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Differences in leaf cuticular wax induced by whole-genome duplication in autotetraploid sour jujube

Horticultural plant Journal 2024年第1期10卷 66-76页

作者： Meng Li Chenxing Zhang Lu Hou Xinru Liu Hanqing Zhao Xiaoming Pang Wenhao Bo Yingyue Li National Engineering Research Center of tree breeding and Ecological Restoration College of Biological Sciences and TechnologyBeijing Forestry UniversityBeijing 100083China Key laboratory of Genetics and breeding in Forest trees and ornamental plants Ministry of EducationCollege of Biological Sciences and TechnologyBeijing Forestry UniversityBeijing 100083China The tree and ornamental plant breeding and biotechnology laboratory of National forestry and Grassland administration Beijing Forestry UniversityBeijing 100083China

Drought-resistant plants exhibit strong water retention *** this regard,the autotetraploid sour jujube leaves exhibit better water retention than diploid *** comparisons and physiological comparisons of diploid and autotetraploid leaves showed that the autotetraploid leaves had thicker leaf cuticles and more leaf wax accumulation than the diploid leaves,which could reduce cuticle permeability and improve the drought tolerance of *** this study,the cuticular wax crystalloids on the adaxial and abaxial sides of young and mature jujube leaves were observed in the two ploidy types,and unique cuticular wax crystalloids covering a large area of the cuticle on autotetraploid sour jujube leaves may provide an advantage in reducing leaf non-stomata transpiration and improving plant drought *** on the transcriptome,115 differentially expressed genes between diploids and autotetraploids were further analyzed and found to be involved in the accumulation of cuticular wax components,including terpenoids,fatty acids,and lipids,as well as ABC transporter and wax biosynthetic ***,14 genes differentially expressed between glossy autotetraploid leaves and nonglossy diploid leaves,such as LOC107414787,LOC107411574 and LOC107413721,were screened as candidate genes by qRT-PCR *** findings provided insights into how polyploidization improved drought tolerance.

关键词： Autotetraploid Jujube Drought tolerance Cuticular wax RNA-seq

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Computational tools for plant genomics and breeding

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Science China(Life Sciences) 2024年第8期67卷 1579-1590页

作者： Hai Wang Mengjiao Chen Xin Wei Rui Xia Dong Pei Xuehui Huang Bin Han state Key laboratory of Maize Bio-breeding Frontiers Science Center for Molecular Design BreedingJoint International Research Laboratory of Crop Molecular BreedingNational Maize Improvement CenterCollege of Agronomy and BiotechnologyChina Agricultural UniversityBeijing 100193China Sanya Institute of China Agricultural University Sanya 572025China Hainan Yazhou Bay Seed laboratory Sanya 572025China state Key laboratory of tree Genetics and breeding Key Laboratory of Tree Breeding and Cultivation of the State Forestry and Grassland AdministrationResearch Institute of ForestryChinese Academy of ForestryBeijing 100091China Shanghai Key laboratory of plant Molecular Sciences College of Life SciencesShanghai Normal UniversityShanghai 200234China College of Horticulture South China Agricultural UniversityGuangzhou 510640China National Center for Gene Research CAS Center for Excellence in Molecular Plant SciencesChinese Academy of SciencesShanghai 200233China

plant genomics and crop breeding are at the intersection of biotechnology and information *** by a combination of highthroughput sequencing,molecular biology and data science,great advances have been made in omics technologies at every step along the central dogma,especially in genome assembling,genome annotation,epigenomic profiling,and transcriptome *** advances further revolutionized three directions of *** is genetic dissection of complex traits in crops,along with genomic prediction and *** second is comparative genomics and evolution,which open up new opportunities to depict the evolutionary constraints of biological sequences for deleterious variant *** third direction is the development of deep learning approaches for the rational design of biological sequences,especially proteins,for synthetic *** three directions of development serve as the foundation for a new era of crop breeding where agronomic traits are enhanced by genome design.

