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Candidate reference measurement procedure for serum 17-hydroxyprogesterone quantification via isotope dilution liquid chromatography–tandem mass spectrometry

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iLABMED 2024年第2期2卷 98-107页

作者： Ji Zhang Yang Jiang Ting Liu Xiongwei Liu Hui Huang Yanxiang Cheng Jieyan Li Bangning Cheng Chungen Qian Xufu Xiang Jun-Fa Xu Guangdong Provincial key laboratory of Medical molecular Diagnostics Department of Clinical ImmunologyInstitute of Laboratory MedicineSchool of Medical TechnologyGuangdong Medical UniversityDongguanGuangdongChina Shenzhen YHLO Biotech Co. Ltd.ShenzhenGuangdongChina Shenzhen Longgang Central Hospital Clinical laboratory ShenzhenGuangdongChina The key laboratory for biomedical photonics of moe at wuhan national laboratory for optoelectronics-hubei bioinformatics and molecular imaging key laboratory Systems Biology ThemeDepartment of Biomedical EngineeringCollege of Life Science and TechnologyHuazhong University of Science and TechnologyWuhanHubeiChina

Background:Accurate quantification of 17-hydroxyprogesterone(17-OHP)in serum is vital for clinical and research ***,inter-laboratory variability in test results exists owing to the lack of a standardized reference measurement procedure(RMP).Therefore,in this study,we developed a highly accurate,cost-effective,and user-friendly candidate RMP(cRMP)for analyzing 17-OHP in ***:We quantified 17-OHP in serum using a one-step liquid–liquid extraction method with the addition of 17-OHP-^(13)C_(3),followed by liquid chromatography–tandem mass *** ability of these methods to suppress interference was evaluated by chromatographic *** assessed accuracy,specificity,the lower limit of quantitation,linearity,and matrix effects by following the standards specified by the Clinical and laboratory Standards *** consistency between the developed cRMP and the chemiluminescence method was evaluated through experiments with 120 clinical ***:The developed cRMP required only 8 min for accurate quantification of serum 17-OHP without bias from matrix effects or interference from 19 metabolites added as potential *** method exhibited favorable measurement performance,with a quantitation limit of 0.086 ng/mL,linear range of 0.1–400 ng/mL,a total imprecision of≤2.90%,spike recovery of 100.1%–100.6%,and relative deviations from assigned target values(RfB Institution)of−2.91%to 1.10%.The cRMP demonstrated good consistency with the conventional assay(chemiluminescence method),with a correlation coefficient R of ***:A cRMP with high accuracy,cost-effectiveness,and convenient operation was developed for quantifying 17-OHP in *** simplicity and robust performance make it an invaluable addition to the arsenal of analytical tools available for *** method can enhance the accuracy and reliability of 17-OHP measurements across various laboratories.

关键词： 17-hydroxyprogesterone isotope dilution liquid chromatography-tandem mass spectrometry reference measurement procedure serum

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生物信息学资源助力蛋白质磷酸化的系统层次理解

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Science Bulletin 2024年第8期69卷 989-992页

作者：彭迪贾大夏宏光张珞颖黄鹏宇薛宇 Department of bioinformatics and Systems Biology MOE Key Laboratory of Molecular BiophysicsHubei Bioinformatics and Molecular Imaging Key LaboratoryCollege of Life Science and TechnologyHuazhong University of Science and TechnologyWuhan 430074China key laboratory of Birth Defects and Related Diseases of Women and Children Department of PaediatricsWest China Second University HospitalState Key Laboratory of BiotherapySichuan UniversityChengdu 610041China Department of Biochemistry&Research Center of Clinical Pharmacy of The First Affiliated Hospital Zhejiang University School of MedicineHangzhou 310058China Institute of biomedical Engineering Chinese Academy of Medical Sciences and Peking Union Medical CollegeTianjin 300192China Nanjing University Institute of Artificial Intelligence Biomedicine Nanjing 210031China

