Qualitative and quantitative analysis of Arnebiae Radix and Dictamni Cortex and efficacy study of herbal extracts on allergic contact dermatitis using 3D human reconstructed epidermis

作     者：Huan Li Esther Lim Gladys Ang Zhi-qing Lim Martin Hui Cai Jo-anne Loh Celine Ng Peijia Seetoh Edmund Tian Lay Beng Goh Huan Li;Esther Lim;Gladys Ang;Zhi-qing Lim;Martin Hui Cai;Jo-anne Loh;Celine Ng;Peijia Seetoh;Edmund Tian;Lay Beng Goh

作者机构：Centre of Innovation for Complementary Health ProductSchool of Applied ScienceTemasek PolytechnicSingapore 529757Singapore Translational Research ProjectSchool of Applied ScienceTemasek PolytechnicSingapore 529757Singapore 

出 版 物：《Chinese Herbal Medicines》 (中草药（英文版）)

年 卷 期：2021年第13卷第4期

页      面：556-564页

学科分类：1008[医学-中药学(可授医学、理学学位)] 1007[医学-药学(可授医学、理学学位)] 1006[医学-中西医结合] 100602[医学-中西医结合临床] 10[医学] 

基　　金：supported by 2019 Translational R&D and Innovation Fund Grant from Ministry of Education in Singapore 

主　　题：allergic contact dermatitis Arnebiae Radix Chinese medicine Dictamni Cortex 3D reconstructed epidermis quality control 

摘      要：Objective:To evaluate the quality of Arnebiae Radix(AR)and Dictamni Cortex(DC)and study the efficacy of herbal extracts of these two herbs on the treatment of allergic contact dermatitis(ACD).Methods:Qualitative and quantitative analysis of effective components was performed using High Performance Thin Layer Chromatography(HPTLC),High Performance Liquid Chromatography(HPLC),and HPLC-Quadrupole Time of Flight-Mass Spectrometry(HPLC-QTOF-MS).In vitro allergic ACD 3D model was established by incubating 3D reconstructed human epidermis(RHE)with skin sensitizer,potassium dichromate.A total of 65 gene expression that were associated with ACD,which included 24 antioxidant responsive element(ARE)and 41 SENS-IS genes were quantified by q *** than or equal to 10 ARE genes and 18 SENN-IS genes were induced by 1.3-fold,demonstrating the successful establishment of in vitro ACD *** extracts of AR and DC were applied on the in vitro ACD model to study the ***:Batch 3 of AR and batch 2 of DC showed presence of all active ingredients with the highest *** ingredients of the herbs were extracted using a special oil and formulated into herbal oil *** herbal oil extracts were able to down regulate the induced genes in the in-vitro ACD skin model,bringing the tissue back to homeostatic ***:The oil extracts showed the potent efficacy of using AR and DC in ACD *** combination study will be done to optimize the formulation ratio which will be developed into a topical cream.