关键词： bioinformatics plant genomics breeding by design

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Morphologic characters and element content during development of Pinus tabuliformis seeds

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Journal of forestry Research 2016年第1期27卷 67-74页

作者： Hui Li Jian Zhao Chen Zhou Scott A.Merkle Jin-Feng Zhang National Engineering laboratory for tree breeding College of Biological Sciences and Biotechnology Beijing Forestry University Key laboratory of Genetics and breeding in Forest trees and ornamental plants Ministry of Education Beijing Forestry University the tree and ornamental plant breeding and biotechnology laboratory of state forestry administration Beijing Forestry University Warnell School of forestry and Natural Resources The University of Georgia

An embryo classification system for Pinus tabuliformis Carr. was established by time-tracing sam- pling and observation of zygotic embryos. The zygotic embryos were divided into nine stages. Key elements of the zygotic embryo and female gametophyte （FG） tissue of P. tabuliformis were analyzed, using inductively coupled plasma-emission spectroscopy. Several elements--includ- ing aluminum, iron, sodium, and copper--are found in both embryo and FG tissue. Boron, phosphorus, magnesium,zinc, and calcium are also required for zygotic embryo development and therefore accumulated. Manganese is selectively excluded from the embryo. The zygotic embryo development needs a low-sodium and high-potassium nutrition proportion. The results of elemental analysis from zygotic embryos and FGs can provide the mineral targets for optimizing the formulation of culture medium for somatic embryogenesis.

关键词： Embryo classification system Zygoticembryo Female gametophyte Element content analysis

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Identification and Characterization of the Populus AREB/ABF Subfamily

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Journal of Integrative plant Biology 2013年第2期55卷 177-186页

作者： Lexiang Ji Jia Wang Meixia Ye Ying Li Bin Guo Zhong Chen Hao Li Xinmin An National Engineering laboratory for tree breeding NDRC Key laboratory of Genetics and breeding in Forest trees and ornamental plants MOE The tree and ornamental plant breeding and biotechnology laboratory SFA College of Biological Sciences and Biotechnology Beijing Forestry University

Abscisic acid （ABA） is a major plant hormone that plays an important role in responses to abiotic stresses. The ABA-responsive element binding protein/ABRE-binding factor （AREB/ABF） gene subfamily contains crucial transcription factors in the ABA-mediated signaling pathway. In this study, a total of 14 putative AREB/ABF members were identified in the Populus trichocarpa Torr. ＆ Gray. genome using five AREB/ABF amino acid sequences from Arabidopsis thaliana L. as probes. The 14 putative Populus subfamily members showed high protein similarities, especially in the basic leucine zipper （bZIP） domain region. A neighbor-joining analysis combined with gene structure data revealed homology among the 14 genes. The expression patterns of the Populus AREB/ABF subfamily suggested that the most abundant transcripts of 11 genes occurred in leaf tissues, while two genes were most transcribed in root tissues. Significantly, eight Populus AREB/ABF gene members were upregulated after treatment with 100 t^M exogenous ABA, while the other six members were downregulated. We identified the expression profiles of the subfamily members in Populus tissues and elucidated different response patterns of Populus AREB/ABF members to ABA stress. This study provided insight into the roles of Populus AREB/ABF homologues in plant response to abiotic stresses.

关键词： Arabidopsis thafiana Populus AREB/ABF bioinformatics qRT-PCR.

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Adaptive strategy of Nitraria sibirica to transient salt,alkali and osmotic stresses via the alteration of Na+/K+fluxes around root tips

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Journal of forestry Research 2023年第2期34卷 425-432页

作者： Xindi Mei Ting Dai Yingbai Shen Xinjiang Key laboratory of Biological Resources and Genetic Engineering College of Life Science and TechnologyXinjiang UniversityUrumqi 830046People’s Republic of China National Engineering laboratory for tree breeding Key Laboratory of Genetics and Breeding in Forest Trees and Ornamental PlantsMinistry of EducationThe Tree and Ornamental Plant Breeding and Biotechnology Laboratory of National Forestry and Grassland AdministrationCollege of Biological Sciences and BiotechnologyBeijing Forestry UniversityBeijing 100083People’s Republic of China