Protein phosphorylation,catalyzed by protein kinases(PKs),is one of the most prevalent post-translational modifications(PTMs)in *** occurring at different positions in a protein sequence can possess distinct functional impacts,and sitespecific phosphorylation may drastically alter a protein’s conformation,activity,and ***,biologists usually focused on studying regulatory mechanisms of phosphorylation in a limited number of proteins,mainly due to a lack of highthroughput *** the past two decades,advances in liquid chromatography coupled with tandem mass spectrometry(LC-MS/MC)have boomed a revolutionary technology named phosphoproteomics,which can simultaneously quantify thousands of phosphorylation sites(p-sites),and provide a great opportunity for a systems-level understanding of *** LCMS/MC,other approaches such as immunohistochemistry(IHC)and immune-detection by sequencing(ID-seq)have also emerged.

关键词： positions catalyzed translational

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Modulation of tumor microenvironment by metal-organic-framework-derived nanoenzyme for enhancing nucleus-targeted photodynamic therapy

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Nano Research 2020年第6期13卷 1527-1535页

作者： Xuemei Zeng Shuangqian Yan Peng Chen Wei Du Bi-Feng Liu The key laboratory for biomedical photonics of moe at wuhan national laboratory for optoelectronics-hubei bioinformatics&molecular imaging key laboratory Systems Biology ThemeDepartment of Biomedical EngineeringCollege of Life Science and TechnologyHuazhong University of Science and TechnologyWuhan430074China

Photodynamic therapy(PDT)is a promising strategy for tumor ***,its therapeutic efficacy is compromised by the unsatisfactory cytotoxicity to specific subcellular organelles and insidious tumor microenvironment properties like hypoxia and high glutathione ***,we fabricated a novel nanoenzyme that derived from metal-organic framework(MOF)with intrinsic catalase-like activities to decompose H2O2 to O2 and simultaneous glutathione consumption for enhancing PDT *** obtained Mn3O4 nanoparticle shows a larger pore size and surface area compared to native MOF particles,which can be used to load high dose *** decorated with AS1411 aptamer and polyethylene glycol(PEG),the obtained Mn3O4-PEG@C&A particle exhibits excellent stability and cell nucleus targeting ***,Mn3O4-PEG@C&A particle inhibited the tumor growth in the mouse model with high efficacy without any *** is the first report that applied MOF-derived nanoparticle to nucleus-targeted *** may provide a new approach for designing functional nanoenzyme to subcellular organelles-targeted tumor modulation.

关键词： nanoenzyme metal-organic framework catalase-like activity GSH consumption photodynamic therapy

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Characterization and Crystal Structure of a New Holmium(III) Complex with the Macrocyclic Ligand Derived from 2,6-Diformyl-4-methylphenol and Diethylenetriamine

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Chinese Journal of Structural Chemistry 2007年第12期26卷 1429-1434页

作者：胡学雷邱立赵元弟key laboratory of biomedical photonics of Ministry of Education - wuhan national laboratory for optoelectronics - hubei bioinformatics and molecular imaging key laboratory - key laboratory of molecular Biophysics of the Ministry of Education Huazhong University of Science and Technology 潘志权 key laboratory of biomedical photonics of Ministry of Education - wuhan national laboratory for optoelectronics - hubei bioinformatics and molecular imaging key laboratory - key laboratory of molecular Biophysics of the Ministry of Education Huazhong University of Science and Technology hubei key laboratory of Novel Chemical Reactor and Green Chemical Technology Wuhan Institute of Technology

