Genetic architecture of leaf morphology revealed by integrated trait module in Catalpa bungei

作     者：Miaomiao Zhang Bingyang Liu Yue Fei Xiaowei Yang Linjiao Zhao Chaozhong Shi Yueying Zhang Nan Lu Chuangye Wu Wenjun Ma Junhui Wang Miaomiao Zhang;Bingyang Liu;Yue Fei;Xiaowei Yang;Linjiao Zhao;Chaozhong Shi;Yueying Zhang;Nan Lu;Chuangye Wu;Wenjun Ma;Junhui Wang

作者机构：State Key Laboratory of Tree Genetics and BreedingKey Laboratory of Tree Breeding and Cultivation of State Forestry AdministrationResearch Institute of ForestryChinese Academy of ForestryBeijing 100091China Academy of Forest and Grassland Inventory and PlanningNational Forestry and Grassland AdministrationBeijing 100714China Wenxian Forestry Science Research InstituteJiaozuo 454850China 

出 版 物：《Horticulture Research》 (园艺研究（英文）)

年 卷 期：2023年第10卷第4期

页      面：161-173页

核心收录：

学科分类：0710[理学-生物学] 090706[农学-园林植物与观赏园艺] 0907[农学-林学] 09[农学] 0902[农学-园艺学] 

基　　金：supported by the National Key Research and Development Plan of China(2021YFD2200202) the National Natural Science Foundation of China(32001337)。 

主　　题：circular integrating finding 

摘      要：Leaves are crucial for maintaining plant growth and development via photosynthesis,and their function is simultaneously regulated by a suite of phenotypic traits.Although much is known about the genetic architecture of individual leaf traits,unraveling the genetic basis of complex leafmorphology remains a challenge.Based on the functional correlation and coordination ofmulti-traits,we divided 15 leaf morphological traits into three modules,comprising size(area,length,width,and perimeter),shape(leaf lobes,aspect ratio,circularity,rectangularity,and the relevant ratios),and color(red,green,and blue)for an ornamental tree species,Catalpa bungei.A total of 189 significant single-nucleotide polymorphisms were identified in the leaves of C.bungei:35,82,and 76 in the size,shape,and color modules,respectively.Four quantitative trait loci were common between the size and shape modules,which were closely related according to phenotype correlation,genetic mapping,and mRNA analysis.The color module was independent of them.Synergistic changes in the aspect ratio,leaf lobe,and circularity suggest that these traits could be the core indicators of the leaf shape module.The LAS and SRK genes,associated with leaf lobe and circularity,were found to function in plant defense mechanisms and the growth of leaves.The associations between the SRK and CRK2 genes and the leaf lobe and circularity traits were further verified by RT-qPCR.Our findings demonstrate the importance of integrating multi-trait modules to characterize leaf morphology and facilitate a holistic understanding of the genetic architecture of intraspecific leaf morphology diversity.