Nitraria sibirica *** an important shrub with a strong salt-alkali tolerance,but the mechanism underlying this tolerance remains *** this study,***,with salt-sensitive Vigna radiata(Linn.)Wilczek as the control,was subjected to transient salt stress(100 mM NaCl),alkali stress(50 mM Na_(2)CO_(3)),and osmotic stress(175 mM mannitol).The ionic fluxes of Na^(+)and K^(+)in the root apical region were *** show that,under salt and alkali stress,*** roots exhibited higher capacities to limit Na+influx and reduce K+efflux,thereby resulting in lower Na^(+)/K^(+)ratios compared with *** *** stress induced stronger Na^(+)influx and K+efflux in the root salt stress treatment;Na^(+)influx was mainly observed in the root cap,while K^(+)efflux was mainly observed in the elongation *** under osmotic stress,*** roots showed stronger Na+efflux and weaker K+efflux than *** ***+efflux was mainly observed in the root elongation zone,while K+efflux was in the root *** results reveal the ionic strategy of *** in response to transient salt,alkali,and osmotic stresses through the regulation of Na+/K+flux homeostasis.

关键词： Nirtaria sibirica Na^(+)/K^(+)fl uxes Na^(+)/K^(+)ratios Salt stress Alkali stress Osmotic stress

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Construction and analysis of a plant transformation binary vector pBDGG harboring a bi-directional promoter fusing dual visible reporter genes

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Journal of Genetics and Genomics 2008年第4期35卷 245-249页

作者： Chunxiao Zhang Ying Gai Wenqi Wang Yanyan Zhu Xuemei Chen Xiangning Jiang College of Bioscience and Bioengineering Hebei University of Science ＆ Technology Shijiazhuang 050018 China College of Life Sciences and biotechnology Beijing Forestry University The Tree and Ornamental Plant Breeding and Biotechnology Laboratory of State Forestry Administration Beijing 100083 China Department of Science and Technology Yanshan University Qinhuangdao 066004 China

The constitutive promoter of cauliflower mosaic virus 35S （CaMV 35S） is a polar unidirectional promoter and is widely used in plant genetic engineering. In the present study, the unidirectional CaMV 35S promoter has been modified to a bi-directional promoter by fusing its minimal promoter element to the 5＇ end of CaMV 35S promoter in the opposite orientation. To qualitatively and quantitatively estimate its bi-directional transcriptional function and activity, two visible reporter genes, gusA （13-glucuronidase, GUS） and gfp （green fluorescent protein, GFP）, were fused to the two ends of the promoter in bi-orientations ending with NOS terminator sequences, respectively. Stable expression of gusA and gfp genes in transgenic tobacco （Nicotiana tabacum L.） was visulized by histochemically staining for GUS and fluorescence microscopic observation under UV for GFP in transgenic plants. The expression of two reporter genes showed that the constructed bi-directional promoter did have the bi-directional transcriptional function in both expected orientations. The quantitative estimation of GUS and GFP were determined on a HITACHI F1000 Fluorescence Spectrophotometer with various wavelengths of excitation and emission. The GUS activity varied from g to 250 pmol 4-MU/min/mg protein and the GFP content varied from 0.9 to 1.8 μg/ mg protein in various lines of transgenic tobacco plants. Higher GUS activity generally coupled with lower GFP content, and vice versa.