The title compound （13,27-dimethyl-3,6,9,17,20,23-hexaazatricyclo-[23.***.1.1^11.15]- triaconta-l（29）,2,9,11,13,15（30）,16,23,25,27-decaene-29,30-diol-N^3,N^6,N^9,O^29,O^30）-bis（nitrato-O,O＇）- holmium（m） nitrate hydrate has been prepared and characterized by elemental analysis, infrared spectra, and electrospray mass spectra. Its crystal and molecular structures were determined by X-ray diffraction methods. The crystal crystallizes in the monoclinic system, space group C2/c with a = 23.737（12）, b = 14.237（7）, c = 19.801（10） A, β = 91.36（1）^o, Mr = 831.57, V = 6690（6） A^3, Z = 8, Dc = 1.651 g/cm^3, F（000） = 3344, R = 0.0482 and wR = 0.0923. The holmium ion is located in one of the compartments of the macrocyclic ligand and presents a distorted tricapped trigonal prismatic coordination geometry. The macrocycle is coordinated with two oxygen and three nitrogen atoms. Two nitrates are chelated in the opposite positions of the macrocycle, and the third one is ionic.

关键词： crystal structure lanthanide macrocycle

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Graphene nanopores toward DNA sequencing： a review of experimental aspects

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Science China Chemistry 2017年第6期60卷 721-729页

作者： Wei Chen Guo-Chang Liu Jun Ouyang Meng-Juan Gao Bo Liu Yuan-Di Zhao Britton Chance Center for biomedical photonics at wuhan national laboratory for optoelectronics-hubei bioinformatics and molecular imaging key laboratory Department of Biomedical Engineering College of Life Science and Technology Huazhong University of Science and Technology Wuhan 430074 China

Nanopores for DNA sequencing have drawn much attention due to their potentials to achieve amplification-free, low-cost, and high-throughput analysis of nuclei acids. The material configuration and fabrication of the nanopore has become one important consideration in the nanopore based DNA sequencing research. Among various materials, the newly emerged graphene has brought more opportunities to the development of sequencing technology because of its unique structures and properties. This review mainly focuses on the experimental aspects of graphene nanopore research including the nanopore fabrication methods and processes. Meanwhile, the challenges in the present graphene nanopore research including hydrophobicity, translocation velocity and noise are also addressed and discussed.

关键词： graphene nanopore DNA sequencing nanopore fabrication

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A time-coded multi-concentration microfluidic chemical waveform generator for high-throughput probing suspension single-cell signaling

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Chinese Chemical Letters 2022年第6期33卷 3091-3096页

作者： Yiran Guo Peng Chen Zhaolong Gao Yiwei Li Shunji Li Xiaojun Feng Bi-Feng Liu The key laboratory for biomedical photonics of moe at wuhan national laboratory for optoelectronics-hubei bioinformatics and molecular imaging key laboratory Systems Biology ThemeDepartment of Biomedical EngineeringCollege of Life Science and TechnologyHuazhong University of Science and TechnologyWuhan 430074China The key laboratory of Ministry of Education for Image Processing and Intelligent Control School of Artificial Intelligence and AutomationHuazhong University of Science and TechnologyWuhan 430074China

The cellular response to the complex extracellular microenvironment is highly dynamic in time and type of extracellular *** reconstructing this process and analyzing the changes in receptor conformation on the cell membrane surface and intracellular or intercellular signaling has been a major challenge in analytical chemistry and biophysical *** this paper,a time-coded multiconcentration microfluidic chemical waveform generator was developed for the dynamic signaling probing with single-cell array of high temporal resolution,high throughput,and multi-concentration combination *** on innovative microchannel structure,sophisticated external control methods and multiplexing technology,the system not only allowed for temporally sequential permutations of the four concentrations of stimuli(time code),but also generated pulsed and continuous waveforms at different frequencies in a highly controllable ***,the single-cell trap array was set up to efficiently capture cells in suspension,dramatically increasing throughput and reducing experiment preparation *** maximum frequency of the platform was 1 Hz,and one cell could be stimulated at multiple *** show the ability of the system to investigate rapid biochemical events in high throughput,pulse stimulation and continuous stimulation of different frequencies and different time codes,combined with four concentrations of histamine(HA),were generated for probing G protein-coupled receptor(GPCR)signaling in He La ***,statistical analysis was performed for the mean peak height and mean peak area of the cellular *** believe that the time-coded multi-concentration microfluidic chemical waveform generator will provide a novel strategy for analytical chemistry,biophysics,cell signaling,and individualized medicine applications.

