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Resolving the developmental mechanisms of cardiac microthrombosis of SARS-CoV-2 based on single-cell transcriptome analysis

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Science China Life Sciences 2025年第1期 103-115页

作者： xizi Luo Nan Zhang Yuntao Liu Beibei Du Xuan Wang Tianxu Zhao Bingqiang Liu Shishun Zhao Jiazhang Qiu Guoqing Wang State key laboratory for Diagnosis and treatment of Severe Zoonotic Infectious diseases Key Laboratory for Zoonosis Research of the Ministry of EducationCollege of Basic MedicineJilin University State key laboratory for Diagnosis and treatment of Severe Zoonotic Infectious diseases Key Laboratory for Zoonosis Research of the Ministry of EducationCollege of Veterinary MedicineJilin University College of Mathematics Jilin University Bioinformatic Lab School of MathematicsShandong University Department of Cardiology China-Japan Union HospitalJilin University

The coronavirus disease 2019（COVID-19） outbreak caused by the severe acute respiratory syndrome coronavirus-2（SARS-CoV-2） developed into a global health emergency. Systemic microthrombus caused by SARS-CoV-2 infection is a common complication in patients with COVID-19. Cardiac microthrombosis as a complication of SARS-CoV-2 infection is the primary cause of cardiac injury and death in patietns with severe COVID-19. In this study, we performed single-cell sequencing analysis of the right ventricular free wall tissue from healthy donors,patients who died during the hypercoagulable period of characteristic coagulation abnormality（CAC）, and patients who died during the fibrinolytic period of CAC. We collected 61,187 cells enriched in 24 immune cell subsets and 13 cardiac-resident cell subsets. We found that in the course of SARS-CoV-2 infected heart microthrombus, MYO1EhighRASGEF1Bhighmonocyte-derived macrophages promoted hyperactivation of the immune system and initiated the extrinsic coagulation pathway by activating chemokines CCL3, CCL5. This series of events is the main cause of cardiac microthrombi following SARS-CoV-2 infection. In a SARS-CoV-2 infected heart microthrombus, excessive immune activation is accompanied by an increase in cellular iron content, which in turn promotes oxidative stress and intensifies intercellular competition. This induces cells to alter their metabolic environment, resulting in increased sugar uptake via the glycosaminoglycan synthesis pathway. In addition, high levels of reactive oxygen species generated by elevated iron levels promote increased endogenous malondialdehyde synthesis in a subpopulation of cardiac endothelial cells. This exacerbates endothelial cell dysfunction and exacerbates the coagulopathy process.

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Tranylcypromine upregulates Sestrin 2 expression to ameliorate NLRP3-related noise-induced hearing loss

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Neural Regeneration Research 2025年第5期20卷 1483-1494页

作者： xihang Chen Zhifeng Chen Menghua Li Weiwei Guo Shuolong Yuan Liangwei Xu Chang Lin xi Shi Wei Chen Shiming Yang Senior Department of Otolaryngology Head and Neck Surgery Chinese PLA General HospitalChinese PLA Medical SchoolBeijingChina State key laboratory of Hearing and Balance Science BeijingChina National Clinical Research Center for Otolaryngologic diseases BeijingChina key laboratory of Hearing Science Ministry of EducationBeijingChina Beijing key laboratory of Hearing Impairment prevention and treatment BeijingChina Department of Otorhinolaryngology Head and Neck Surgery The First Affiliated Hospital of Fujian Medical UniversityFuzhouFujian ProvinceChina Department of Otolaryngology Head and Neck Surgery The 940th Hospital of Joint Logistics Support Force of Chinese PLALanzhouGansu ProvinceChina key laboratory of Tropical Biological Resources of Ministry of Education School of Pharmaceutical SciencesHainan UniversityHaikouHainan ProvinceChina Academician Workstation of Hainan University School of Pharmaceutical SciencesYazhou BaySanyaHainan ProvinceChina

