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The unconventional prefoldin RPB5 interactor mediates the gravitropic response by modulating cytoskeleton organization and auxin transport in Arabidopsis

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Journal of Integrative Plant Biology 2022年第10期64卷 1916-1934页

作者： Yi Yang Fang Liu Le Liu Mingyue Zhu Jinfeng Yuan Yan‐Xia Mai Jun‐Jie Zou Jie Le Yonghong Wang Klaus Palme Xugang Li Yong Wang Long Wang State Key laboratory of Crop Biology College of Life SciencesShandong Agricultural UniversityDaizong Street 61Tai'an 271018China Shandong Provincial Key laboratory of Biophysics Institute of BiophysicsDezhou UniversityDezhou 253023China Sino‐German Joint Research Center on Agricultural Biology College of Life SciencesShandong Agricultural UniversityDaizong Street 61Tai'an 271018China Faculty of Biology Institute of Biology II/Molecular Plant PhysiologyAlbert‐Ludwigs‐University of FreiburgSchänzlestrasse 1Freiburg D‐79104Germany National Key laboratory of Plant Molecular Genetics(NKLPMG) CAS Center for Excellence in Molecular Plant SciencesInstitute of Plant Physiology and Ecology(SIPPE)Chinese Academy of Sciences(CAS)Shanghai 200032China Biotechnology Research Institute Chinese Academy of Agricultural SciencesBeijing 100081China Key laboratory of Plant Molecular Physiology CAS Center for Excellence in Molecular Plant SciencesInstitute of BotanyChinese Academy of SciencesBeijing 100093China University of Chinese Academy of Sciences Beijing 100049China

Gravity-induced root curvature involves the asymmetric distribution of the phytohormone *** response depends on the concerted activities of the auxin transporters such as PIN-FORMED(PIN)proteins for auxin efflux and AUXIN RESISTANT 1(AUX1)for auxin ***,how the auxin gradient is established remains *** we identified a new mutant with a short root,strong auxin distribution in the lateral root cap and an impaired gravitropic *** causal gene encoded an Arabidopsis homolog of the human unconventional prefoldin RPB5 interactor(URI).At URI interacted with prefoldin 2(PFD2)and PFD6,twoβ-type PFD members that modulate actin and tubulin patterning in *** auxin reporter DR5_(rev):GFP showed that asymmetric auxin redistribution after gravistimulation is disordered in aturi-1 root *** with the endomembrane protein trafficking inhibitor brefeldin A indicated that recycling of the auxin transporter PIN2 is disrupted in aturi-1 roots as well as in pfd *** propose that At URI cooperates with PFDs to recycle PIN2and modulate auxin distribution.

关键词： Arabidopsis auxin cytoskeleton PIN2 prefoldin(PFD) unconventional prefoldin RPB5 interactor(URI)

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Reductive degradation of chloramphenicol by Geobacter metallireducens

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Science China(Technological Sciences) 2019年第10期62卷 1688-1694页

作者： XU HengDuo XIAO LeiLei ZHENG ShiLing ZHANG YueChao LI JiaJia LIU FangHua Key laboratory of Marine Ecology&Environmental Sciences Institute of OceanologyChinese Academy of SciencesQingdao266071China Key laboratory of Coastal Biology and Utilization Yantai Institute of Coastal Zone ResearchChinese Academy of SciencesYantai264003China laboratory for Marine Biology and Biotechnology Pilot National Laboratory for Marine Science and Technology(Qingdao)Qingdao266237China University of Chinese Academy of Sciences Beijing100049China

Geobacter metallireducens is known to be capable of removing nitroaromatic compounds via an oxidation mode. However, little attention has been paid to investigate the reductive removal of chlorinated nitroaromatic compounds by G. metallireducens. In this study, G. metallireducens was used to reduce chloramphenicol(cap), a typical chlorinated nitroaromatic antibiotic. Cyclic voltammograms and chronoamperometry highlighted a higher peak current for cap reduction by G. metallireducens compared to the control without bacteria. G. metallireducens efficiently reduced cap(20 mg/L) with acetate as the sole electron donor, and the removal efficiency reached(97.6±4.9)% within 6 d. Aromatic amine(AMCl2), AMCl(dechlorinated AMCl2) and AM(dechlorinated AMCl) were identified as reduction products by liquid chromatography-mass spectrometry. However, the removal efficiency declined to(25.0±3.6)% when the cap dosage increased to 80 mg/L. Transcriptomic analysis indicated the significant upregulation of genes related to electron transfer, such as pilus assembly protein gene(2.8 folds), NADH-quinone oxidoreductase subunit K2 gene(4.5 folds) and many c-type cytochrome genes such as cytochrome c biogenesis protein Res B(Gmet 2901, 4.6 folds), cytochrome c(Gmet 0335, 4.4 folds) and cytochrome c7(Gmet 2902, 3.4 folds). Furthermore, a gene related to chlorinated contaminant removal(Gmet 1046, 5.4 folds) was also upregulated, possibly resulting in enhanced cap reduction. This work deepened our knowledge of the bioremediation ability of G. metallireducens with respect to environmental contaminants and provided a potential strategy to treat antibiotics with electrochemically active bacteria.