关键词： Microfluidics Dynamic stimulation Time code High-throughput Single-cell array

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Microfluidics-based strategies for molecular diagnostics of infectious diseases

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Military Medical Research 2022年第6期9卷 727-753页

作者： Xin Wang Xian-Zhe Hong Yi-Wei Li Ying Li Jie Wang Peng Chen Bi-Feng Liu The key laboratory for biomedical photonics of moe at wuhan national laboratory for optoelectronics-hubei bioinformatics&molecular imaging key laboratory Systems Biology ThemeDepartment of Biomedical EngineeringCollegeof Life Science and TechnologyHuazhong University of Scienceand TechnologyWuhan 430074China State key laboratory of Magnetic Resonance and Atomic molecular Physics Wuhan National Laboratory for OptoelectronicsNational Centre for Magnetic Resonancein WuhanWuhan Institute of Physics and MathematicsInnovation Academy for Precision Measurement Science and TechnologyChinese Academy of Sciences-Wuhan National Laboratory for OptoelectronicsHuazhong University of Science and TechnologyWuhan 430071China Department of Radiology Canary Center at Stanford for Cancer Early DetectionSchool of MedicineStanford UniversityPalo AltoCA 94304USA

Traditional diagnostic strategies for infectious disease detection require benchtop instruments that are inappropriate for point-of-care testing(POCT). Emerging microfluidics, a highly miniaturized, automatic, and integrated technology,are a potential substitute for traditional methods in performing rapid, low-cost, accurate, and on-site *** diagnostics are widely used in microfluidic devices as the most effective approaches for pathogen *** review summarizes the latest advances in microfluidics-based molecular diagnostics for infectious diseases from academic perspectives and industrial outlooks. First, we introduce the typical on-chip nucleic acid processes,including sample preprocessing, amplification, and signal read-out. Then, four categories of microfluidic platforms are compared with respect to features, merits, and demerits. We further discuss application of the digital assay in absolute nucleic acid quantification. Both the classic and recent microfluidics-based commercial molecular diagnostic devices are summarized as proof of the current market status. Finally, we propose future directions for microfluidics-based infectious disease diagnosis.

关键词： Microfluidics molecular diagnostics Infectious disease Point-of-care testing(POCT) Digital assay

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Photostable lysosomal imaging of living cell with hyperspectral stimulated Raman scattering microscopy using a probe based on bisarylbutadiyne

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Chinese Chemical Letters 2019年第7期30卷 1393-1396页

作者： Cong Ding Yage Chen Haozheng Li Bingyao Wang Qi Wei Huajun Tang Shaokang Jia Zhiyong He Ping Wang Xiang Zhou College of Chemistry and molecular Sciences Key Laboratory of Biomedical Polymers of the Ministry of Education WuhanUniversity Wuhan 430072 China Britton Chance Center for biomedical photonics Wuhan National Laboratory for Optoelectronics Huazhong University of Science and Technology Wuhan 430074 China moe key laboratory for biomedical photonics Collaborative Innovation Center for Biomedical Engineering School of Engineering Sciences Huazhong University of Science and Technology Wuhan 430074 China

We designed a lysosome-selective Raman probe by conjugating bisphenylbutadiyne with morpholine, a well-known lysosome targeting moiety. This probe, named Lyso-BADY, has a Raman peak 28 times more intense than that of 5-ethynyl-2'-deoxyuridine. Lysosome in living cells was successfully visualized by hyperspectral stimulated Raman scattering (SRS) microscopy with this extracellular probe. Further study showed that the Raman signal of Lyso-BADY remained steady and strong even after a prolonged irradiation time. The photo-stability feature of Lyso-BADY rendered molecules of the similar structure as potentially versatile probe for continuous imaging in the future.

