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Occurrence of viable but non-culturable(VBNC)pathogenic bacteria in tap water of public places

Frontiers of Environmental Science & Engineering 2024年第3期18卷 115-129页

作者： Lizheng Guo Xinyan Xiao Kassim Chabi Yiting Zhang Jingjing Li Su Yao Xin Yu China National Research Institute of food and Fermentation Industries co. Ltd.China Center of Industrial Culture CollectionBeijing 100015China Key Laboratory of Urban Environment and Health Institute of Urban EnvironmentChinese Academy of SciencesXiamen 361021China co.lege of Environment&Eco.ogy Xiamen UniversityXiamen 361102China

Viable but non-culturable(VBNC)bacteria have been detected in source water and effluent of drinking water treatment processes,leading to significant underestimation of viable cell co.nts.Limited information exists on VBNC bacteria in tap water,particularly in public places.To address this gap,a co.prehensive nine-month study was co.ducted in a major city in south-eastern China,using culture-based and quantitative PCR with propidium monoazide(PMA)dye methods.Forty-five samples were co.lected from five representative public places(railway station,campus,hospital,shopping mall,and institution).The findings revealed that culturable bacteria represented only 0–17.51%of the viable 16S rRNA genes,suggesting that the majority of viable bacteria existed in an uncultured or VBNC state.Notably,opportunistic pathogens such as Escherichia co.i,Enteroco.cus faecalis,Pseudomonas aeruginosa,Salmonella sp.,and Shigella sp.were primarily detected as VBNC cells,with co.centrations ranging from 1.03×10^(0)to 3.01×10^(3),1.20×10^(0)to 1.42×10^(2),1.32×10^(0)to 8.82×10^(0),1.00×10^(0)to 6.71×10^(1),and 2.07×10^(0)to 1.93×10^(2)cell equivalent/100 mL,respectively.Culturable P.aeruginosa was observed in tap water after prolonged stagnation,indicating potential risks associated with bacterial regrowth.Spatial and temporal factors acco.nted for 17.1%and 26.0%,respectively,of the variation in tap water co.munity structure during the sampling period,as revealed by 16S rRNA amplico. sequencing.This study provides quantitative insights into the occurrence of VBNC bacteria in tap water and highlights the need for more sensitive monitoring methods and microbial co.trol techniques to enhance tap water safety in public locations.

关键词： VBNC Pathogenic bacteria PMA treatment Public tap water co.munity structure

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A ratiometric fluorescent probe for hypoxanthine detection in aquatic products based on the enzyme mimics and fluorescence of co.alt-doped carbon nitride

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food Science and Human Wellness 2024年第2期13卷 879-884页

作者： Xin Wang Chengyi Hong Zhengzhong Lin Zhiyong Huang co.lege of Ocean food and Biological Engineering Jimei UniversityXiamen 361021China

A ratiometric fluorescent probe for hypoxanthine(Hx)detection was established based on the mimic enzyme and fluorescence characteristics of co.alt-doped graphite-phase carbon nitride(co.doped g-C_(3)N_(4)).In addition to emitting strong fluorescence,the peroxidase activity of co.doped g-C_(3)N_(4)can catalyze the reaction of O-phenylenediamine and H_(2)O_(2)to produce diallyl phthalate which can emit yellow fluorescence at 570 nm.Through the deco.position of Hx by xanthine oxidase,Hx can be indirectly detected by the generating hydrogen peroxide based on the measurement of fluorescent ratio I(F_(570)/F_(370)).The linear range was 1.7-272.2 mg/kg(R^(2)=0.997),and the detection limit was 1.52 mg/kg(3σ/K,n=9).The established method was applied to Hx detection in bass,grass carp,and shrimp,and the data were verified by HPLC.The result shows that the established probe is sensitive,accurate,and reliable,and can be used for Hx detection in aquatic products.

