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Analysis of the essential DNA region for OsEBP-89 promoter in response to methyl jasmonic acid

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Science China(Life Sciences) 2008年第3期51卷 280-285页

作者： LI Ang1,2, CHEN LiangLiang1,3, REN HaiYun3, WANG XueChen2, ZHANG HaiWen1,4 & HUANG Rong- Feng1,4 1 Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, China 2 National Laboratory of Plant Physiology and Biochemistry, College of Biological Sciences, China Agricultural University, Beijing 100094, China 3 College of Life Sciences, Beijing Normal University, Beijing 100875, China 4 National Key Facility of Crop Gene Resources and Genetic Improvement, Beijing 100081, China Biotechnology Research Institute Chinese Academy of Agricultural Sciences Beijing China National Laboratory of Plant Physiology and Biochemistry College of Biological Sciences China Agricultural University Beijing China College of Life Sciences Beijing Normal University Beijing China National Key Facility of Crop Gene Resources and Genetic Improvement Beijing China

In rice, the characterization of OsEBP-89 is inducible by various stress- or hormone-stimuli, including ethylene, abscisic acid (ABA), jasmonate acid (JA), drought and cold. Here, we report the investigation of essential DNA region within OsEBP-89 promoter for methyl jasmonic acid (MeJA) induction. PLACE analysis indicates that this promoter sequence contains multiple potential elements in response to various stimuli. First, we fused this promoter with GUS gene and analyzed its expression under MeJA treatment through Agrobacterium infiltration mediating transient expression in tobacco leaves. Our results revealed that this chimeric gene could be inducible by MeJA in tobacco leaves. To further de- termine the crucial sequences responsible for MeJA induction, we generated a series of deletion pro- moters which were fused with GUS reporter gene respectively. The results of transient expression of GUS gene driven by these mutant promoters show that the essential region for MeJA induction is po- sitioned in the region between -1200 and -800 in OsEBP-89 promoter containing a G-box (?1127), which is distinct from the essential region containing ERE (?562) for ACC induction. In all, our finding is helpful in understanding the molecular mechanism of OsEBP-89 expression under different stimuli.

关键词： OsEBP-89 essential DNA region methyl jasmonic acid transient assay promoter tobacco leaves

Systemic induction of H_(2)O_(2) in pea seedlings pretreated by wounding and exogenous jasmonic acid

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Science China(Life Sciences) 2005年第3期48卷 202-212页

作者： LIU Yan 1,2 , HUANG Weidong 1 , ZHAN Jicheng 1 & PAN Qiuhong 1 1. College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, China 2. College of Agronomy, Inner Mongolia Agricultural University, Huhhot 010019, China College of Food Science and Nutritional Engineering China Agricultural University Beijing100083 China College of Agronomy Inner Mongolia Agricultural University Huhhot010019 China College of Food Science and Nutritional Engineering China Agricultural University Beijing100083 China

Pea seedlings (Pisum sativum L.) were used as materials to test the timings and compartments of hydrogen peroxide (H2O2) triggered by wounding and exogenous jasmonic acid (JA). The results showed that H2O2 could be systemically induced by wounding and exogenous JA. H2O2 increased within 1 h and reached the peak 3―5 h after wounding in either the wounded leaves or the unwounded leaves adjacent to the wounded ones and the inferior leaves far from the wounded ones. After this, H2O2 decreased and recovered to the control level 12 h after wounding. The activities of antioxidant enzymes, however, were rapidly increased by wounding. Diphenylene iodonium (DPI), an inhibitor of NADPH oxidase, could significantly inhibit H2O2 burst that was mediated by wounding and exogenous JA. Assay of H2O2 subcellular location showed that H2O2 in response to wounding and exogenous JA was predominantly accumulated in plasma membrane, cell wall and apoplasmic space. Numerous JA (gold particles) was found via immu- nogold electron microscopy to be located in cell wall and phloem zones of mesophyll cell after wounding.

关键词： wounding, jasmonic acid, H2O2, histochemical location, cytochemical location, immunogold electron microscopy location.

Effect of jasmonic Acid on Photosynthetic Pigments and Stress Markers in <i>Cajanus cajan</i>(L.) Millsp. Seedlings under Copper Stress

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American Journal of Plant Sciences 2013年第4期4卷 817-823页

作者： Sharma Poonam Harpreet Kaur Sirhindi Geetika Department of Botany Punjabi University Patiala India Department of Botany Punjabi University Patiala India.