关键词： Raman probe SRS Cell imaging Photo-stability feature

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Coherent Raman scattering imaging of lipid metabolism in cancer

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Journal of Innovative Optical Health Sciences 2023年第3期16卷 3-12页

作者： Shuo Zhang Yexuan He Shuhua Yue key laboratory of Biomechanics and Mechanobiology(Beihang University) Ministry of EducationInstitute of Medical PhotonicsBeijing Advanced Innovation Center for Biomedical EngineeringSchool of Biological Science and Medical EngineeringBeihang UniversityBeijing 100083P.R.China moe key laboratory for biomedical photonics Wuhan National Laboratory for Optoelectronics-Huazhong University of Science and TechnologyWuhan 430074P.R.China

Cancer cells dysregulate lipid metabolism to accelerate energy production and biomolecule synthesis for rapid *** metabolism is highly dynamic and intrinsically heterogeneous at the single cell ***°uorescence microscopy has been commonly used for cancer research,bulky°uorescent probes can hardly label small lipid molecules without perturbing their biological *** a challenge can be overcome by coherent Raman scattering(CRS)microscopy,which is capable of chemically selective,highly sensitive,submicron resolution and high-speed imaging of lipid molecules in single live cells without any *** developed hyperspectral and multiplex CRS microscopy enables quantitative mapping of various lipid metabolites in *** incorporation of CRS microscopy with Raman tags greatly increases molecular selectivity based on the distinct Raman peaks well separated from the endogenous cellular *** to these unique advantages,CRS microscopy sheds new insights into the role of lipid metabolism in cancer development and *** review focuses on the latest applications of CRS microscopy in the study of lipid metabolism in cancer.

关键词： Coherent Raman scattering microscopy cancer metabolism lipid metabolism

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Divergent Projection Patterns Revealed by Reconstruction of Individual Neurons in Orbitofrontal Cortex

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Neuroscience Bulletin 2021年第4期37卷 461-477页

作者： Junjun Wang Pei Sun Xiaohua Lv Sen Jin Anan Li Jianxia Kuang Ning Li Yadong Gang Rui Guo Shaoqun Zeng Fuqiang Xu Yu-Hui Zhang Britton Chance Center for biomedical photonics Wuhan National Laboratory for Optoelectronics-Huazhong University of Science and TechnologyWuhan430074China moe key laboratory for biomedical photonics School of Engineering SciencesHuazhong University of Science and TechnologyWuhan430074China Centre for Brain Science State Key Laboratory of Magnetic Resonance and Atomic Molecular PhysicsKey Laboratory of Magnetic Resonance in Biological SystemsWuhan Institute of Physics and MathematicsCAS Centre for Excellence in Brain Science and Intelligence TechnologyChinese Academy of SciencesWuhan430071China

The orbitofrontal cortex(OFC)is involved in diverse brain functions via its extensive projections to multiple target *** is a growing understanding of the overall outputs of the OFC at the population level,but reports of the projection patterns of individual OFC neurons across different cortical layers remain ***,by combining neuronal sparse and bright labeling with a whole-brain florescence imaging system(fMOST),we obtained an uninterrupted three-dimensional whole-brain dataset and achieved the full morphological reconstruction of 25 OFC pyramidal *** compared the wholebrain projection targets of these individual OFC neurons in different cortical layers as well as in the same cortical *** found cortical layer-dependent projections characterized by divergent patterns for information *** study not only provides a structural basis for understanding the principles of laminar organizations in the OFC,but also provides clues for future functional and behavioral studies on OFC pyramidal neurons.

关键词： Orbitofrontal cortex Whole-brain imaging Morphological reconstruction Output Projection pattern

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