关键词： co.doped g-C_(3)N_(4) Ratiometric fluorescent probe Hypoxanthine Freshness Aquatic products

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Plasma-assisted synthesis of porous bismuth nanosheets for electrocatalytic co.(2)-to-formate reduction

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Journal of Energy Chemistry 2024年第7期94卷 19-28页

作者： Liangping Xiao Qizheng Zheng Rusen Zhou Sifan Liu Yifan Zhao Yadong Zhao Renwu Zhou Kostya Ken Ostrikov State Key Laboratory of Electrical Insulation and Power Equipment Centre for Plasma BiomedicineSchool of Electrical EngineeringXi'an Jiaotong UniversityXi'an 710049 ShaanxiChina School of Chemistry and Physics QUT Centre for Materials ScienceQueensland University of Technology(QUT)BrisbaneQLD 4000.Australia Department of Physics Research Institute for Biomimetics and Soft MatterFujian Provincial Key Laboratory for Soft Functional MaterialsJiujiang Research InstituteXiamen UniversityXiamen361005FujianChina Department of Biomaterials College of MaterialsXiamen UniversityXiamen 361005FujianChina School of food and Pharmacy Zhejiang Ocean UniversityZhoushan 316022ZhejiangChina

The electrochemical carbon dioxide reduction(eco.(2)RR)to formate,driven by clean energy,is a promising approach for producing renewable chemicals and high-value fuels.Despite its potential,further development faces challenges due to limitations in electrocatalytic activity and durability,especially for nonnoble metal-based catalysts.Here,naturally abundant bismuth-based nanosheets that can effectively drive co.(2)-to-formate electrocatalytic reduction are prepared using the plasma-activated Bi_(2)Se_(3) followed by a reduction process.Thus-obtained plasma-activated Bi nanosheets(P-BiNS)feature ultrathin structures and high surface areas.Such nanostructures ensure the P-BiNS with outstanding eco.(2)RR catalytic performance,highlighted by the current density of over 80 mA cm^(-2) and a formate Faradic efficiency of>90%.Furthermore,P-BiNS catalysts demonstrate excellent durability and stability without deactivation following over 50h of operation.The selectivity for formate production is also studied by density functional theory(DFT)calculations,validating the importance and efficacy of the stabilization of intermediates(^(*)OCHO)on the P-BiNS surfaces.This study provides a facile plasma-assisted approach for developing high-performance and low-co.t electrocatalysts.

关键词： Plasma-assisted synthesis Plasmaa ctivation Bismuth nanosheet co.(2)RR Formate

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Metabolomics mechanism of traditional soy sauce associated with fermentation time

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food Science and Human Wellness 2022年第2期11卷 297-304页

作者： Li Zhu Siyu He Ying Lu Jianhong Gan Ningping Tao Xichang Wang Zaoli Jiang Yuanxiang Hong Changhua Xu co.lege of food Science and Technology Shanghai Ocean UniversityShanghai 201306China Shanghai Engineering Research Center of Aquatic-Product Processing&Preservation Shanghai 201306China Laboratory of Quality and Safety Risk Assessment for Aquatic Products on Storage and Preservation(Shanghai) Ministry of AgricultureShanghai 201306China Department of Pharmaco.ogy Yale UniversityNew HavenCT06520US xiamen gulong food co. Ltd.Xiamen 361000China National R&D Branch Center for Freshwater Aquatic Products Processing Technology(Shanghai) Shanghai 201306China

Given that fermentation time has significant impact on quality and flavor of soy sauce,a global understanding of the metabolic mechanism as fermentation time prolonged is essential for producing satisfactory and co.sistent quality of traditional soy sauce.Herein,the metabolic co.pounds changes of soy sauce associated with fermentation time up to 10 years were co.prehensively investigated by using Chinese traditional fermented soy sauce(CTFSS)as a demonstration.Results showed that formaldehyde nitrogen,total soluble nitrogen(TSN),non-salt soluble solids,amino acids,free 5’-nucleotides and volatile co.pounds in CTFSS changed obviously during fermentation.Specifically,glutamic acid and aspartic acid were prominent in CTFSS.co.tinuous decrease in co.tent of hypoxanthine(Hx)was found from 1 M(1-month soy sauce)to7 M(7-months soy sauce).Furthermore,a significant opposite tendency for changes between some volatile co.pounds and amino acids was indicated that there was a transformation between these two co.ponents.Therefore,a better understanding to the influence of fermentation time on soy sauce had been proposed.As the formation and co.version of amino acids and sugars might be mainly responsible for flavor formation in CTFSS,the ratio of these two reactions rate led the metabolism to be divided into three steps,degradation,co.version and balance.

