Quantitative Analysis of Cytokinins in Plants by High Performance Liquid Chromatography:Electronspray Ionization Ion Trap Mass Spectrometry

作     者：Weiqi Chen Ying Gai Shichang Liu Renxiao Wang Xiangning Jiang 

作者机构：College of Life Sciencesand Biotechnology Bering Forestry University The Tree and Ornamental Plant Bree~ng and BiotechnologyLaboratory of Chinese Forestry Administration National Engineering Laboratory for Tree Breeding Beijing 100083 China Institute of Genetics and Developmental Biology Chinese Academy of Sciences Beijing 100101 China 

出 版 物：《Journal of Integrative Plant Biology》 (植物学报（英文版）)

年 卷 期：2010年第52卷第10期

页      面：925-932页

核心收录：

学科分类：0710[理学-生物学] 071010[理学-生物化学与分子生物学] 081704[工学-应用化学] 07[理学] 08[工学] 0817[工学-化学工程与技术] 09[农学] 0902[农学-园艺学] 090201[农学-果树学] 

基　　金：supported by the National Natural Science Foundation of China (30630053) to Dr Xiangning Jiang and well-advised and directed by Dr Jiayang Li 

主　　题：Methanol Cytokinins Extraction Extracts Methods Mass spectrometry Weight Traps Trapping Ions 

摘      要：The present paper introduces a highly sensitive and selective method for simultaneous quantification of 12 cytokinins （free form and their conjugates）. The method includes a protocol of extraction with methanol/water/formic acid （15/4/1, v/v/v） to the micro-scale samples, pre-purification with solid phase extraction （SPE） cartridges of the extracts, separation with a high performance liquid chromatography （HPLC） and detection by an electrospray ionization ion trap mass spectrometry （ESl-lon trap-MS） system in a consecutive ion monitoring （CRM） mode at the three stage fragmentation of mass spectrometry （MS3）. The lowest detection level of the cytokinins of the method reaches 0.1-2.0 pg with a very wide range of linear regression from 1-512 pg, at the coefficient factors of 0.98-0.99. The feasibility of this method has been proven in the application of the method to the analysis of the trace-amount contents of cytokinins in the micro-scale samples of various types of plant materials, such as aerial parts of rice and poplar leaves etc. 12 endogenous cytokinins had been identified and quantified in the plant tissues, with an acceptable relatively higher recovery rate from 40% to 70%.