Jasmonates are class of plant growth regulators act as signal molecule that intercede various components in physiological and metabolic regulation, stress responses and possibly communication through signal transduction. Oxidative stress due to heavy metal exposure stimulates synthesis and activity of antioxidant metabolites and enhances antioxidant enzyme activities that could protect plant tissues. The aim of this study was to investigate the exogenous effect of JA at seed level which can transduce throughout seedling growth and regulate antioxidant activities such as superoxide dismutase (SOD;EC 1.***.1.1) and guaiacol peroxidase (POD;EC 1.***.1.7) in 12 days old seedlings of pigeon pea (Cajanus cajan (L.) Millsp.) in presence and/or absence of copper. The activity of SOD and POD increased significantly in presence of Cu2+ after seed priming with JA. JA also helps in chlorophyll and carotenoid accumulation and neutralizes the toxic effect of Cu2+ on seedlings. This is the first report of JA effect on photosynthetic pigment accumulation and H2O2 mitigating enzymes i.e. SOD and POD and it could be recommended that seed priming with JA help in ameliorating toxic effect of Cu2+.

关键词： jasmonic Acid Copper Sulphate Lipid Peroxidation Superoxide Dismutase Guaiacol Peroxidase Total Chlorophyll Carotenoids

Nitric oxide mediates the fungal elicitor-induced puerarin biosynthesis in Pueraria thomsonii Benth.suspension cells through a salicylic acid(SA)-dependent and a jasmonic acid(JA)-dependent signal pathway

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Science China(Life Sciences) 2006年第4期49卷 379-389页

作者： XU Maojun1, DONG Jufang1 & ZHU Muyuan2 Department of Biotechnology, Zhejiang Gongshang University, Hangzhou 310035, China State Key Laboratory of Plant Physiology and Biochemistry, College of Life Sciences, Zhejiang University, Hangzhou 310012, China Department of Biotechnology Zhejiang Gongshang University Hangzhou 310035 China State Key Laboratory of Plant Physiology and Biochemistry College of Life Sciences Zhejiang University Hangzhou 310012 China

Nitric oxide (NO) has emerged as a key signaling molecule in plant secondary metabolite biosynthesis recently. In order to investigate the molecular basis of NO signaling in elicitor-induced secondary metabolite biosynthesis of plant cells, we determined the contents of NO, salicylic acid (SA), jasmonic acid (JA), and puerarin in Pueraria thomsonii Benth. suspension cells treated with the elicitors prepared from cell walls of Penicillium citrinum. The results showed that the fungal elicitor induced NO burst, SA accumulation and puerarin production of P. thomsonii Benth. cells. The elicitor-induced SA accumulation and puerarin production was suppressed by nitric oxide specific scavenger cPITO, indicating that NO was essential for elicitor-induced SA and puerarin biosynthesis in P. thomsonii Benth. cells. In transgenic NahG P. thomsonii Benth. cells, the fungal elicitor also induced puerarin biosynthesis, NO burst, and JA accumulation, though the SA biosynthe-sis was impaired. The elicitor-induced JA accumulation in transgenic cells was blocked by cPITO, which suggested that JA acted downstream of NO and its biosynthesis was controlled by NO. External application of NO via its donor sodium nitroprusside (SNP) enhanced puerarin biosynthesis in trans-genic NahG P. thomsonii Benth. cells, and the NO-triggered puerarin biosynthesis was suppressed by JA inhibitors IBU and NDGA, which indicated that NO induced puerarin production through a JA-dependent signal pathway in the transgenic cells. Exogenous application of SA suppressed the elicitor-induced JA biosynthesis and reversed the inhibition of IBU and NDGA on elicitor-induced pu-erarin accumulation in transgenic cells, which indicated that SA inhibited JA biosynthesis in the cells and that SA might be used as a substitute for JA to mediate the elicitor- and NO-induced puerarin biosynthesis. It was, therefore, concluded that NO might mediate the elicitor-induced puerarin bio-synthesis through SA- and JA-dependent signal pathways in wildtype P. thomsonii Benth. cells an

关键词： nitric oxide (NO), salicylic acid (SA), jasmonic acid (JA), fungal elicitor, puerarin.

