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HPTLC Analysis of multi-ingredient traditional Chinese medicine supplement

中国药理学与毒理学杂志 2015年第S1期29卷 93-94页

作者： MartinCAI SheenaGOH LengNEO FengTIAN School of Applied Science Temasek Polytechnic Singapore 529757

OBJECTIVE Analysis of traditional Chinese medicinal(TCM)supplements has always been a laborious task,particularly in the case of multi-ingredient ***,herbal extracts are analyzed using one or few markers *** the recent years,however,pharmaceutical companies are introducing health supplements of TCM ingredients to cater to the needs of consumers in the fast-paced society in this *** such,new problems arise in the aspects of composition identification as well as quality *** most cases of products or supplements formulated with multiple TCM herbs,the chemical composition and nature of each raw material differs greatly from the others in the *** results in a requirement for individual analytical processes in order to identify the marker compounds in the various *** aim of this study is to establish a rapid and low cost analytical approach using high performance thin layer chromatography(HPTLC)for the analysis of 3 key components of the LAC Liver Protector,a supplement with TCM formulation aimed at improving liver *** The TCM herbs were extracted with methods derived from Chinese Pharmacopeia *** herbal extracts were then applied to HPTLC plates using CAMAG Linomat 5 and developed using fully saturated twin-trough chromatographic *** developed plates were derivatized and documented with CAMAG TLC *** images were processed with CAMAG winCATS software to calculate the Rf values and confirm the presence of key constituents of the *** The 3 key components of LAC Liver Protector product,namely Radix notoginseng,Rhizoma alismatis and Radix paeoniae alba/rubra were identified in all 6 batches of products *** With the increasing trend of small and medium-sized enterprises(SMEs)bringing natural products and health supplements into the market,it is crucial that the qualities of both raw materials and end products be well-assured for the protection of

关键词： TLC HPTLC Radix notoginseng Rhizoma alismatis Radi

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Qualitative and quantitative analysis of Arnebiae Radix and Dictamni Cortex and efficacy study of herbal extracts on allergic contact dermatitis using 3D human reconstructed epidermis

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Chinese Herbal Medicines 2021年第4期13卷 556-564页

作者： Huan Li Esther Lim Gladys Ang Zhi-qing Lim Martin Hui Cai Jo-anne Loh Celine Ng Peijia Seetoh Edmund Tian Lay Beng Goh Centre of Innovation for Complementary Health Product School of Applied ScienceTemasek PolytechnicSingapore 529757Singapore Translational Research Project School of Applied ScienceTemasek PolytechnicSingapore 529757Singapore

Objective:To evaluate the quality of Arnebiae Radix(AR)and Dictamni Cortex(DC)and study the efficacy of herbal extracts of these two herbs on the treatment of allergic contact dermatitis(ACD).Methods:Qualitative and quantitative analysis of effective components was performed using High Performance Thin Layer Chromatography(HPTLC),High Performance Liquid Chromatography(HPLC),and HPLC-Quadrupole Time of Flight-Mass Spectrometry(HPLC-QTOF-MS).In vitro allergic ACD 3D model was established by incubating 3D reconstructed human epidermis(RHE)with skin sensitizer,potassium dichromate.A total of 65 gene expression that were associated with ACD,which included 24 antioxidant responsive element(ARE)and 41 SENS-IS genes were quantified by q *** than or equal to 10 ARE genes and 18 SENN-IS genes were induced by 1.3-fold,demonstrating the successful establishment of in vitro ACD *** extracts of AR and DC were applied on the in vitro ACD model to study the ***:Batch 3 of AR and batch 2 of DC showed presence of all active ingredients with the highest *** ingredients of the herbs were extracted using a special oil and formulated into herbal oil *** herbal oil extracts were able to down regulate the induced genes in the in-vitro ACD skin model,bringing the tissue back to homeostatic ***:The oil extracts showed the potent efficacy of using AR and DC in ACD *** combination study will be done to optimize the formulation ratio which will be developed into a topical cream.