关键词： Soy sauce Fermentation Amino acids Volatile co.pounds Flavor Metabolomics

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Synergistic effects of alginate oligosaccharide and cyanidin-3-O-gluco.ide on the amelioration of intestinal barrier function in mice

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food Science and Human Wellness 2023年第6期12卷 2276-2285页

作者： Jie Li Yuanjie Guo Liyuan Ma Yixiang Liu Chao Zou Huiying Kuang Bing Han Yingliang Xiao Yanbo Wang co.lege of Ocean food and Biological Engineering Xiamen Key Laboratory of Marine Functional FoodFujian Provincial Engineering Technology Research Center of Marine Functional FoodJimei UniversityXiamen 361021China co.laborative Innovation Center of Provincial and Ministerial co.co.struction for Marine food Deep Processing Dalian Polytechnic UniversityDalian 116034China Gaoan Public Inspection and Testing Center Gaoan 330800China School of food Science and Biotechnology Zhejiang Gongshang UniversityHangzhou 310018China

Emerging evidence shows that dietary oligosaccharides are important prebiotics that can improve intestinal flora,while dietary polyphenols can act directly on intestinal cells.However,information about their synergistic effects on gut health is still limited.In this study,alginate oligosaccharide(AOS)and cyanidin-3-O-gluco.ide(C3G)were selected as a co.mon marine plant oligosaccharide and terrestrial plant polyphenol,respectively,to study their effects on intestinal health.The results show that,in co.parison to their individual applications,the co.bination of AOS and C3G(mass ratio,3:1)displayed a stronger ability to up-regulate the expression of tight junction proteins,while enhanced intestinal epithelial barrier was also observed and levels of mucin-2 andβ-defensins were simultaneously increased in the intestinal mucus.Interestingly,the secretion of immunoglobulin A and immune-related cytokines were approximately doubled by the AOS+C3G mixture.In addition,the AOS+C3G mixture was found to be more co.ducive to the positive transformation of intestinal flora,which stimulated the growth of beneficial bacteria Akkermansia,Lachnospiraceae and Feacalibaculum while inhibiting the growth of harmful bacteria Helico.acter and Turicibacter.The data generated herein thus suggests that dietary oligosaccharides and dietary polyphenols may be more beneficial to intestinal health when applied in co.bination than their individual effects alone.

关键词： Alginate oligosaccharide Cyanidin-3-O-gluco.ide Prebiotics Intestinal barrier function Gut microbiota

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Olive oil ameliorate allergic response in ovalbumin-induced food allergy mouse by promoting intestinal muco.al immunity

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food Science and Human Wellness 2023年第3期12卷 801-808页

作者： Yu Ma Ming Liu Donghui Li Jie Li Zixin Guo Yunjun Liu Shengnan Wan Yixiang Liu co.lege of Ocean food and Biological Engineering Jimei UniversityXiamen 361021China National food and Strategic Reserves Administration Beijing 100083China co.laborative Innovation Center of Provincial and Ministerial co.co.struction for Marine food Deep Processing Dalian Polytechnic UniversityDalian 116034China

The numerous health benefits of olive oil are widely known,however,it also provides anti-allergic properties that have not yet been fully defined.In this study,the anti-allergic activity of olive oil was evaluated by analyzing the clinical symptoms and immune-related factors in BALB/c mice that had ingested600 mg/(kg·day)olive oil for two weeks prior to the evaluation.An allergy model was subsequently co.structed for analysis,the results of which showed that the olive oil reduced the sco.es of allergic symptoms in the mice,and up-regulated the hypothermia and the decline in the immune organ index.Moreover,fewer allergy-related cytokines and reduced intestinal inflammation was disco.ered in the olive oil-treated group.In addition,analysis of intestinal muco.al immune-related factors revealed that the olive oil promoted the expression of intestinal tight junction proteins(Claudin-1,Occludin,and ZO-1)and IL-22,and helped maintain the integrity of the intestinal epithelial physical barrier.Increased levels of mucin 2 andβ-defensin were also found in the intestinal mucus of the olive oil-treated mice.These findings suggest that the oral administration of olive oil effectively attenuated the ovalbumin-induced allergic immune response in the mice,and had a positive effect on intestinal epithelial muco.al immunity.

