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Dynamics and roles of phragmoplast microfilaments in cell plate formation during cytokinesis of tobacco BY-2 cells

Chinese Science Bulletin 2009年第12期54卷 2051-2061页

作者： ZHANG Yan ZHANG WenJie BALUSKA Frantisek MENZEL Diedrik REN HaiYuni key laboratory of cell proliferation and regulation biology of ministry of education Beijing Normal University Beijing 100875 China Institute of cellular and Molecular Botany University of Bonn D-53115 Bonn Germany

The phragmoplast is a special apparatus that functions in establishing a cell plate in dividing plant cells. It is known that microfilaments (MFs) are involved in constituting phragmoplast structure, but the dynamic distribution and role of phragmoplast MFs are far from being understood. In this study, the precise structure and dynamics of MFs during the initiation and the late lateral expansion of the phragmoplast were observed by using a tobacco BY-2 cell line stably expressing the microfilament reporter construct GFP-fABD2. Three-dimensional imaging showed that the phragmoplast MFs were initiated by two populations of MFs emerging between the reconstituting daughter nuclei at anaphase, which migrated to the mid-zone and gave rise to two layers of microfilament arrays. FM4-64 stained vesicles accumulated and fused with the cell plate between the two populations of MFs. The two layers of microfilament arrays of phragmoplast with ends overlapped always surrounded the centrifugally expanding cell plate. Partial disruption of MFs at metaphase by low concentration of latrunculin B resulted in the inhibition of the cell plate consolidation and the blockage of cell plate lateral expansion, whereas high concentration of latrunculin B restrained the progression of the cell cycle. Treating the cell after the initiation of phragmoplast led to the cease of the expansion of the cell plate. Our observations provide new insights into the precise structure and dynamics of phragmoplast MFs during cytokinesis and suggest that dynamic phragmoplast MFs are important in cell plate formation.

关键词：植物细胞板形微丝烟草胞质成膜结构绿色荧光蛋白 MFs

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Targeting histones for degradation in cancer cells as a novel strategy in cancer treatment

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Science China(Life Sciences) 2019年第8期62卷 1078-1086页

作者： Yesheng Yin Qianqian Zhu Tianxia Jiang Libin Fan Xiaobo Qiu College of Life Sciences Anhui Medical UniversityHefei230032China key laboratory of cell proliferation and regulation biology Ministry of Educationand College of Life SciencesBeijing Normal UniversityBeijing100875China

The anticancer therapies with the joint treatment of a histone deacetylase(HDAC) inhibitor and a DNA-damaging approach are actively under clinical investigations, but the underlying mechanism is unclear. Histone homeostasis is critical to genome stability, transcriptional accuracy, DNA repair process, senescence, and survival. We have previously demonstrated that the HDAC inhibitor, trichostatin A(TSA), could promote the degradation of the core histones induced by γ-radiation or the DNAalkylating agent methyl methanesulfonate(MMS) in non-cancer cells, including mouse spermatocyte and embryonic fibroblast cell lines. In this study, we found that the joint treatment by TSA and MMS induced the death of the cultured cancer cells with an additive effect, but induced degradation of the core histones synergistically in these cells. We then analyzed various combinations of other HDAC inhibitors, including suberoylanilide hydroxamic acid and valproate sodium, with MMS or other DNAdamaging agents, including etoposide and camptothecin. Most of these combined treatments induced cell death additively, but all the tested combinations induced degradation of the core histones synergistically. Meanwhile, we showed that cell cycle arrest might not be a primary consequence for the joint treatment of TSA and MMS. Given that clinic treatments of cancers jointly with an HDAC inhibitor and a DNA-damaging approach often show synergistic effects, histone degradation might more accurately underlie the synergistic effects of these joint treatments in clinic applications than other parameters, such as cell death and cell cycle arrest. Thus, our studies might suggest that the degradation of the core histones can serve as a new target for the development of cancer therapies.

