In vitro and in vivoimmunomodulatory activities of polysaccharides purified from four species of Dendrobium

作     者：Lei SHI Hui CAI Seng Poon ONG Feng TIAN 

作者机构：School of Applied ScienceTemasek Polytechnic 

出 版 物：《中国药理学与毒理学杂志》 (Chinese Journal of Pharmacology and Toxicology)

年 卷 期：2015年第29卷第S1期

页      面：64-64页

核心收录：

学科分类：1008[医学-中药学(可授医学、理学学位)] 1006[医学-中西医结合] 100602[医学-中西医结合临床] 10[医学] 

基　　金：The project supported by The Ministry of Education Translational R&D and Innovation Fund(MOE2012-TIF-1-G-065) Singapore 

主　　题：polysaccharides Dendrobium RAW 264.7macrophages im 

摘      要：OBJECTIVE To investigate the isolation,extraction,purification,and immunomodulatory activities in vitro and in vivo of polysaccharides from four different species of Dendrobiumherbs widely used in *** To apply the classic method of water extraction and ethanol precipitation and the gel column chromatography technique,the polysaccharides from four species of Dendrobium herbs(***,***,***,***)were *** polysaccharides were tested first on RAW 264.7 cell line with MTT method to test the effect of polysaccharides on *** two polysaccharides from *** were found to promote proliferation of the cells,and were then tested on mice splenocyte primary cell *** same polysaccharides were also injected to female BALB/c mice at various doses for 21 dto see whether they would have affected the organs,especially the livers,spleens and *** Out of the 4 Dendrobiumspecies tested,it was found that polysaccharides from *** be most supportive of the proliferation of macrophages in a dose-dependent manner,with increasing effect with the increasing doses *** from *** *** were also found to act similarly albeit to a lesser *** two *** polysaccharides were also found to greatly enhance the proliferation of mouse splenocytes in vitro and growth of the spleen in *** The immunomodulatory activities in vitro and in vivo of polysaccharides from four species of Dendrobium herbs were *** diverse chemical characters might contribute to their varied bioactivity,which should be further *** addition,it was suggested that the polysaccharides from *** could be potential immunostimulants used in the healthcare and food industry.