Programmed co-assembly of DNA-peptide hybrid microdroplets by phase separation

作     者：Shengtao Yao Yue Liao Rizhao Pan Weiping Zhu Yufang Xu Yangyang Yang Xuhong Qian Shengtao Yao;Yue Liao;Rizhao Pan;Weiping Zhu;Yufang Xu;Yangyang Yang;Xuhong Qian

作者机构：School of PharmacyShanghai Key Laboratory of Chemical BiologyEast China University of Science and TechnologyShanghai 200237China State Key Laboratory of Bioreactor EngineeringEast China University of Science and Technology Shanghai 200237China 

出 版 物：《Chinese Chemical Letters》 (中国化学快报（英文版）)

年 卷 期：2022年第33卷第3期

页      面：1545-1549页

核心收录：

学科分类：0710[理学-生物学] 071010[理学-生物化学与分子生物学] 081704[工学-应用化学] 07[理学] 08[工学] 0817[工学-化学工程与技术] 0805[工学-材料科学与工程（可授工学、理学学位）] 080502[工学-材料学] 

基　　金：supported by the National Natural Science Foundation of China (No. 31600802) 

主　　题：Self-assembly Microdroplet DNA origami Phase separation Elastin-like polypeptides 

摘      要：Biopolymers, including DNA and peptides have been used as excellent self-assembling building blocks for programmable single-component or hybrid materials, due to their controlled molecular ***, combining two assembling principles of DNA-based programmability and peptide-based specific molecular interactions for hybrid structures to microscale has not yet been achieved. In this study,we describe a hybrid microsystem that emerges from the co-assembly of DNA origami structure and short elastin-like polypeptide conjugated oligonucleotides, and initiates liquid-liquid phase separation to generate microdroplets upon heating above the transition temperature. Moreover, the hybrid microdroplets are capable for guest molecule trapping and perform bi-/tri-enzymatic cascades with rate enhancements as open “microreactors. Our programmed assembled DNA-peptide microsystem represents a new combination of DNA nanotechnology and peptide science and opens potential application routes toward lifeinspired biomaterials.