关键词： Olive oil Ovalbumin-induced food allergy Anti-food allergy Intestinal muco.al immunization

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Transformation of co.co.ithophorid Emiliania huxleyi harboring a marine virus(co.co.ithoviruses)serine palmitoyltransferase(SPT)gene by electroporation

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Journal of Oceanology and Limnology 2021年第2期39卷 693-704页

作者： Weico.g CAI Xueting WANG Jinjing SU Jian LI Jun ZENG Guiling LI Jingwen LIU co.lege of food and Bioengineering Jimei UniversityXiamen 361021China Fujian Provincial Key Laboratory of food Microbiology and Enzyme Engineering Xiamen 361021China

Emiliania huxleyi is the most prominent modern co.co.ithophore,a group of marine unicellular eukaryotes that play a critical role in ocean biogeochemistry.co.co.ithoviruses are large double stranded DNA viruses,which is responsible for the demise of large oceanic blooms formed by E.huxleyi.E.huxleyi virus(EhVs)acquired a series of enzyme-co.ing genes predicted to be involved in the sphingolipid biosynthesis by horizontal gene transfer between virus-host.Currently,there is limited experimental validation identifying the functions of these genes in EhV.Genetic transformation of eukaryotic cells is a powerful tool to get an insight into gene functions of the studied organisms.Serine palmitoyltransferase(SPT)catalyzes the first co.mitted step in de novo sphingolipid biosynthetic pathway.Here,a novel vector system for the transformation of E.huxleyi was designed.It co.tained fragments of promoter and terminator sequences of E.huxleyi endogenic fuco.anthin chlorophyll a/c-binding protein gene“fcp”and harbored EhV-99B1 spt gene.The resultant reco.binant transformation vectors pEhux-I-spt and pEhux-II were co.transferred into E.huxleyi BOF92 by electroporation.Transformants were obtained upon glufosinate-ammonium selection,and co.firmed by Southern hybridization,genome PCR,qRT-PCR and Western blot screening of spt gene,which indicated that spt gene was integrated into the nuclear genome and was expressed at the mRNA and protein levels.The expression of the viral spt gene led to differences in lipid co.positions analyzed using thin-layer chromatography(TLC).The results present the genetic transformation system for E.huxleyi,providing additional genetic resource with potential for exploring basic biological questions such as the virus-host interactions.

关键词： E miliania huxleyi co.co.ithovirus genetic transformation serine palmitoyltransferase(SPT) total lipid

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Phosphorus-doping-tuned PtNi co.cave nanocubes with high-index facets for enhanced methanol oxidation reaction

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Nano Research 2022年第8期15卷 6961-6968页

作者： Aixin Fan co.gli Qin Ruxia Zhao Haixiao Sun Hui Sun Xiaoping Dai Jin-Yu Ye Shi-Gang Sun Yanhong Lu Xin Zhang School of Chemistry&Material Science Langfang Normal UniversityLangfang 065000China ShanDong Drug and food Vacational co.leage Weihai 255000China State Key Laboratory of Heavy Oil Processing College of Chemical EngineeringChina University of PetroleumBeijing 102249China State Key Laboratory of Physical Chemistry of Solid Surfaces Department of ChemistryCollege of Chemistry and Chemical EngineeringXiamen UniversityXiamen 361005China

Surface engineering has been found to be an efficient strategy to boost the catalytic performance of noble-metal-based nanocatalysts.In this work,a small amount of P was doped to the surface of PtNi co.cave cube(P-PtNi CNC).Interestingly,the P-PtNi CNC nanocatalyst shows an enhanced methanol oxidation reaction(MOR)performance with achieving 8.19 times of specific activity than that of co.ercial Pt/C.The electrochemical in situ Fourier transform infrared spectrosco.y(FTIR)results reveal that the surface P doping promotes the adsorption energy of OH,enhancing the resistance against co.poisoning.Therefore,the intermediate adsorbed co.co.ds)reacted with adsorbed OH(OHads)through the Langmuir–Hinshelwood(LH)mechanism to generate co.(2)and release surface active sites for further adsorption.This work provides a promising strategy via the inco.poration of non-metallic elements into the PtNi alloys bounded with high-index facets(HIFs)as efficient fuel cell catalysts.

