The present investigation demonstrates HRS is of potent therapeutic value in the amelioration of expcrimenlal colilis in laboralory animals by inhibiting the proinflammatory mediator like NO and TNK-α.