Noise-induced hearing loss is the primary non-genetic factor contributing to auditory ***,there are currently no effective pharmacological interventions for patients with noise-induced hearing ***,we present evidence suggesting that the lysine-specific demethylase 1 inhibitor–tranylcypromine is an otoprotective agent that could be used to treat noise-induced hearing loss,and elucidate its underlying regulatory *** established a mouse model of permanent threshold shift hearing loss by exposing the mice to white broadband noise at a sound pressure level of 120 d B for 4 *** found that tranylcypromine treatment led to the upregulation of Sestrin2(SESN2)and activation of the autophagy markers light chain 3B and lysosome-associated membrane glycoprotein 1 in the cochleae of mice treated with *** noise exposure group treated with tranylcypromine showed significantly lower average auditory brainstem response hearing thresholds at click,4,8,and 16 k Hz frequencies compared with the noise exposure group treated with *** findings indicate that tranylcypromine treatment resulted in increased SESN2,light chain 3B,and lysosome-associated membrane glycoprotein 1 expression after noise exposure,leading to a reduction in levels of 4-hydroxynonenal and cleaved caspase-3,thereby reducing noise-induced hair cell ***,immunoblot analysis demonstrated that treatment with tranylcypromine upregulated SESN2 expression via the autophagy *** treatment also reduced the production of NOD-like receptor family pyrin domaincontaining 3(NLRP3)*** conclusion,our results showed that tranylcypromine treatment ameliorated cochlear inflammation by promoting the expression of SESN2,which induced autophagy,thereby restricting NLRP3-related inflammasome signaling,alleviating cochlear hair cell loss,and protecting hearing *** findings suggest that inhibiting lysine-specific demethylase 1 is a

关键词： 4-hydroxynonenal apoptosis autophagy cleaved caspase-3 inflammation NOD-like receptor family pyrin domain-containing 3(NLRP3) noise-induced hearing loss oxidative stress Sestrin2 tranylcypromine

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Advances in genotypic antimicrobialresistance testing: a comprehensive review

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Science China Life Sciences 2025年第01期 130-143页

作者： Boheng Duan xianjun Zeng Junping Peng Huan Kui College of Nanchang University Department of Imaging The Second Affiliated Hospital of Nanchang University NHC key laboratory of Systems Biology of Pathogens National Institute of Pathogen BiologyChinese Academy of Medical Sciences & Peking Union Medical College key laboratory of Respiratory Disease Pathogenomics Chinese Academy of Medical Sciences & Peking Union Medical College key laboratory of Pathogen Infection prevention and Control (Ministry of Education) State Key Laboratory of Respiratory Health and MultimorbidityNational Institute of Pathogen BiologyChinese Academy of Medical Sciences & Peking Union Medical College

Antimicrobial resistance(AMR) represents a substantial threat to global public health, complicating the treatment of common infections and leading to prolonged illness and escalated healthcare expenses. To effectively combat AMR, timely and accurate detection is crucial for AMR surveillance and individual-based therapy. Phenotypic antibiotic resistance testing(AST) has long been considered the gold standard in clinical applications, serving as the foundation for clinical AMR diagnosis and optimized therapy. It has significantly contributed to ensuring patients′ health and the development of novel antimicrobials. Despite advancements in automated culture-based AST technologies,inherent limitations impede the widespread use of phenotypic AST in AMR surveillance. Genotypic AST technologies offer a promising alternative option, exhibiting advantages of rapidity, high sensitivity, and specificity. With the continuous advancement and expanding applications of genotypic AST technologies, such as microfluidics, mass spectrometry, and high-resolution melting curve analysis, new vigor has been injected into the development and clinical implementation of genotypic AST technologies. In this narrative review, we discuss the principles, applications, and advancements of emerging genotypic AST methods in clinical settings. The comprehensive review aims to highlight the significant scientific potential of emerging genotypic AST technologies in clinical AMR diagnosis, providing insights to enhance existing methods and explore novel approaches.