关键词： chloramphenicol Geobacter metallireducens dechlorination extracellular electron transfer reduction

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Myelin-associated glycoprotein combined with chitin conduit inhibits painful neuroma formation after sciatic nerve transection

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Neural Regeneration Research 2022年第6期17卷 1343-1347页

作者： Wei Pi Ci Li Meng Zhang Wei Zhang Pei-Xun Zhang Department of Orthopedics and Trauma Peking University People’s HospitalPeking UniversityBeijingChina Key laboratory of Trauma and Neural Regeneration Peking UniversityBeijingChina National Center for Trauma Medicine BeijingChina

Studies have shown that myelin-associated glycoprotein(MAG)can inhibit axon regeneration after nerve ***,the effects of MAG on neuroma formation after peripheral nerve injury remain poorly *** this study,local injection of MAG combined with nerve cap made of chitin conduit was used to intervene with the formation of painful neuroma after sciatic nerve transfection in *** 8 weeks of combined treatment,the autotomy behaviors were reduced in rats subjected to sciatic nerve transfection,the mRNA expression of nerve growth factor,a pain marker,in the proximal nerve stump was decreased,the density of regenerated axons was decreased,the thickness of the myelin sheath was increased,and the ratio of unmyelinated to myelinated axons was ***,the percentage of collagen fiber area and the percentage of fibrosis marker alpha-smooth muscle actin positive staining area in the proximal nerve stump were *** combined treatment exhibited superior effects in these measures to chitin conduit treatment *** findings suggest that MAG combined with chitin conduit synergistically inhibits the formation of painful neuroma after sciatic nerve transection and alleviates neuropathic *** study was approved by the Animal Ethics Committee of Peking University People’s Hospital(approval No.2019PHE027)on December 5,2019.

关键词： autotomy axon chitin conduit fibrosis myelin-associated glycoprotein painful neuroma peripheral nerve regeneration

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NAD^(+)-capped RNAs are widespread in rice(Oryza sativa)and spatiotemporally modulated during development

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Science China(Life Sciences) 2022年第10期65卷 2121-2124页

作者： Haijiao Dong Xufeng Wang Cong Tan Lei Gao Jie Cui Lin Liu Beixin Mo Yongzhong Xing Yu Yu Xuemei Chen Guangdong Provincial Key laboratory for Plant Epigenetics Longhua Bioindustry and Innovation Research InstituteCollege of Life Sciences and OceanographyShenzhen UniversityShenzhen 518060China Key laboratory of Optoelectronic Devices and Systems of Ministry of Education and Guangdong Province College of Optoelectronic EngineeringShenzhen UniversityShenzhen518060China Department of Botany and Plant Sciences Institute of Integrative Genome BiologyUniversity of CaliforniaRiversideCA92521USA China National Gene Bank BGI-ShenzhenShenzhen 518120China National Key laboratory of Crop Genetic Improvement and National Center of Plant Gene Research(Wuhan) Huazhong Agricultural UniversityWuhan430070China

Dear Editor,Nicotinamide adenine dinucleotide(NAD+),a nucleotidecontaining metabolite,can be incorporated as an RNA 5′cap to form NAD^(+)-capped RNAs(NAD-RNAs),which have been identified in bacteria,yeast,mammalian cells and Arabidopsis thaliana(Hu et al.,2021).Most NAD-RNAs are m RNAs encoded by the nuclear and mitochondrial genomes。

关键词： sativa widespread

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Effect of temperature switchover on the degradation of antibiotic chloramphenicol by biocathode bioelectrochemical system

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Journal of Environmental Sciences 2014年第8期26卷 1689-1697页

作者： Deyong Kong Bin Liang Duu-Jong Lee Aijie Wang Nanqi Ren State Key laboratory of Urban Water Resource and Environment School of Municipal and Environmental EngineeringHarbin Institute of Technology Key laboratory of Environmental Biotechnology Research Center for Eco-Environmental SciencesChinese Academy of Sciences

Exposure to chloramphenicol（cap）,a chlorinated nitroaromatic antibiotic,can induce cap-resistant bacteria/genes in diverse environments. A biocathode bioelectrochemical system（BES） was applied to reduce cap under switched operational *** switching from 25 to 10°C,the cap reduction rate（kcap） and the maximum amount of the dechlorinated reduced amine product（AMCl,with no antibacterial activity） by the biocathode communities were both markedly decreased. The acetate and ethanol yield from cathodophilic microbial glucose fermentation（with release of electrons） was also reduced. Formation of the product AMCl was enhanced by the biocathode dechloridation reaction compared with that produced from pure electrochemical or microbial dechloridation processes. The electrochemical and morphological analyses of cathode biofilms demonstrated that some cathodophilic microbes could adapt to low temperature and play a key role in cap degradation. The resilient biocathode BES has a potential for the treatment of cap-containing wastewater in temperature fluctuating environments.

