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Plasticity of Regulation of Mannitol Phosphotransferase System Operon by CRP-cAMP Complex in Vibrio cholerae

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Biomedical and Environmental Sciences 2013年第10期26卷 831-840页

作者： ZHOU Yan Yan ZHANG Hong Zhi LIANG Wei Li ZHANG Li Juan ZHU Jun KAN Biao State Key Laboratory for communicable disease Prevention and Control National Institute for Communicable Disease Control and PreventionChinese Center for Disease Control and Prevention department of Microbiology Perelman School of MedicineUniversity of Pennsylvania

Objective The complex of the cyclic AMP receptor protein （CRP） and cAMP is an important transcriptional regulator of numerous genes in prokaryotes. The transport of mannitol through the phosphotransferase systems （PTS） is regulated by the CRP-cAMP complex. The aim of the study is to investigate how the CRP-cAMP complex acting on the mannitol PTS operon mtl of the Vibrio cholerae El Tot biotype. Methods The crp mutant strain was generated by homologous recombination to assess the need of CRP to activate the mannitol PTS operon of V. choleroe El Tor. Electrophoretic mobility shift assays （EMSA） and the reporter plasmid pBBRlux were used to confirm the role that the CRP-cAMP complex playing on the mannitol PTS operon intl. Results In this study, we confirmed that CRP is strictly needed for the activation of the mtl operon. We further experimentally identified five CRP binding sites within the promoter region upstream of the mannitol PTS operon mtl of the Vibrio cholerae El Tor biotype and found that these sites display different affinities for CRP and provide different contributions to the activation of the operon. Conclusion The five binding sites collectively confer the strong activation of mannitol transfer by CRP in V. choleroe, indicating an elaborate and subtle CRP activation mechanism.

关键词： Cyclic AMP receptor protein CRP-cAMP complex CRP-binding sites Vibrio cholerae

Willingness to pay for colorectal cancer screening in Guangzhou

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World Journal of Gastroenterology 2018年第41期24卷 4708-4715页

作者： Qin Zhou Yan Li Hua-Zhang Liu Ying-Ru Liang Guo-Zhen Lin department of Non-communicable Chronic disease Control and Prevention Guangzhou Center for Disease Control and Prevention

AIM To measure the willingness to pay for colorectal cancer screening in Guangzhou, and to identify those factors associated with it. METHODS A face-to-face questionnaire survey for pre-screening population from free and non-free colonoscopy districts was used to collect information on demographic characteristics, health behaviours, the intention of the cancer screenings and willingness to pay for colorectal cancer screening. A total of 1243 participants who took part in the pre-screening for colorectal cancer in Guangzhou were collected in the study. Categorical data were compared using the χ~2 test to analyse significant differences. Non-conditional logistic regression and multi-class logistic regression were also performed for multivariate analysis and to estimate the odds *** The percentage of participants willing to pay for colorectal cancer screening was 91.7%. "Unnecessary" was the dominant reason that participants gave for their unwillingness, accounting for 63.1%. Of those who were willing to pay, 29.2%, 20.7%, 14.8%, 13.0% and 22.4% of participants were willing to pay less than $100, $100-$199, $200-299, $300-$399 and more than $400, respectively. Non-logistic regression analysis showed that respondents who were male, had a high level of education, were from the family with more children/older to raise, and accepted colorectal cancer screening were willing to pay for this screening. Multi-class logistic regression analysis showed that respondents with higher annual household income per capita, from government and private enterprises, government agency/institution and peasants, and less family medical expenditure were willing to pay *** Willingness to pay for colorectal cancer screening in Guangzhou is high, but the amount of willing to pay is not much.

关键词： Colorectal cancer Screening Willingness to pay Guangzhou Factor

Sanitary Evaluation of Rural Water Supply Projects—China,2020

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China CDC weekly 2022年第35期4卷 775-778页

作者： Yunting Xia Hongxing Li Chuanlong Xiong Rong Zhang Shandong Provincial Key Laboratory of communicable disease Control and Prevention Department of Communicable Disease Control and PreventionShandong Center for Disease Control and PreventionJinanShandongChina

Summary What is already known about this topic?Over the last decade,the centralized water quality and supply have been substantially enhanced in rural ***,compared with the urban water supply,most of the rural Water Supply Projects(WaSPs)are small in scale,simple in engineering facilities,and poor in *** is added by this report?Most of the rural WaSPs have been basically guaranteed sustainable sources of *** measures should be taken to improve water disinfection effects,water losses control,and operational and maintenance *** WaSPs with water supply<3,000 m3/d need to be paid more *** are the implications for public health practice?Laws and regulations requiring rural WaSPs to carry out a sanitary evaluation should be *** should use tools such as World Health Organization Water Safety Plans to identify and control risks.