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Aptamer-functionalized nanoplatforms overcoming temozolomide resistance in synergistic chemo/photothermal therapy through alleviating tumor hypoxia

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Nano Research 2023年第7期16卷 9859-9872页

作者： Yun Zeng Linfei Zhao Ke Li Jingwen Ma Dan Chen Changhu Liu Wenhua Zhan Yonghua Zhan School of Life Science and Technology Xidian University and Engineering Research Center of Molecular and Neuro ImagingMinistry of EducationXi’an 710126China Department of Radiation Oncology General Hospital of Ningxia Medical UniversityYinchuan 750004China International Joint Research Center for Advanced Medical Imaging and Intelligent Diagnosis and treatment and xi’an key laboratory of Intelligent Sensing and Regulation of trans-Scale Life Information School of Life Science and TechnologyXidian UniversityXi’an 710126China xi’an key laboratory for prevention and treatment of common aging diseases Translational and Research Centre for Prevention and Therapy of Chronic DiseaseInstitute of Basic and Translational MedicineXi’an Medical UniversityXi’an 710021China Radiology Department CT and MRI RoomNinth Hospital of Xi’anXi’an 710054China

Tumor hypoxia is intimately associated with gliomas,which represents a significant threat to human health and are resistant to the first-line chemotherapeutic drug temozolomide(TMZ)due to *** this work,to overcome TMZ resistance in orthotopic gliomas,aptamer-functionalized liposomes are manufactured to encapsulate TMZ and photothermal agent IR780,and can cross the blood-brain barrier and actively target *** is possible to employ liposomes for both fluorescence and photoacoustic imaging simultaneously due to their stability and excellent photothermal conversion *** chemo/photothermal synergistic therapeutic effect of liposomes on gliomas is demonstrated by their abilities to target orthotopic gliomas,alleviate tumor hypoxia and consequently reverse resistance of glioma cells to TMZ,thereby extending the survival time of tumor-bearing mice,making the nanoplatforms and their synergistic chemo/photothermal therapy as a potential clinical treatment for gliomas.

关键词： aptamers blood-brain barrier gliomas liposomes photothermal therapy

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Bifunctional bridging capping layer enables 24.5% efficiency of perovskite solar cells with polymer-based hole transport materials

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Science China Chemistry 2025年第1期 350-359页

作者： Can Zhu Yiyang Wang Lei Meng Beibei Qiu Jing Li Shucheng Qin Ke Hu xin Jiang Wenbin Lai Minchao Liu Zhe Liu Chenxing Lu Jinyuan Zhang Yongfang Li Beijing National laboratory for Molecular Sciences CAS Key Laboratory of Organic Solids Institute of Chemistry Chinese Academy of Sciences School of Chemical Science University of Chinese Academy of Sciences key laboratory of Solid State Optoelectronic Devices of Zhejiang Province College of Physics and Electronic Information Engineering Zhejiang Normal University key laboratory of Photochemical Conversion and Optoelectronic Materials Technical Institute of Physics and ChemistryChinese Academy of Sciences laboratory of Advanced Optoelectronic Materials College of Chemistry Chemical Engineering and Materials ScienceSoochow University

Developing a bridge capping layer between perovskite and hole transport layer materials（HTMs） in the n-i-p perovskite solar cells（pero-SCs） is an effective approach to modify the morphology of HTMs and passivate the perovskite ***, we select the quinoxaline-based bifunctional passivation agent, quinoxalin-6-yl-methylamine hydrochloride（Qx MACl）,as the bridging layer, and a D-A copolymer PBQ12 containing the same quinoxaline unit as an HTM for the n-i-p pero-SCs. Due to the π-π stacking among the common quinoxaline units in the bridge layer and HTM, Qx MACl induces the π-π stacking of the PBQ12 film and improves the film morphology of HTMs with better conductivity. Additionally, Qx MACl can effectively passivate the perovskite surface, and PBQ12 possesses appropriate energy levels and high hole mobility. The pero-SCs based on FAPbI3with PBQ12/Qx MACl treatment showed a higher power conversion efficiency（PCE） of 24.05% and outstanding stability, maintaining 95.4% and 92.1% of its initial PCE after 750 h of storage and after over 800 h of thermal annealing at 85 °C, respectively. To further enhance the PCE of the PBQ12/Qx MACl-based devices, we developed a non-metal ion dopant for the PBQ12 HTM. Through trace doping of PBQ12 HTM by the non-metal ion dopant, the PCE of the PBQ12/Qx MACl-based devices reached 25.24%（the calibrated PCE of 24.55% by the National Institute of Metrology, China）.