关键词： Chloramphenicol degradation Biocathode Dechloridation Temperature switchover

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Fast low-energy halo-to-halo transfers between Sun–planet systems

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Chinese Journal of Aeronautics 2014年第2期27卷 338-348页

作者： Shang Haibin Wang Shuai Cui Pingyuan Key laboratory of Dynamics and Control of Flight Vehicle Ministry of Education Beijing Institute of Technology

In this paper, the problem of fast low-energy halo-to-halo transfers between Sun-planet systems is discussed under ephemeris constraints. According to the structure of an invariant man- ifold, employing an invariant manifold and planetary gravity assist to save fuel consumption is ana- lyzed from the view of orbital energy. Then, a pseudo-manifold is introduced to replace the invariant manifold in such a way that more transfer opportunities are allowed. Fast escape and cap- ture can be achieved along the pseudo-manifold. Furthermore, a global searching method that is based on patched-models is proposed to find an appropriate transfer trajectory. In this searching method, the trajectory is divided into several segments that can be designed under simple dynamical models, and an analytical algorithm is developed for connecting the segments. Earth-Mars and Earth Venus halo-to-halo transfers are designed to demonstrate the proposed approach. Numerical results show that the transfers that combine the pseudo-manifolds and planetary gravity assist can offer significant fuel consumption and flight time savings over traditional transfer schemes.

关键词： Astrophysics Halo orbit Low-energy Planetary gravity assistPseudo-manifold

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Workspace,stiffness analysis and design optimization of coupled active-passive multilink cable-driven space robots for on-orbit services

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Chinese Journal of Aeronautics 2023年第2期36卷 402-416页

作者： Jianqing PENG Haoxuan WU Tianliang LIU Yu HAN School of Intelligent Systems Engineering Shenzhen Campus of Sun Yat-sen UniversityShenzhen 518109China Guangdong Provincial Key laboratory of Fire Science and Technology Guangzhou 510006China School of Mechanical Engineering and Automation Harbin Institute of TechnologyShenzhen 518055China

The use of space robots(SRs)for on-orbit services(OOSs)has been a hot research topic in recent ***,the space unstructured environment(i.e.:confined spaces,multiple obstacles,and strong radiation interference)has greatly restricted the application of *** coupled active-passive multilink cable-driven space robot(cap-MCDSR)has the characteristics of slim body,flexible movement,and electromechanical separation,which is very suitable for extreme space ***,the dynamic and stiffness modeling of cap-MCDSRs is challenging,due to the complex coupling among the active cables,passive cables,joints,and the *** deal with these problems,this paper proposes a workspace,stiffness analysis and design optimization method for such type of ***,the multi-coupling kinematics relationships among the joint,cables and the end-effector are *** on hybrid series-parallel characteristics,the improved coupled active–passive(cap)dynamic equation is ***,the maximum workspace,the maximum stiffness,and the minimum cable tension are resolved,among them,the overall stiffness is the superposition of the stiffness produced by the active and the passive ***,the workspace,the stiffness,and the cable tension are analyzed by using the nonlinear optimization method(NOPM).Finally,an 8-DOF cap-MCDSR experiment system is built to verify the proposed modeling and trajectory tracking *** proposed modeling and analysis results are very useful for practical space applications,such as designing a new cap-MCDSR,or utilizing an existing cap-MCDSR system.

关键词： Coupled active–passive(cap) Hybrid series-parallel Multilink cable-driven space robot(MCDSR) Nonlinear optimization(NOP) On-orbit services(OOSs) Robotics Space

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Ultrafast photocarrier and coherent phonon dynamics in type-II Dirac semimetal PtTe2 thin films probed by optical spectroscopy

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Photonics Research 2022年第3期10卷 653-661页

作者： Peng Suo Shengnan Yan Ruihua Pu Wenjie Zhang Di Li Jiaming Chen Jibo Fu Xian Lin Feng Miao Shi-Jun Liang Weimin Liu Guohong Ma Department of Physics Shanghai UniversityShanghai 200444China National laboratory of Solid State Microstructures School of PhysicsCollaborative Innovation Center of Advanced MicrostructuresNanjing UniversityNanjing 210093China School of Physical Science and Technology ShanghaiTech UniversityShanghai 201210China