关键词： Rural rural carry

Epidemiological Characteristics of Human Rabies——Shandong Province,China,2010-2020

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China CDC weekly 2022年第35期4卷 793-797页

作者： Xiaomei Zhang Xueying Tian Bo Pang Zhiqiang Wang Wenji Zhai Xiaolin Jiang Zengqiang Kou Shujun Ding Xianjun Wang Shandong Provincial Key Laboratory of communicable disease Control and Prevention Department of Communicable Disease Control and PreventionShandong Center for Disease Control and PreventionJinanShandongChina

Introduction:Rabies is a viral zoonotic disease that causes progressive and fatal inflammation in the *** has caused more than 5,000 human deaths in Shandong Province since *** study aimed to analyze the epidemiological characteristics of human rabies in Shandong Province from 2010 to 2020 and to provide a scientific basis for policy ***:The data of reported human rabies cases from 2010 to 2020 were obtained from China’s National Notifiable disease Reporting System,and data related to exposure and post-exposure prophylaxis(PEP)of the cases were acquired through case ***:A total of 414 human rabies cases were reported in Shandong Province from 2010 to *** of the 414 total cases,87.20%were primarily farmers;83.10%were over 40 years old.70.29%(265/377)belonged to categoryⅢexposure;and 96.67%(377/390)were exposed to the virus through infected *** vaccine inoculation rate of these cases after exposure was only 8.85%;1.03%(4/390)had been vaccinated with rabies immunoglobulin,developing the disease 11 to 13 days after categoryⅢ***:Dogs were still the primary animal *** rabies patients died due to no or inadequate post-exposure *** inoculation rates for dogs should reach the target of 70%as soon as *** departments should improve the accessibility and quality of PEP;and improve the health literacy of the elderly in rural areas.

关键词： Shandong category policy

Genetic Diversity and Drug Susceptibility of Mycobacterium tuberculosis Isolates in a Remote Mountain Area of China

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Biomedical and Environmental Sciences 2018年第5期31卷 351-362页

作者： ma ai jing wang sheng fen fan jia le zhao bing he guang xue zhao yan lin national institute for communicable disease control and prevention chinese center for disease control and preventionBeijing 102206China national center for tb control and prevention chinese center for disease control and preventionBeijing 102206China science and technology department chinese center for disease control and preventionBeijing 102206China

Objective We determined the genetic diversity of Mycobacterium tuberculosis（Mtb） in a remote mountainous area of southwest China and evaluated the resolving ability of single nucleotide polymorphism（SNP） genotyping combined with variable number tandem repeat（VNTR） genotyping for Beijing family strains in association with drug resistance *** Three hundred thirty-one Mtb strains were isolated from patients living in mountainous regions of southwest China,and 8-loci SNP,VNTR-15 genotyping assays,and drug susceptibility testing of 9 drugs were *** A total of 183 [55.29%（183/331）] strains were classified into the Beijing *** the 183 strains,111（60.66%） were defined as modern Beijing *** most predominant modern Beijing sub-lineage and ancient Beijing sub-lineage were Bmyc10 [39.34%（72/183）] and Bmyc25 [20.77%（38/183）],*** the isolates,19.64%（65/331） were resistant to at least 1 of the 9 anti-tb drugs and 17 [4.98%（17/331）] Mtb isolates were multi-drug resistant tuberculosis（MDR-tb）.Two hundred sixty-one isolates showed a clustering rate of 14.18%（37/261） and a discriminatory index of *** Beijing lineage exhibited a significantly higher prevalence of MDR-tb,as well as resistance to isoniazid（INH）,rifampin（RIF）,and para-aminosalicylic acid（PAS） when analyzed independently（P = 0.005,P = 0.017,P = 0.014,and P = 0.006 respectively）.The Beijing lineage was not associated with genetic clustering or resistance to any *** addition,genetic clustering was not associated with drug *** Mtb strains demonstrate high genetic diversity in remote mountainous areas of southwest *** strains,especially modern Beijing strains,are predominant in remote mountainous area of *** combination of 8-loci SNPs and VNTR-15 genotyping is a useful tool to study the molecular epidemiology of Mtb strains in this area.