关键词： allergic contact dermatitis Arnebiae Radix Chinese medicine Dictamni Cortex 3D reconstructed epidermis quality control

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Molecular rotors as a class of generally highly active ion transporters

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Science China Chemistry 2021年第12期64卷 2154-2160页

作者： Jie Shen Joan Jia Ying Han Ruijuan Ye Huaqiang Zeng Frontier Research Center for Multidisciplinary Sciences School of Chemistry and Chemical EngineeringNorthwestern Polytechnical UniversityXi’an710072China temasek polytechnic 21 Tampines Avenue 1Singapore529757Singapore

We describe here a class of unconventional ion transporters,molecular rotors that transport ions through a rotating function rather than via traditional carrier or channel *** macroscopic rotors,these molecular rotors consist of three modularly tunable components,i.e.,a membrane-anchoring stator,a crown ether-containing rotator for ion binding and transport,and a triple bond-based axle that allows the rotator to freely rotate around the stator in the lipid *** bilayer experiments reveal the generally high ability of all molecular rotors in promoting the highly efficient transmembrane K^(+)flux(EC50 values=0.49-1.37 mol%relative to lipid).While molecular rotors differing only in the ion-binding unit exhibit similar ion transport activities,those differing in the rotator’s length display activity differences by up to 174%.

关键词： supramolecular chemistry molecular machines artificial membrane transporters molecular rotors K+transporters

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Towards a Medium/High Load-Bearing Scaffold Fabrication System

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Tsinghua Science and Technology 2009年第S1期14卷 13-19页

作者： GIBSON Ian SAVALANI Monica M LAM Christopher X F OLKOWSKI Radoslow EKAPUTRA Andrew K TAN Kim Cheng HUTMACHER Dietmar W Faculty of Engineering National University of Singapore Department of Biophysics and Human Physiology Medical University of Warsaw Warsaw 02-091 Poland temasek Engineering School Temasek Polytechnic Institute of Health and Biomedical Innovation Queensland University of Technology Brisbane QL-4000 Australia

This paper describes fabrication of scaffolds for load-bearing applications, with primary consideration from the manufacturing perspective. An extrusion device, inspired by the FDM process, was used to create scaffolds from a variety of different polymeric materials and mixtures. The effectiveness of these scaffolds to host cells for bone regeneration has been investigated. This ongoing work has generated significant insight into the future direction of research and the possibilities of developing scaffolds for medium/high load-bearing applications.

关键词： tissue engineering load-bearing bone engineering scaffolds

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Hybrid liposome-erythrocyte drug delivery system for tumor therapy with enhanced targeting and blood circulation

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Regenerative Biomaterials 2023年第1期10卷 1021-1032页

作者： Kehui Zhu Yingcan Xu Rui Zhong Wanjing Li Hong Wang Yee Shan Wong Subramanian Venkatraman Jiaxin Liu Ye Cao Institute of Blood Transfusion Chinese Academy of Medical Sciences&Peking Union Medical CollegeChengduChina Biomedical Engineering School of EngineeringTemasek PolytechnicSingaporeSingapore School of Materials Science and Engineering National University of SingaporeSingaporeSingapore

Liposome,a widely used drug delivery system(DDS),still shows several disadvantages such as dominant clearance by liver and poor target organ *** overcome the drawbacks of liposomes,we developed a novel red blood cell(RBC)-liposome combined DDS to modulate the tumor accumulation and extend the blood circulation life of the existing liposomal ***,RBCs,an ideal natural carrier DDS,were utilized to carry liposomes and avoid them undergo the fast clearance in the *** this study,liposomes could either absorbed onto RBCs’surface or fuse with RBCs’membrane by merely altering the interaction time at 37℃,while the interaction between liposome and RBCs would not affect RBCs’*** the in vivo antitumor therapeutic efficacy study,1,2-dipalmitoyl-sn-glycero-3-phosphocholine(DPPC)liposomes attached onto RBCs’surfaces exhibited lung targeting effect(via RBC-hitchhiking approach)and reduced clearance in the liver,while DPPC liposomes fused with RBCs had prolong blood circulation up to 48 h and no enrichment in any ***,20 mol%of DPPC liposomes were replaced with pH-sensitive phospholipid 1,2-dioleoyl-Sn-glycero-3-phosphoethanolamine(DOPE)as it could respond to the low pH tumor microenvironment and then accumulate in the *** DOPE attached/fusion RBCs showed partial enrichment in lung and about 5-8%tumor accumulation,which were significantly higher than(about 0.7%)the conventional liposomal ***,RBC-liposome composite DDS is able to improve the liposomal tumor accumulation and blood circulation and shows the clinical application promises of using autologous RBCs for antitumor therapy.