Changes in the Production of Salicylic and jasmonic Acid in Potato Plants (<i>Solanum tuberosum</i>) as Response to Foliar Application of Biotic and Abiotic Inductors

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American Journal of Plant Sciences 2015年第11期6卷 1785-1791页

作者： Esmeralda González-Gallegos Elan Laredo-Alcalá Juan Ascacio-Valdés Diana Jasso de Rodríguez Francisco Daniel Hernández-Castillo Departamento de Parasitología Agrícola Universidad Autónoma Agraria Antonio Narro Saltillo México

An alternative to the use of chemical fungicides is to enhance the defensive response of plants by appropriate stimulation, a phenomenon known as induction of resistance. The aim of this study was to determine the changes of endogen levels of salicylic acid (SA) and jasmonic acid (JA) in potato plants as response to foliar application of biotic and abiotic inductors. Treatments T1 = Best Ultra F (Bacillus spp. 108 cfu/mL and Pseudomonas fluorescens 108 cfu/mL) 0.5%, T2 = FullKover HF (microbial jasmonic acid 1500 ppm) 0.2%, T3 = T1 0.5% + T2 0.1%, T4 = Milor® (Chlorothalonil + Metalaxyl) 0.5% and T5 = control (water) were applied in potato plants. The application of biotic and abiotic inductors improved the SA and JA production in potato plants. The production of salicylic acid in potato plants was observed by application of Bacillus spp. and Pseudomonas fluorescens (T1) and fungicide Milor® (T4). The application of T1 Best Ultra F, T2 FullKover HF (microbial JA), T3 (T1 + T2) and T4 Milor® improved the JA production in potato plants.

关键词： Salicylic Acid jasmonic Acid Bacillus spp. Pseudomonas fluorescens

Reduced grain chalkiness and its possible physiological mechanism in transgenic rice overexpressing L-GalLDH

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The Crop Journal 2015年第2期3卷 125-134页

作者： Le Yu Yonghai Liu Jianhua Tong Junhui Ding Ruozhong Wang Changlian Peng Langtao Xiao College of Life Sciences Zhaoqing University Hunan Provincial Key Laboratory of Phytohormones and Growth Development Hunan Agricultural University College of Life Sciences South China Normal University

Chalkiness is one of the key factors determining rice quality and price. Ascorbic acid(Asc) is a major plant antioxidant that performs many functions in plants. L-Galactono-1,4-lactone dehydrogenase(L-Gal LDH, EC1.***.2.3) is an enzyme that catalyzes the final step of Asc biosynthesis in plants. Here we show that the L-Gal LDH-overexpressing transgenic rice, GO-2,which has constitutively higher leaf Asc content than wild-type(WT) plants, exhibits significantly reduced grain chalkiness. Higher foliar ascorbate/dehydroascorbate(Asc/DHA)ratios at 40, 60, 80, and 100 days of plant age were observed in GO-2. Further investigation showed that the enhanced level of Asc resulted in a significantly higher ribulose-1,5-bisphosphate(Ru BP) carboxylase/oxygenase(Rubisco) protein level in GO-2 at 80 days. In addition, levels of abscisic acid(ABA) and jasmonic acid(JA) were lower in GO-2 at 60, 80, and100 days. The results we present here indicate that the enhanced level of Asc is likely responsible for changing redox homeostasis in key developmental stages associated with grain filling and alters grain chalkiness in the L-Gal LDH-overexpressing transgenic by maintaining photosynthetic function and affecting phytohormones associated with grain filling.

关键词： Grain chalkiness Ascorbic acid Abscisic acid jasmonic acid Rice

Transcriptome analysis of salt-stress response in three seedling tissues of common wheat

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The Crop Journal 2019年第3期7卷 378-392页

作者： Qiaoling Luo Wan Teng Shuang Fang Hongwei Li Bin Li Jinfang Chu Zhensheng Li Qi Zheng State Key Laboratory of Plant Cell and Chromosome Engineering Institute of Genetics and Developmental BiologyChinese Academy of SciencesBeijing 100101China University of Chinese Academy of Sciences Beijing 100049China National Centre for Plant Gene Research (Beijing) Institute of Genetics and Developmental BiologyChinese Academy of SciencesBeijing 100101China

