Reduced grain chalkiness and its possible physiological mechanism in transgenic rice overexpressing L-GalLDH
Reduced grain chalkiness and its possible physiological mechanism in transgenic rice overexpressing L-GalLDH作者机构：College of Life Sciences Zhaoqing University Hunan Provincial Key Laboratory of Phytohormones and Growth Development Hunan Agricultural University College of Life Sciences South China Normal University
出 版 物：《The Crop Journal》 (作物学报（英文版）)
年 卷 期：2015年第3卷第2期
页 面：125-134页
核心收录：
基 金：supported by the National Natural Science Foundation of China (31270287, 31301244, 31471432) the Natural Science Foundation of Guangdong Province, China (2014A030313663, S2012010010680)
主 题：Grain chalkiness Ascorbic acid Abscisic acid Jasmonic acid Rice
摘 要：Chalkiness is one of the key factors determining rice quality and price. Ascorbic acid(Asc) is a major plant antioxidant that performs many functions in plants. L-Galactono-1,4-lactone dehydrogenase(L-Gal LDH, EC1.***.2.3) is an enzyme that catalyzes the final step of Asc biosynthesis in plants. Here we show that the L-Gal LDH-overexpressing transgenic rice, GO-2,which has constitutively higher leaf Asc content than wild-type(WT) plants, exhibits significantly reduced grain chalkiness. Higher foliar ascorbate/dehydroascorbate(Asc/DHA)ratios at 40, 60, 80, and 100 days of plant age were observed in GO-2. Further investigation showed that the enhanced level of Asc resulted in a significantly higher ribulose-1,5-bisphosphate(Ru BP) carboxylase/oxygenase(Rubisco) protein level in GO-2 at 80 days. In addition, levels of abscisic acid(ABA) and jasmonic acid(JA) were lower in GO-2 at 60, 80, and100 days. The results we present here indicate that the enhanced level of Asc is likely responsible for changing redox homeostasis in key developmental stages associated with grain filling and alters grain chalkiness in the L-Gal LDH-overexpressing transgenic by maintaining photosynthetic function and affecting phytohormones associated with grain filling.
维普期刊数据库
同方期刊数据库