关键词： liposomes erythrocyte RBC-hitchhiking blood circulation target delivery

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In vitro and in vivoimmunomodulatory activities of polysaccharides purified from four species of Dendrobium

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中国药理学与毒理学杂志 2015年第S1期29卷 64-64页

作者： Lei SHI Hui CAI Seng Poon ONG Feng TIAN School of Applied Science Temasek Polytechnic

OBJECTIVE To investigate the isolation,extraction,purification,and immunomodulatory activities in vitro and in vivo of polysaccharides from four different species of Dendrobiumherbs widely used in *** To apply the classic method of water extraction and ethanol precipitation and the gel column chromatography technique,the polysaccharides from four species of Dendrobium herbs(***,***,***,***)were *** polysaccharides were tested first on RAW 264.7 cell line with MTT method to test the effect of polysaccharides on *** two polysaccharides from *** were found to promote proliferation of the cells,and were then tested on mice splenocyte primary cell *** same polysaccharides were also injected to female BALB/c mice at various doses for 21 dto see whether they would have affected the organs,especially the livers,spleens and *** Out of the 4 Dendrobiumspecies tested,it was found that polysaccharides from *** be most supportive of the proliferation of macrophages in a dose-dependent manner,with increasing effect with the increasing doses *** from *** *** were also found to act similarly albeit to a lesser *** two *** polysaccharides were also found to greatly enhance the proliferation of mouse splenocytes in vitro and growth of the spleen in *** The immunomodulatory activities in vitro and in vivo of polysaccharides from four species of Dendrobium herbs were *** diverse chemical characters might contribute to their varied bioactivity,which should be further *** addition,it was suggested that the polysaccharides from *** could be potential immunostimulants used in the healthcare and food industry.

关键词： polysaccharides Dendrobium RAW 264.7macrophages im

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Cytogenetic and molecular aberrations of multiple myeloma patients： a single-center study in Singapore

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Chinese Medical Journal 2013年第10期126卷 1872-1877页

作者： LIM Alvin Soon Tiong LIM Tse Hui SEE Karen Hsu Shien NG Yit Jun TAN Yu Min CHO0 Natasha Swee Lian LIM Sherry Xin Er YEE Yenny LAU Lai Ching TIEN Sim Leng Kumar Sathish TAN Daryl Chen Lung Cytogenetics Laboratory Department of Pathology SingaporeGeneral Hospital Singapore School of Biological Sciences Nanyang Technological UniversitySingapore temasek Applied Science School Temasek Polytechnic Singapore Department of Haematology Singapore General HospitalSingapore

Background Much is known about the cytogenetic lesions that characterize multiple myeloma （MM） patients from the USA, Europe, and East Asia. However, little has been published about the disease among Southeast Asians. The aim of this study was to determine the chromosomal abnormalities of MM patients in our Singapore population. Methods Forty-five newly-diagnosed, morphologically confirmed patients comprising 18 males and 27 females, aged 46-84 years （median 65 years） were investigated by karyotyping and fluorescence in situ hybridization （FISH）. FISH employing standard panel probes and lp36/lq21 and 6q21/15q22 probes was performed on diagnostic bone marrow samples. Results Thirty-four cases （75.6%） had karyotypic abnormalities. Including FISH, a total detection rate of 91.1% was at- tained. Numerical and complex structural aberrations were common to both hyperdiploid and non-hyperdiploid patients. Numerical gains of several recurring chromosomes were frequent among hyperdiploid patients while structural rear- rangements of several chromosomes including 8q24.1 and 14q32 characterized non-hyperdiploid patients. With FISH, immunoglobulin heavy chain （IGH） gene rearrangements, especially fibroblast growth factor receptor 3 （FGFR3）/IGH and RB1 deletion/monosomy 13 were the most common abnormalities （43.4%）. Amplification lq21 was 10 times more frequent （42.5%） than del（1p36） and del（6q21）. Conclusions We have successfully reported the comprehensive cytogenetic profiling of a cohort of newly-diagnosed myeloma patients in our population. This study indicates that the genetic and cytogenetic abnormalities, and their frequencies, in our study group are generally similar to other populations.

关键词： amp(l q21), del(13q) fluorescence in situ hybridization, immunoglobulin heavy chain, Southeast Asia

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Development of SC-10: A psychometrically equivalent Singapore Mandarin disyllabic word list for clinical speech audiometry use

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World Journal of Otorhinolaryngology-Head and Neck Surgery 2021年第3期7卷 247-256页

作者： Gary Jek Chong Lee Steven Lock Hey Lee Audiology Ng Teng Fong General HospitalSingapore temasek Skillsfuture Academy Temasek PolytechnicSingapore Otolaryngology Changi General HospitalSingapore