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Prenatal cadmium exposure impairs neural tube closure via inducing excessive apoptosis in neuroepithelium

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Journal of Environmental Sciences 2024年第4期138卷 572-584页

作者： Shiyong Zhu Xuenan Li Xueyan Dai Jinlong Li College of Veterinary Medicine Northeast Agricultural UniversityHarbin 150030China key laboratory of the Provincial Education Department of Heilongjiang for common Animal Disease prevention and treatment Northeast Agricultural UniversityHarbin 150030China Heilongjiang key laboratory for laboratory Animals and Comparative Medicine Northeast Agricultural UniversityHarbin 150030China

Birth defects have become a public health *** hazardous environmental factors exposure to embryos could increase the risk of birth ***,a toxic environmental factor,can cross the placental barrier during *** woman may be subjected to cadmium before taking precautionary protective ***,the link between birth defects and cadmium remains *** exposure can induce excessive apoptosis in neuroepithelium during embryonic development *** exposure activated the p53 via enhancing the adenosine 5‘-monophosphate(AMP)-activated protein kinase(AMPK)and reactive oxygen species'(ROS)*** cadmium decreases the level of Paired box 3(Pax3)and murine double minute 2(Mdm2),disrupting the process of p53 *** p53 accumulation induced excessive apoptosis in neuroepithelium during embryonic development *** apoptosis led to the failure of neural tube *** study emphasizes that environmental materials may increase the health risk for *** caused the failure of neural tube closure during early embryotic *** women may be exposed by cadmium before taking precautionary protective actions,because of cadmium concentration-containing foods and environmental tobacco *** suggests that prenatal cadmium exposure is a threatening risk factor for birth defects.

关键词： Cadmium Neural tube closure Excessive apoptosis p53 Embryogenesis

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High frequency of thrombocytopenia in patients with acute-on-chronic liver failure treated with linezolid

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Hepatobiliary & Pancreatic diseases International 2015年第3期14卷 287-292页

作者： Yi-Min Zhang Wei Yu Ning Zhou Jian-Zhou Li Li-Chen Xu Zhong-Yang xie Ying-Feng Lu Lan-Juan Li State key laboratory for Diagnosis and treatment of Infectious diseases Department of Infectious Diseases the First Affiliated Hospital Zhejiang University School of Medicine Collaborative Innovation Center for Diagnosis and treatment of Infectious diseases

BACKGROUND： Linezolid is an effective antibiotic reagent for Gram-positive bacterial infection; its most common side effect is thrombocytopenia. However, the incidence of throm- bocytopenia in patients with acute-on-chronic liver failure （ACLF） who underwent linezolid therapy was unclear. The present study was to evaluate the incidence of thrombocyto- penia in ACLF and non-ACLF patients treated with linezolid and the risk factors of thrombocytopenia in these patients.

关键词： acute-on-chronic liver failure linezolid thrombocytopenia

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Effects of Salmonella infection on hepatic damage following acute liver injury in rats

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Hepatobiliary & Pancreatic diseases International 2016年第4期15卷 399-405页

作者： Yong-Tao Li Cheng-Bo Yu Dong Yan Jian-Rong Huang Lan-Juan Li State key laboratory for Diagnosis and treatment of Infectious diseases and Collaborative Innovation Center for Diagnosis and Treatment of Infectious DiseasesFirst Affiliated HospitalZhejiang University School of Medicine

BACKGROUND： Acute liver injury is a common clinical disorder associated with intestinal barrier injury and disturbance of intestinal microbiota. Probiotic supplementation has been reported to reduce liver injury; however, it is unclear whether enteropathogen infection exacerbates liver injury. The purpose of this study was to address this unanswered question using a rat model. METHODS： Oral supplementation with Salmonella enterica serovar enteritidis（S. enteritidis） was given to rats for 7 days. Different degrees of acute liver injury were then induced by intraperitoneal injection of D-galactosamine. The presence and extent of liver injury was assayed by measuring the concentrations of serum alanine aminotransferase, aspartate aminotransferase, and total bilirubin. Histology was used to observe liver tissue damage. Additionally, we measured the changes in plasma endotoxin, serum cytokines and bacterial translocation to clarify the mechanisms underlying intestinal microbiota associated liver ***： The levels of liver damage and endotoxin were significantly increased in the Salmonella infected rats with severe liver injury compared with the no infection rats with severe liver injury（P〈0.01）; The peyer＇s patch CD3＋ T cell counts were increased significantly when the Salmonella infection with severe injury group was compared with the normal group（P〈0.05）. S. enteritidis pretreatment enhanced intestinal barrier impairment and bacterial ***： Oral S. enteritidis administration exacerbates acute liver injury, especially when injury was *** factors of the exacerbation include inflammatory and oxidative stress injuries induced by the translocated bacteria and associated endotoxins, as well as over-activation of the immune system in the intestine and liver.

