In vitro assembly of plant tubulin in the absence of microtubule-stabilizing reagents

作     者：HUANG Shanjin REN Haiyun YUAN Ming 

作者机构：Key Laboratory of Plant Physiology and Biochemistry of Ministry of AgricultureDepartment of Plant SciencesCollege of Biological SciencesChina Agricultural UniversityBeijing 100094China Key Laboratory of Cell Proliferation and Regulation Biology of Ministry of EducationCollege of Life SciencesBeijing Normal UniversityBeijing 100875China 

出 版 物：《Chinese Science Bulletin》 (中国科学通报)

年 卷 期：2000年第45卷第24期

页      面：2258-2263页

核心收录：

学科分类：0710[理学-生物学] 071001[理学-植物学] 07[理学] 

基　　金：National Key Basic Research Project, (G19990117) National Natural Science Foundation of China, NSFC, (19892380,39670355, 25003, 39870052) National Natural Science Foundation of China, NSFC 

主　　题：lily pollen tubulin microtubule tubulin assembly. 

摘      要：The assembly of microtubules is essential for physiological functions of microtubules. Addition of microtubule-stabilizing reagents or microtubule seeds is usually necessary for plant tubulin assembly in vitro, which hinders the investigation of plant microtubule dynamics. In the present note, highly purified plant tubulins have been obtained from lily pollen, a non-microtubule-stabilizing reagent or microtubule seed system for plant tubulin assembly has been established and the analysis of plant tubulin assembly performed. Experiment results showed that purified tubulin polymerized in vitro, and a typical microtubule structure was observed with electron microscopy. The kinetics curve of tubulin assembly exhibited typical parabola . The presence of taxol significantly altered the character of plant tubulin assembly, including that abnormal microtubules were assembled and the critical concentration for plant tubulin assembly was decreased exceedingly from 3 mg/mL in the absence of taxol to 0.043 mg/