关键词： HDAC histone deacetylase inhibitor DNA damage anticancer agent histone degradation

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Crucial role of histone deacetylase SIRT1 in myeloid-derived suppressor cell-mediated reprogramming of CD4^(+) T-cell differentiation

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cellular & Molecular Immunology 2020年第7期17卷 785-787页

作者： Lin Dong Yujing Bi Anna Jia Qing Yu Yuexin Wang Yufei Wang Qiuli Yang Yejin Cao Ying He Ruichen Liu Yan Li Guangwei Liu key laboratory of cell proliferation and regulation biology Ministry of EducationInstitute of Cell BiologyCollege of Life SciencesBeijing Normal University100875 BeijingChina State key laboratory of Pathogen and Biosecurity Beijing Institute of Microbiology and Epidemiology100071 BeijingChina

Myeloid-derived suppressor cells(MDSCs)are heterogeneous,immature myeloid cells that inhibit the immune responses of T cells.1–5 MDSCs play a crucial role in infection,6,7 transplantation,8,9 autoimmune diseases10,11,and tumors12,13 by driving the differentiation of specific T-cell subsets,but the precise regulatory mechanism is still ***,our results showed that SIRT1,a histone deacetylase,suppresses the functions of proinflammatory MDSCs and promotes tumor growth.14 However,it remains unclear whether SIRT1 regulates the MDSC-induced differentiation of T ***,we identified the regulatory effects of SIRT1 in CD11b+Gr1+MDSCs on T-cell differentiation.

关键词： SIRT1 CD11b myeloid

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Role of Ppt1 in multiple stress responses in Candida albicans

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Chinese Science Bulletin 2014年第31期59卷 4060-4068页

作者： Kangdi Hu Wanjie Li Jiaxin Gao Qizheng Liu Haitao Wang Yue Wang Jianli Sang key laboratory of cell proliferation and regulation biology Ministry of EducationCollege of Life ScienceBeijing Normal University School of Biotechnology and Food Engineering Hefei University of Technology Institute of Molecular and cell biology Agency for ScienceTechnology and Research (A*STAR)

To study the function of CaPpt1,we deleted PPT1 gene from the Candida albicans genome by sequentially replacing the entire coding region with the selectable markers ARG4 and *** results showed that the deletion of Ppt1 did not affect the hyphal formation of *** under serum induction and caused enhanced sensitivity to DNA damage,Calcofluor white and saltinduced *** also found that Ppt1 was not required for the phenotypic response of cells treated with the genotoxins,methylmethane sulfonate and *** cytometric analyses indicated that ppt1D cells and wild-type cells showed similar G2/M arrest profiles when exposed to DNA damage ***1 was not required for the activation of the DNA damage response pathway,as indicated by normal phosphorylation of Rad53 and Rfa2 in ppt1D cells under DNA damage *** suggest that Ppt1 plays important roles in response to various stress conditions in ***.

关键词：白色念珠菌应激反应流式细胞分析 DNA损伤选择性标记基因组胁迫条件编码区

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Sterility and fertility:keys for crop production in China

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Science China(Life Sciences) 2010年第12期53卷 1488-1489页

作者： Sodmergen key laboratory for cell proliferation and Differentiation(ministry of education of China) College of Life SciencesPeking University

Agricultural production is among the most important issues in China, since the country possesses a population exceeding 1.3 billion people. The arable land per capita, however, comprises only about 1/3 of the world av... 详细信息

关键词： Sterility and fertility

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Arabidopsis RAN 1 Mediates Seed Development through Its Parental .Ratio by Affecting the Onset of Endosperm cellularization

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Molecular Plant 2014年第8期7卷 1316-1328页

作者： Peiwei Liu Ming Qi Yuqian Wang MingQin Chang Chang Liu Mengxiang Sun Weicai Yang Haiyun Ren key laboratory of cell proliferation and regulation biology of ministry of education College of Life Sciences Beijing NormalUniversity Beijing 100875 China Department of cell and Development biology College of Life Sciences State Key Laboratory of Hybrid Rice Wuhan University Wuhan430072 China laboratory of Molecular and Developmental biology Institute of Genetics and Developmental Biology Chinese Academy of SciencesBeijing 100101 China