Xiaoyan 60(XY60) is a new wheat variety bred in the laboratory of Zhensheng Li.After salt treatment, seedlings of XY60 maintain green leaves and produce longer roots than the high yielding cultivar Zhongmai 175(ZM175).To explain these different phenotypes we carried out an RNA-Seq analysis using 12 samples from three tissues of both varieties subjected to salt and control treatments.By comparing data from the salt treated plants with the control, 703, 979, and 1197 differentially expressed genes(DEGs) were detected in new leaves, old leaves, and roots of XY60, respectively.The corresponding numbers for ZM175 were 613, 1401, and 1301.The most significantly enriched Gene Ontology(GO) terms and KEGG pathways were associated with polyunsaturated fatty acid(PUFA) metabolism in both new and old leaves from XY60.They were associated with photosynthesis and energy metabolism in ZM175.The most significantly enriched KEGG pathway in roots of both varieties was “glucosinolate biosynthesis”.In addition, jasmonic acid(JA) concentration in XY60 was higher than in ZM175, although it increased significantly in both varieties following salt treatment.Trends in relative expression levels of AOS, MYC2, and JAZ revealed by qRT-PCR were concordant with those from RNA-Seq.Our results suggest that PUFAs may contribute to salt tolerance in common wheat by enhancing the photosynthetic system and JA-related pathways.

关键词： jasmonic acid Photosynthesis RNA-Seq Salt tolerance Triticum aestivum

Expression Analysis of Genes Related to Rice Resistance Against Brown Planthopper, Nilaparvata lugens

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Rice science 2017年第3期24卷 163-172页

作者： Panatda JANNOEY Duangdao CHANNEI Jate KOTCHARERK Weerathep PONGPRASERT Mika NOMURA Department of Biochemistry Faculty of Medical Science Naresuan University Pitsanulok 65000 Thailand

Brown planthopper(BPH) is an insect species that feeds on the vascular system of rice plants. To examine the defence mechanism of rice plants against BPH, the pathogenesis-related genes(PR1a, PR2, PR3, PR4, PR6, PR9, PR10a, PR13, PR15 and PRpha), signaling molecule synthesis genes(AOS, AXR, ACO and LOX), antioxidant-related genes(CAT, TRX, GST and SOD) and lignin biosynthesis-related genes(CHS, CHI and C4H) were investigated in a resistant rice variety. AOS, PR6,PR9 and PR15 genes showed significantly increased relative expression levels at 24.38-, 19.17-, 14.71-, and 12.74-fold compared to the control. Moderate increased relative expression levels of lignin biosynthesis-related gene(C4H), pathogenesis-related genes(PR4, PR10a and PRpha), and antioxidant-related gene(GST) were found, while CHI, LOX, SOD, TRX1 and AXR showed decreased relative expression levels. It was thus clearly shown that wound-induced response genes were activated in rice plants after BPH attacks through AOS activation. jasmonic acid signaling molecule may activate PR6, PR15, GST and CAT subsequently increasing their expression for H_2O_2 detoxification. PR6 were expressed at the highest relative level among the PR genes. These genes therefore have also a considerable synergistic role with the other genes against BPH by interfered their digestion tract system.

关键词： brown planthopper rice defense mechanism gene expression jasmonic acid

Integrated transcriptomics and metabolomics analyses provide insights into cold stress response in wheat

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The Crop Journal 2019年第6期7卷 857-866页

作者： Yong Zhao Meng Zhou Ke Xu Jiahao Li Shanshan Li Shuhua Zhang Xueju Yang Huabei Key Laboratory of Crop Germplasm Hebei Agricultural UniversityBaoding 071000HebeiChina College of Life Sciences Hebei UniversityBaoding 071000HebeiChina

Cold tolerance of crop plants influences survival and productivity under low-temperature conditions. Elucidation of molecular mechanisms underlying low temperature tolerance could be helpful in breeding. In this study, we used integrated transcriptomics and metabolomics analyses to investigate changes in gene/metabolite activity in a winter-hardy wheat cultivar of(cv. Jing 411) when subjected to sold stress. The 223 metabolites mainly enriched during cold acclimation included carbohydrates, flavonoids, and amino acids.Eight common metabolites had altered abundance following freezing treatment;six increased and two decreased. Transcriptome analysis revealed that 29,066 genes were differentially expressed in wheat crowns after cold acclimation compared to the nonacclimated control. Among them, 745 genes were up-regulated following freezing treatment, suggesting substantial change in expression of a large quantity of genes upon cold acclimation and freezing treatment, which impacts on the modified metabolites.Integrated analysis of gene expression and metabolite profiles revealed that the abscisic acid(ABA)/jasmonic acid(JA) phytohormone signaling and proline biosynthesis pathways were significantly modulated under cold acclimation and freezing treatments. Our results indicated that low-temperature stress induced substantial changes in both transcriptomes and metabolomes. Critical pathways associated with ABA/JA signaling and proline biosynthesis played important roles in regulating cold tolerance in wheat.

关键词： Cold tolerance Transcriptome Metabolome Abscisic acid jasmonic acid Signaling pathways