关键词： acute liver injury Salmonella enteritidis endotoxin cytokine bacterial translocation

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Prevalence and molecular characteristics of Listeria monocytogenes in cooked products and its comparison with isolates from listeriosis cases

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Frontiers of Medicine 2018年第1期12卷 104-112页

作者： Hong Wang Lijuan Luo Zhengdong Zhang Jianping Deng Yan Wang Yimao Miao Ling Zhang xi Chen xiang Liu Songsong Sun Bo xiao Qun Li Changyun Ye Zigong Center for Disease Control and prevention Zigong 643000 China State key laboratory of Infectious Disease prevention and Control National Institute for Communicable Disease Control and Prevention Collaborative Innovation Center for Diagnosis and Treatment of lnfectious Diseases Chinese Center for Disease Control and Prevention Beijing 102206 China

This study aimed to investigate the prevalence and molecular characteristics ofListeria monocytogenes in cooked products in Zigong City, China. The overall occurrence of the L. monocytogenes in the ready-to-eat （RTE） shops and mutton restaurants surveyed was 16.2% （141/873）. An occurrence of 13.5% was observed in RTE pork, 6.5% in RTE vegetables, and more than 24.0% in either cooked mutton or cooked haggis. Serotype 1/2b （45.4%）, 1/2a （33.3%）, and 1/2c （14.2%） were the predominant types. By comparing the clonal complexes （CCs） based on multilocus sequence typing （MLST） of the L. monocytogenes from cooked foods in Zigong City and 33 listeriosis cases from different districts of China, CC87, CC9, CC8, and CC3 were showed to be prevalent in cooked products and CC87 and CC3 were the first two frequent types in the 33 clinic-source strains. All CC87 stains harbored the newly reported Listeria pathogenicity island 4 （LIPI-4） gene fragment ptsA, and all CC3 strains possessed the Listeria pathogenicity island 3 （LIPI-3） gene fragment llsX. These may increase the occurrence of the strains belonging to CC87 and CC3 in listeriosis cases in China and also underline the risk of infection owing to the consumption of the cooked products from Zigong. ST619 （serotype 1/2b） harbored both llsX and ptsA, indicating a potential hypervirulent sequence type in Zigong.

关键词： Listeria monocytogenes MLST LIPI-3 LIPI-4 RTE listeriosis

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Bacteria Inactivation Using DBD Plasma Jet in Atmospheric Pressure Argon

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Plasma Science and Technology 2009年第1期11卷 83-88页

作者：许桂敏张冠军石兴民马跃王宁李元 State key laboratory of Electrical Insulation and Power Equipment School of Electrical EngineeringXi'an Jiaotong University Environment and Genes Related to diseases key laboratory of Education Ministry School of MedicineXi'an Jiaotong University

A coaxial dielectric barrier discharge plasma jet was designed, which can be operated in atmospheric pressure argon under an intermediate frequency sinusoidal resonant power supply, and an atmospheric pressure glow-like discharge was achieved. Two kinds of typical bacteria, i.e., the Staphylococcus aureus （S. aureus） and Escherichia coil （E. coil）, were employed to study the bacterial inactivation mechanism by means of the non-thermal plasma. The killing log value （KLV） of S. aureus reached up to 5.38 with a treatment time of 90 s and that of E. coil up to 5.36 with 60 s, respectively. According to the argon emission spectra of the plasma jet and the scanning electron microscope （SEM） images of the two bacteria before and after the plasma treatment, it is concluded that the reactive species in the argon plasma played a major role in the bacterial inactivation, while the heat, electric field and UV photons had little effect.

关键词： dielectric barrier discharge (DBD) atmospheric pressure glow-like discharge (APGLD) plasma jet non-thermal plasma inactivation bacteria

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