Prevalence and molecular characteristics of Listeria monocytogenes in cooked products and its comparison with isolates from listeriosis cases

作     者：Hong Wang Lijuan Luo Zhengdong Zhang Jianping Deng Yan Wang Yimao Miao Ling Zhang Xi Chen Xiang Liu Songsong Sun Bo Xiao Qun Li Changyun Ye 

作者机构：Zigong Center for Disease Control and Prevention Zigong 643000 China State Key Laboratory of Infectious Disease Prevention and Control National Institute for Communicable Disease Control and Prevention Collaborative Innovation Center for Diagnosis and Treatment of lnfectious Diseases Chinese Center for Disease Control and Prevention Beijing 102206 China 

出 版 物：《Frontiers of Medicine》 (医学前沿（英文版）)

年 卷 期：2018年第12卷第1期

页      面：104-112页

核心收录：

学科分类：082903[工学-林产化学加工工程] 07[理学] 08[工学] 0829[工学-林业工程] 070203[理学-原子与分子物理] 082201[工学-制浆造纸工程] 0822[工学-轻工技术与工程] 0702[理学-物理学] 

基　　金：supported by the Ministry of Science and Technology, China State Key Laboratory of Infectious Disease Prevention and Control, China CDC National Institute for Communicable Disease Control and Prevention, China CDC 

主　　题：Listeria monocytogenes MLST LIPI-3 LIPI-4 RTE listeriosis 

摘      要：This study aimed to investigate the prevalence and molecular characteristics ofListeria monocytogenes in cooked products in Zigong City, China. The overall occurrence of the L. monocytogenes in the ready-to-eat （RTE） shops and mutton restaurants surveyed was 16.2% （141/873）. An occurrence of 13.5% was observed in RTE pork, 6.5% in RTE vegetables, and more than 24.0% in either cooked mutton or cooked haggis. Serotype 1/2b （45.4%）, 1/2a （33.3%）, and 1/2c （14.2%） were the predominant types. By comparing the clonal complexes （CCs） based on multilocus sequence typing （MLST） of the L. monocytogenes from cooked foods in Zigong City and 33 listeriosis cases from different districts of China, CC87, CC9, CC8, and CC3 were showed to be prevalent in cooked products and CC87 and CC3 were the first two frequent types in the 33 clinic-source strains. All CC87 stains harbored the newly reported Listeria pathogenicity island 4 （LIPI-4） gene fragment ptsA, and all CC3 strains possessed the Listeria pathogenicity island 3 （LIPI-3） gene fragment llsX. These may increase the occurrence of the strains belonging to CC87 and CC3 in listeriosis cases in China and also underline the risk of infection owing to the consumption of the cooked products from Zigong. ST619 （serotype 1/2b） harbored both llsX and ptsA, indicating a potential hypervirulent sequence type in Zigong.