Although previous studies have demonstrated that endosperm development is influenced by its parental genome constitution, the genetic basis and molecular mechanisms that control parent-of-origin effects require further elucidation. Here we show that the Ras-related nuclear protein 1 （RAN1） regulates endosperm development in Arabidopsis thaliana. Reciprocal crosses between wild-type （WT） and transgenic lines misexpressing RAN1 （msRAN1） gave rise to small F1 seeds when RAN1 down-regulated/up-regulated individuals were used as a male/female parent; in contrast, F1 seeds were aborted when RAN1 down-regulated/up-regulated plants were used as a female/male parent, suggesting that seed development is affected by the parental genome ratio of RAN1. Whereas RAN1 expression in wild-type plants is reduced before the onset of endosperm cellularization, F1 seeds from reciprocal crosses between WT and msRAN1 showed abnormal endosperm cellularization and ectopic expression of RAN1. The expression of MINISEED3 （MINI3）-a gene that also controls endosperm cellularization-was also affected in these reciprocal crosses, and the misregulation of MINI3 activity rescued F1 seeds when msRAN1 plants were used in reciprocal crosses. Taken together, our results suggest that the parental ratio of RAN1 regulates the onset of endosperm cellularization through its genetic interaction with MINI3.

关键词： RAN1 seed development parental ratio endosperm cellularization.

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Discovery and identification of anti-U1-A snRNP antibody in lung cancer

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Science China(Life Sciences) 2005年第6期48卷 641-647页

作者： ZHANG Lijuan1, LIU Jifu2, ZHANG Hao1, WU Shanshan2, HUANG Lingyun1, HE Dacheng1 & XIAO Xueyuan1 1. key laboratory of cell proliferation and regulation of ministry of education, Beijing Normal University, Beijing 100875, China 2. Department of Thoracic Surgery, General Hospital of Beijing Unit, PLA, Beijing 100700, China key laboratory of cell proliferation and regulation of ministry of education Beijing Normal University Beijing China Department of Thoracic Surgery General Hospital of Beijing Unit PLA Beijing China

There are multiple reports of autoimmune response in patients with lung cancer. To investigate whether a novel autoantibody is present in patients with lung cancer and evaluate its clinical diagnostic and prognostic value, sera from 10 patients with lung cancer and 10 normal individuals were analyzed using immunofluorescence and Western blotting. It was found that one serum sample from the patients with squamous carcinoma gave a fine speckled pattern staining in nucleus and had a high titer antinuclear autoantibody which could recognize 31 kD of nuclear protein isolated from both cancer cells and normal cells. The same patient’s serum was further used to immunoprecipitate the target antigen. The protein bands were excised from the SDS-PAGE gels and were analyzed with a Qstar Pulser I Quadrupole time-flight mass spec-trometer, and the 31 kD target antigen was identified as U1-A snRNP. To test the prevalence of anti-U1-A snRNP antibody, sera from 93 patients including 36 squmaous carcinomas (SCC), 26 adenocarcinomas (Ad), and 31 small cell carcinomas (SCLC) were screened by Western blotting. The results demonstrated that anti-U1-A snRNP antibody was present in 50% of SCC sera, 26.9% of Ad sera and 54.8% of SCLC sera. In this paper, we report for the first time that anti-U1-A snRNP antibody could be detected in the patients with lung cancer.

关键词： lung cancer, autoantibody, U1-A snRNP.

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C-terminal domain of Chk1 regulates its subcellular location and kinase activity for DNA repair

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Chinese Science Bulletin 2011年第30期56卷 3138-3147页

作者： NING LiFeng WANG HuiPing SAN Dan SANG JianLi key laboratory for cell proliferation and regulation biology of ministry of education College of Life Science Beijing Normal University Beijing 100875 China Department of Reproduction Immunology National Research Institute for Family Planning Beifing 100081 China

Effector kinase Chk1 is an evolutionarily conserved protein kinase. It is a key mediator linking the mechanisms that monitor DNA integrity to components of the cell cycle engine. In this study, recombinant vectors pEGFP-C1-Chk1/C 288/C 334/C 368 were constructed and transfected into HeLa cells to study the effect of the Chk1 regulatory domain on the regulation of subcellular Chk1 location in response to DNA damage. We found that DNA damage-induced nuclear accumulation is regulated by 34 amino acids (334–368) in the C-terminal regulatory domain. Recombinant vectors pXJ41-Chk1/C 288/C 334/C 368 were co-transfected with reporter plasmid pEGFP-N2 into HeLa cells to study the repair abilities of the different human Chk1 truncation mutants. In addition, recombinant vectors were transfected into HeLa cells to study the effects of the different truncation mutants on the cell cycle. Furthermore, to study the kinase activity of the different truncation mutants, Ser216 phosphorylation of Cdc25C was studied by Western blot analysis. We found that the enzymatic activity of C 368, missing the 108 C-terminal amino acids (368–476), was higher than that of full-length Chk1, and C 368 delayed the cell cycle progression. The enzymatic activity of C 334, missing the 142 C-terminal amino acids (334–476), was equivalent to that of full-length Chk1. C 288, missing the 188 C-terminal amino acids (288–476), had almost no enzymatic activity, suggesting that the regulatory domain contains both inhibitory and regulatory elements. This study provides useful information for further research on Chk1 function.