关键词： bi-directional promoter gusA gene gfp gene Nicotiana tabacum L. expression

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Quantitative Analysis of Cytokinins in plants by High Performance Liquid Chromatography:Electronspray Ionization Ion Trap Mass Spectrometry

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Journal of Integrative plant Biology 2010年第10期52卷 925-932页

作者： Weiqi Chen Ying Gai Shichang Liu Renxiao Wang Xiangning Jiang College of Life Sciencesand biotechnology Bering Forestry University The Tree and Ornamental Plant Bree~ng and BiotechnologyLaboratory of Chinese Forestry Administration National Engineering Laboratory for Tree Breeding Beijing 100083 China Institute of Genetics and Developmental Biology Chinese Academy of Sciences Beijing 100101 China

The present paper introduces a highly sensitive and selective method for simultaneous quantification of 12 cytokinins （free form and their conjugates）. The method includes a protocol of extraction with methanol/water/formic acid （15/4/1, v/v/v） to the micro-scale samples, pre-purification with solid phase extraction （SPE） cartridges of the extracts, separation with a high performance liquid chromatography （HPLC） and detection by an electrospray ionization ion trap mass spectrometry （ESl-lon trap-MS） system in a consecutive ion monitoring （CRM） mode at the three stage fragmentation of mass spectrometry （MS3）. The lowest detection level of the cytokinins of the method reaches 0.1-2.0 pg with a very wide range of linear regression from 1-512 pg, at the coefficient factors of 0.98-0.99. The feasibility of this method has been proven in the application of the method to the analysis of the trace-amount contents of cytokinins in the micro-scale samples of various types of plant materials, such as aerial parts of rice and poplar leaves etc. 12 endogenous cytokinins had been identified and quantified in the plant tissues, with an acceptable relatively higher recovery rate from 40% to 70%.

关键词： Methanol Cytokinins Extraction Extracts Methods Mass spectrometry Weight Traps Trapping Ions

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Mating patterns and pollen dispersal in a Japanese larch(Larix kaempferi) clonal seed orchard： a case study

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Science China(Life Sciences) 2018年第9期61卷 1011-1023页

作者： Xingbin Chen Xiaomei Sun Leiming Dong Shougong Zhang state Key laboratory of tree Genetics and breeding Chinese Academy of ForestryBeijing100091China Key laboratory of tree breeding and Cultivation of state forestry administration Research Institute of Forestry Chinese Academy of ForestryBeijing 100091China Jiangxi Provincial Key Lab for plant biotechnology Jiangxi Academy of ForestryBeijing 100091 China

Pollination dynamics highly determines the genetic quality of seed orchard crops. However, there is less research about the effect of mating patterns on seed productivity of orchard crops. So far, clonal seed orchards have been producing genetically improved seedlings used for most Japanese larch(Larix kaempferi(Lamb.) Carr.) plantations in China. In the present study, a total of 17 highly variable simple sequence repeat(SSR) markers were used for genotyping a progeny trial population consisting of 647 open-pollinated progenies germinated from seeds which were collected from 63 maternal clones with 140 potential paternal clones in a Japanese larch clonal seed orchard in China. Paternity analysis was used in the present case study in order to evaluate the level of paternal gametic contribution, estimate pollen contamination and selfing rates, and investigate pollination patterns,pollen dispersal patterns and the impact of mating patterns on seed productivity of orchard crops. We observed 93.7% of the success rate of the parental assignment, unequal paternal gametic contribution(0–12.4%) with 6.3% of the progenies derived from pollen contamination or unsampled pollen donors, and absence of evidence for selfing. We also found that pollination rate highly depended on the distance between pollen donors and maternal parents, the majority of the identified crossing(65.7%)occurred between clones within a 150-m radius, and large variations in growth performance existed among the paternal halfsiblings. Progeny growth performance(diameter at breast(DBH) and height(HGT)) was measured at Age-20 in order to investigate the impact of mating patterns on timber production of orchard crops. As either the paternal or maternal, two clones(i.e., clones Z38 and Z62) were identified to have produced progenies with higher average stem volume breeding values than that of all of the progenies. Specifically, the genetic gains for volume were 3.53% for the two clones as paternal parents, and 8

关键词： Larix kaempferi simple sequence repeats(SSRs) paternity analysis pollen contamination pollen dispersal growth performance

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Genetic architecture of leaf morphology revealed by integrated trait module in Catalpa bungei