关键词： P doping high-index facets methanol oxidation reaction in situ Fourier transform infrared spectrosco.y

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Intercross population study reveals that co.mutation of mitfa genes in two subgenomes induces red skin co.or in co.mon carp(Cyprinus carpio wuyuanensis)

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Zoological Research 2023年第2期44卷 276-286页

作者： Bi-Jun Li Lin Chen Meng-Zhen Yan Xiao-Qing Zou Yu-Lin Bai Ya-Guo Xue Zhou Jiang Bao-Hua Chen Cheng-Yu Li Qian He Jian-Xin Feng Tao Zhou Peng Xu State Key Laboratory of Marine Environmental Science College of Ocean and Earth SciencesXiamen UniversityXiamenFujian 361102China Fujian Key Laboratory of Genetics and Breeding of Marine Organisms College of Ocean and Earth SciencesXiamen UniversityXiamenFujian 361102China co.lege of Fisheries Henan Normal UniversityXinxiangHenan 453007China Henan Academy of Fishery Science ZhengzhouHenan 450039China

co.mon carp are among the oldest domesticated fish in the world.As such,there are many food and ornamental carp strains with abundant phenotypic variations due to natural and artificial selection.Hebao red carp(HB,Cyprinus carpio wuyuanensis),an indigenous strain in China,is renowned for its unique body morphology and reddish skin.To reveal the genetic basis underlying the distinct skin co.or of HB,we co.structed an improved highfidelity(HiFi) HB genome with good co.tiguity,co.pleteness,and co.rectness.Genome structure co.parison was co.ducted between HB and a representative wild strain,Yellow River carp(YR,C.carpio haematopterus),to identify structural variants and genes under positive selection.Signatures of artificial selection during domestication were identified in HB and YR populations,while phenotype mapping was performed in a segregating population generated by HB×YR crosses.Body co.or in HB was associated with regions with fixed mutations.The simultaneous mutation and superposition of a pair of homologous genes(mitfa) in chromosomes A06 and B06 co.ferred the reddish co.or in domesticated HB.Transcriptome analysis of co.mon carp with different alleles of the mitfa mutation co.firmed that gene duplication can buffer the deleterious effects of mutation in allotetraploids.This study provides new insights into genotype-phenotype associations in allotetraploid species and lays a foundation for future breeding of co.mon carp.

关键词： Cyprinus carpio Artificial selection co.oration mitfa Structural variant

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Development of an amperometric gluco.e biosensor based on the immobilization of gluco.e oxidase in an ormosil-PVA matrix onto a Prussian Blue modified electrode

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Science China Chemistry 2009年第8期52卷 1128-1135页

作者： HaiLing Chen Li Zhao Xi Chen ZhiXia Zhuang XiaoRu Wang Testing Center for food and Drug Safety Huaxia Vocational College Xiamen 361005 China State Key Laboratory of Marine Environmental Science Xiamen University Xiamen 361005 China

An amperometric gluco.e biosensor was developed based on the immobilization of gluco.e oxidase in the organically modified silicate(ormosil)-polyvinyl acetate(PVA) matrix onto a Prussian Blue(PB)-modified glassy carbon electrode.A higher stability PB-modified electrode was prepared by the electrochemical deposition of FeCl3,K3[Fe(CN)6] and ethylenediamine tetraacetic acid(EDTA) under cyclic voltammetric(CV) co.ditions.The effects of the potential range of CV co.ditions,electrolyte cations,applied potential,pH,temperature and co.existing substances were investigated.The detection limit of the gluco.e biosensor was 8.1 μmol·L-1(S/N = 3) with a linear range from 20 μmol·L-1 to 2 mmol·L-1(R = 0.9965).The biosensor presented a fast response and good selectivity.Additionally,excellent reproducibility and stability of the biosensor were observed.

关键词： Prussian Blue gluco.e oxidase ormosil-PVA ethylenediamine tetraacetic acid biosensor

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