关键词：亚细胞定位激酶活性 DNA修复 HeLa细胞 DNA损伤细胞周期重组载体 blot分析

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In vitro assembly of plant tubulin in the absence of microtubule-stabilizing reagents

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Chinese Science Bulletin 2000年第24期45卷 2258-2263页

作者： HUANG Shanjin REN Haiyun YUAN Ming key laboratory of Plant Physiology and Biochemistry of ministry of Agriculture Department of Plant SciencesCollege of Biological SciencesChina Agricultural UniversityBeijing 100094China key laboratory of cell proliferation and regulation biology of ministry of education College of Life SciencesBeijing Normal UniversityBeijing 100875China

The assembly of microtubules is essential for physiological functions of microtubules. Addition of microtubule-stabilizing reagents or microtubule 'seeds' is usually necessary for plant tubulin assembly in vitro, which hinders the investigation of plant microtubule dynamics. In the present note, highly purified plant tubulins have been obtained from lily pollen, a non-microtubule-stabilizing reagent or microtubule 'seed' system for plant tubulin assembly has been established and the analysis of plant tubulin assembly performed. Experiment results showed that purified tubulin polymerized in vitro, and a typical microtubule structure was observed with electron microscopy. The kinetics curve of tubulin assembly exhibited typical 'parabola'. The presence of taxol significantly altered the character of plant tubulin assembly, including that abnormal microtubules were assembled and the critical concentration for plant tubulin assembly was decreased exceedingly from 3 mg/mL in the absence of taxol to 0.043 mg/

关键词： lily pollen tubulin microtubule tubulin assembly.

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A Processive Arabidopsis Formin Modulates Actin Filament Dynamics in Association with Profilin

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Molecular Plant 2016年第6期9卷 900-910页

作者： Sha Zhang Chang Liu Jiaojiao Wang Zhanhong Ren Christopher J. Staiger Haiyun Ren key laboratory of cell proliferation and regulation biology of ministry of education College of Life Science Beijing Normal University Beijing 100875 People'sRepublic of China Department of Biological Sciences Purdue University Hansen Life Sciences Research Building West Lafayette IN 47907-2064 USA

Formins are conserved regulators of actin cytoskeletal organization and dynamics that have been impli- cated to be important for cell division and cell polarity. The mechanism by which diverse formins regulate actin dynamics in plants is still not well understood. Using in vitro single-molecule imaging technology, we directly observed that the FH1-FH2 domain of an Arabidopsis thaliana formin, AtFH14, processively at- taches to the barbed end of actin filaments as a dimer and slows their elongation rate by 90%. The attach- ment persistence of FH1-FH2 is concentration dependent. Furthermore, by use of the triple-color total internal reflection fluorescence microscopy, we found that ABP29, a barbed-end capping protein, com- petes with FH1-FH2 at the filament barbed end, where its binding is mutually exclusive with AtFH14. In the presence of different plant profilin isoforms, FH1-FH2 enhances filament elongation rates from about 10 to 42 times. Filaments buckle when FH1-FH2 is anchored specifically to cover slides, further indicating that AtFH 14 moves processively on the elongating barbed end. At high concentration, AtFH 14 bundles actin filaments randomly into antiparallel or parallel spindle-like structures; however, the FH1-FH2-mediated bundles become thinner and longer in the presence of plant profilins. This is the direct demonstration of a processive formin from plants. Our results also illuminate the molecular mechanism of AtFH14 in regulating actin dynamics via association with profilin.

关键词： processive formin profilin capping protein actin bundle TIRF microscopy single molecule

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