Objective::The aim of this study is to develop and evaluate a set of psychometrically equivalent disyllabic wordlist(SC-10)in Singapore Mandarin for clinical *** design::A preliminary set of 1000 words were obtained from a dictionary of frequently used words by Singapore *** native judges rate the familiarity level of each *** is followed by a face-to-face public survey to rank the shortlisted word set from most to least *** final 108 disyllabic words were recorded by a native female talker.20 normal hearing subjects were used to obtain the percentage of correct word recognition at 24 intensity levels(-10 dB HL to 26 dB HL in 2 dB increment).Psychometric function slopes were calculated for each word.100 words were eventually chosen and assigned into ten 10-word lists based on a psychometric balancing *** digital adjustments were made to the intensity of each wordlist to improve their auditory ***::The calculated regression slopes in the psychometric functions for the ten lists are between 8.0 and 9.8%/*** factor ANOVA analysis showed no significant difference in both the mean intensity required to obtain 50%recognition score(f=0.109,df=9,p=0.999)and the slopes of the psychometric functions(f=0.078,df=9,p=0.999)between the ten word *** validation on 25 normal hearing participants(PTA=11.0 dB HL,SD=4.3)showed a mean speech recognition threshold(SRT)of 9.3 dB HL(SD=3.5)and regression slope of 8.395%/*** regression analysis showed a positive correlation(r^(2)=0.923)between presentation level and word recognition score(WRS).The difference between PTA and SRT of each subject all fall within the clinically acceptable difference of 10 dB ***-retest reliability,carried out on 20 subjects at three levels(SRT-5,SRT,and SRT+5 dB),showed no significance difference between word recognition score when the same participant is tested again at the same intensity level using a different ***

关键词： Speech audiometry Word recognition score Disyllabic word list Mandarin Chinese Singapore SC-10

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Proton Exchange Membrane Fuel Cell Modeling Based on Seeker Optimization Algorithm

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Journal of Southwest Jiaotong University(English Edition) 2008年第2期16卷 120-124页

作者：李奇戴朝华陈维荣贾俊波韩明 School of Electric Engineering Southwest Jiaotong University School of Engineering Temasek PolytechnicSingapore 529757 School of Electrical and Electronic EngineeringNanyang Technological UniversitySingapore 639798 School of Engineering Temasek PolytechnicSingapore 529757

Seeker optimization algorithm （SOA） has applications in continuous space of swarm intelligence. In the fields of proton exchange membrane fuel cell （PEMFC） modeling, SOA was proposed to research a set of optimized parameters in PEMFC polarization curve model. Experimental result showed that the mean square error of the optimization modeling strategy was only 6.9 × 10^-23. Hence, the optimization model could fit the experiment data with high precision.

关键词： PEMFC modeling' Seeker optimization algorithm Parameter optimization

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Penetration of Topical Glucosamine Sulfate into the Synovial Fluid of Patients with Knee Osteoarthritis: A Nonrandomized, Open-Label, Single Dose, Bioavailability Study

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Journal of Biosciences and Medicines 2019年第11期7卷 76-90页

作者： Matthew Kong Khadijah Binte Hashim Phoebe Lin Gaë lle Coestesquis Aiyun Xu Frederick Lebes Choon Meng Ting School of Applied Science Temasek Polytechnic Singapore Lynk Biotechnologies Pte. Ltd. Singapore T & T Family Health Clinic & Surgery Singapore

Objective: This study aims to show that a proprietary topical cream can deliver glucosamine through the skin into the synovial fluid of osteoarthritic patients. This cream contains 10% w/w glucosamine sulfate. It also aims to determine the endogenous level of glucosamine in the synovial fluid of these patients. Therapeutic effectiveness of glucosamine is not addressed in this study. Design: This phase IV, open-label, nonrandomized study enrolled 240 patients. Participants from the Test group received a single dose treatment (2 g of cream), and synovial fluid samples were collected 1 - 3 hours post-treatment. Patients from the Control group were not subjected to any treatment but their synovial fluid was also sampled to establish a glucosamine concentration baseline for Time-0 (T0). Glucosamine concentrations were determined by HPLC analysis. Results: The mean glucosamine concentration in the synovial fluid of patients from the Test group (100.56 ng/ml, 95% CI 66.36 - 134.76, n = 117) was higher than in the Control group (17.83 ng/ml, 95% CI 7.42 - 28.24, n = 117) resulting in a significant between-group difference (p Conclusion: The results suggest that glucosamine can be topically delivered across the human skin into the synovial fluid using a proper vehicle. This suggests that other water-soluble molecules could similarly be delivered transdermally, alleviating the need for oral delivery in cases where oral administration is difficult, or when harmful side effects could ensue.

关键词： Osteoarthritis of the Knee Transdermal Delivery Glucosamine Synovial Fluid Joints

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