We report the ultrafast photocarrier dynamics and coherent phonon excitation in type-II Dirac semimetal platinum ditelluride(PtTe2)thin films via femtosecond(fs)pump-probe spectroscopy at room *** analysis revealed that the incoherent electronic relaxation consists of two components:a subpicosecond fast relaxation process and a slow component with a time constant of hundreds of picoseconds(ps).Furthermore,the launch of a coherent acoustic phonon(cap)in the 20 nm film but absence in the 6.8 nm film uncovers the dominant role of temperature gradient in producing a strain *** sound velocity and Young’s modulus in the thick PtTe_(2) are determined to be 1.736 km/s and 29.5 GPa,*** addition,the coherent optical phonon(COP)with a frequency of 4.7 THz corresponding to Te atoms out-of-plane A1g vibration has been well resolved in all films,which is ascribed to displacive excitation of coherent phonon(DECP).The observation of a strong probe-wavelength dependent COP amplitude reveals the resonant feature of the optical excitation-induced atomic displacement in *** findings provide deep insight into the excitation and dynamics of cap and COP as well as the photocarriers’recovery pathway and lifetimes in PtTe_(2).Our study also demonstrates that the COP spectroscopy is a powerful tool to reveal the modulation of frequency-dependent optical constants induced by atomic vibrations,which may find applications in the fields of optoelectronics and ultrafast photonics.

关键词： coherent phonon excitation

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Molecular mechanisms of coronavirus RNA capping and methylation

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Virologica Sinica 2016年第1期31卷 3-11页

作者： Yu Chen Deyin Guo State Key laboratory of Virology College of Life Sciences Wuhan University

The 5′-cap structures of eukaryotic m RNAs are important for RNA stability, pre-m RNA splicing,m RNA export, and protein translation. Many viruses have evolved mechanisms for generating their own cap structures with methylation at the N7 position of the capped guanine and the ribose 2′-Oposition of the first nucleotide, which help viral RNAs escape recognition by the host innate immune system. The RNA genomes of coronavirus were identified to have 5′-caps in the early1980 s. However, for decades the RNA capping mechanisms of coronaviruses remained *** 2003, the outbreak of severe acute respiratory syndrome coronavirus has drawn increased attention and stimulated numerous studies on the molecular virology of coronaviruses. Here, we review the current understanding of the mechanisms adopted by coronaviruses to produce the 5′-cap structure and methylation modification of viral genomic RNAs.

关键词：病毒学临床医学药物学

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Short hairpin RNA-mediated knockdown of nuclear factor erythroid 2-like 3 exhibits tumor-suppressing effects in hepatocellular carcinoma cells

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World Journal of Gastroenterology 2019年第10期25卷 1210-1222页

作者： Miao-Mei Yu Yue-Hua Feng Lu Zheng Jun Zhang Guang-Hua Luo Comprehensive laboratory the Third Affiliated Hospital of Soochow University

BACKGROUND Hepatocellular carcinoma(HCC) is one of the most common malignant tumors with high mortality-to-incidence ratios. Nuclear factor erythroid 2-like 3(NFE2 L3), also known as NRF3, is a member of the cap ‘n' collar basic-region leucine zipper family of transcription factors. NFE2 L3 is involved in the regulation of various biological processes, whereas its role in HCC has not been *** To explore the expression and biological function of NFE2 L3 in *** We analyzed the expression of NFE2 L3 in HCC tissues and its correlation with clinicopathological parameters based on The Cancer Genome Atlas(TCGA) data portal. Short hairpin RNA(shRNA) interference technology was utilized to knock down NFE2 L3 in vitro. Cell apoptosis, clone formation, proliferation, migration,and invasion assays were used to identify the biological effects of NFE2 L3 in BEL-7404 and SMMC-7721 cells. The expression of epithelial-mesenchymal transition(EMT) markers was examined by Western blot *** TCGA analysis showed that NFE2 L3 expression was significantly positively correlated with tumor grade, T stage, and pathologic stage. The qPCR and Western blot results showed that both the mRNA and protein levels of NFE2 L3 were significantly decreased after shRNA-mediated knockdown in BEL-7404 and SMMC-7721 cells. The shRNA-mediated knockdown of NFE2 L3 could induce apoptosis and inhibit the clone formation and cell proliferation of SMMC-7721 and BEL-7404 cells. NFE2 L3 knockdown also significantly suppressed the migration, invasion, and EMT of the two cell *** Our study showed that shRNA-mediated knockdown of NFE2 L3 exhibited tumor-suppressing effects in HCC cells.

关键词： Nuclear factor erythroid 2-like 3 Hepatocellular carcinoma The Cancer Genome Atlas Short hairpin RNA Epithelial-mesenchymal transition

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