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Horticulture Research 2023年第4期10卷 161-173页

作者： Miaomiao Zhang Bingyang Liu Yue Fei Xiaowei Yang Linjiao Zhao Chaozhong Shi Yueying Zhang Nan Lu Chuangye Wu Wenjun Ma Junhui Wang state Key laboratory of tree Genetics and breeding Key Laboratory of Tree Breeding and Cultivation of State Forestry AdministrationResearch Institute of ForestryChinese Academy of ForestryBeijing 100091China Academy of Forest and Grassland Inventory and Planning National Forestry and Grassland AdministrationBeijing 100714China Wenxian forestry Science Research Institute Jiaozuo 454850China

Leaves are crucial for maintaining plant growth and development via photosynthesis,and their function is simultaneously regulated by a suite of phenotypic *** much is known about the genetic architecture of individual leaf traits,unraveling the genetic basis of complex leafmorphology remains a *** on the functional correlation and coordination ofmulti-traits,we divided 15 leaf morphological traits into three modules,comprising size(area,length,width,and perimeter),shape(leaf lobes,aspect ratio,circularity,rectangularity,and the relevant ratios),and color(red,green,and blue)for an ornamental tree species,Catalpa bungei.A total of 189 significant single-nucleotide polymorphisms were identified in the leaves of ***:35,82,and 76 in the size,shape,and color modules,*** quantitative trait loci were common between the size and shape modules,which were closely related according to phenotype correlation,genetic mapping,and mRNA *** color module was independent of *** changes in the aspect ratio,leaf lobe,and circularity suggest that these traits could be the core indicators of the leaf shape *** LAS and SRK genes,associated with leaf lobe and circularity,were found to function in plant defense mechanisms and the growth of *** associations between the SRK and CRK2 genes and the leaf lobe and circularity traits were further verified by *** findings demonstrate the importance of integrating multi-trait modules to characterize leaf morphology and facilitate a holistic understanding of the genetic architecture of intraspecific leaf morphology diversity.

关键词： circular integrating finding

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Exceptionally high genetic variance of the doubled haploid(DH)population of poplar

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林业研究:英文版 2023年第6期34卷 1941-1950页

作者： Caixia Liu Sui Wang Yi Liu Meng Wang Erqin Fan Chen Liu Shikai Zhang Chuanping Yang Junhui Wang Heike W.Sederoff Xiangling You Vincent L.Chiang Su Chen Ronald R.Sederoff Guanzheng Qu College of Life Science Northeast Forestry UniversityHarbin 150040People’s Republic of China state Key laboratory of tree Genetics and breeding Northeast Forestry UniversityHarbin 150040People’s Republic of China Key laboratory of Soybean Biology in Chinese Ministry of Education Northeast Agricultural UniversityHarbin 150030People’s Republic of China state Key laboratory of tree Genetics and breeding Key Laboratory of Tree Breeding and Cultivation of State Forestry AdministrationResearch Institute of ForestryChinese Academy of ForestryBeijing 100091People’s Republic of China Department of plant and Microbial Biology North Carolina State UniversityRaleighNC 27695USA Forest biotechnology Group Department of Forestry and Environmental ResourcesNorth Carolina State UniversityRaleighNC 27695USA

Doubled haploid(DH)plants have been widely used for breeding and biological research in *** ulus *** been used as model woody plant species for biological ***,the induction of DH poplar plants is onerous,and limited biological or breeding work has been carried out on DH individuals or *** this study,we provide an effective protocol for poplar haploid induction based on an anther culture method.A total of 96 whole DH plant lines were obtained using an F1hybrid of Populus simonii×*** as a donor *** phenotypes of the DH population showed exceptionally high variance when compared to those of half-sib progeny of the donor *** DH line displayed distinct features compared to those of the other DH lines or the donor ***,some excellent homozygous lines have the potential to be model plants in genetic and breeding studies.

关键词： Populus Anther culture Doubled haploid Genetic variance Flow cytometry

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