关键词： Mycobacterium tuberculosis Beijing strains Variable number tandem repeat (VNTR) Singlenucleotide polymorphism (SNP)

Successful isolation of Helicobacter pylori after prolonged incubation from a patient with failed eradication therapy

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World Journal of Gastroenterology 2009年第12期15卷 1528-1529页

作者： Yan Yin Li-Hua He Jian-Zhong Zhang department of Diagnosis National Institute for-Communicable Disease Control and Prevention Chinese Center for Disease Control and Prevention Beijing 102206 China

Helicobacter pylori(H pylori),a gastric pathogen,is a major cause of chronic gastritis and peptic ulcer disease,and is an important risk factor for the development of gastric *** of the bacterium from gastric biopsy is essential for the determination of drug resistance of H ***,the isolation rates of H pylori from infected individuals vary from 23.5%to 97% due to a number of factors such as biopsy preparation,cultural environment,medium and the method *** the present case,we found that a prolonged incubation period of up to 19 d allowed successful isolation of H pylori from a patient who received triple therapy that failed to eradicate the bacterium.

关键词： Helicobacter pylori Isolation Eradication

Investigation of anaplasmosis in Yiyuan County,Shandong Province,China

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Asian Pacific Journal of Tropical Medicine 2011年第7期4卷 568-572页

作者： Lijuan Zhang Feng Cui Lingling Wang Lingling Zhang Jingshan Zhang Shiwen Wang Shuxia Yang department of Rickettsiology and Anaplasmosis National Institute of Communicable Disease Control and PreventionChina CDC Zibo Center for disease Control & Prevention

Objective:To investigate the situation of anaplasmosis in Yiyuan *** Province. Methods:A total of 26 blood samples from febrile patients suspected of anaplasmosis,48 blood samples from healthy farmers,8 from dogs,and 10 from goats and 170 ticks were collected in the same area during 2005-2007,and detected by serological and molecular ***: Eight confirmed cases and 6 probable cases were determined using serologic and molecular *** seroprevalence of Anaplasma phagocytophilum(***) was 26.7% in healthy *** out of 10 sheep samples and 7 out of 8 dog samples reacted positively to the *** *** amplification and sequencing of the 16SrRNA of,4. phagocytophilum gene showed that some samples from patients,goats and ticks were 100% *** seroprevalence of Rickettsia typhi was 22.9%,Orientia tsutsugamushi 6.3%, Rickettsia sibirica 27.1%,Coxiella burnetii 18.8%,Bartonella henselae 31.3%,and Borrelia burgdorferi 41.6%.Conclusions:It is important to make differential diagnosis of febrile patients and to apply treatment with specific *** is needed to enforce essential prevention and control measures including tick control and to improve sanitation conditions.

关键词： Anaplasma phagocytophilum Field investigation Rickettsia Prevalence Yiyuan County Shandong Province China

Identification of Orientia tsutsugamushi,spotted fever group and typhus group Rickettsia by duplex and nested PCR methods

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Asian Pacific Journal of Tropical Medicine 2008年第4期1卷 1-8页

作者： M-C Luan D-Z Yu L Tang L-J Zhang department of Rickettsiology National Institute of Communicable Disease Control and PreventionChina CDC department of Microorganism Dalian Center for Disease Control and Prevention department of Microecology Dalian medical university department of Rickettsiology National Institute of Communicable Disease Control and PreventionChina.CDC

Objective:To identify members of genera of rickettsia and *** ***:Rapid and duplex and nested PCR methods have been established by designing primers based on the conserved regions of heat shock protein GroEL gene.345 mouse viscera samples including liver,spleen and kidney,96 Xenopsylla cheopis and 32 chiggers collected from Hongta areas of Yuxi city,Yunnan province were tested by the new PCR ***:The result of the study showed that the new PCR methods could identify most members of genera -Rickettsia and Orientia simultaneously with 100%specificity and its sensitivity could test one copy per *** results of detection prevalence of rickettsioses in mouse,flea and mites DNA samples showed that the total rickettsia infection rate in mouse was 34.78%(120/345).The total infection rates in ***,O.t Karp and *** of mouse samples were 28.12%(97/345),19.71%(68/345) and O. 29%(1/345) ***-infection rates in *** and 0.t Karp of mouse samples were 13.33%(46/ 345).O.t Karp type has been the main epidemic strain in these ***:We concluded that this PCR method could be used to detect multi-genera rickettsia *** evidences provided in this and previous studies strongly support that Hongta areas of Yuxi city are a natural focus for typhus and scrub typhus with the common occurrence of their confection.

关键词： Rickettsia,GroEL gene Duplex and nested PCR

Characterization of Mutations in Genes Related to Rifampicin and Isoniazid Resistance in Multidrug-resistant Mycobacterium tuberculosis Strains from Hangzhou,China

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Biomedical and Environmental Sciences 2023年第9期36卷 869-873页

作者： HUANG Yin Yan XIE Li WU Yi Fei JIA Qing Jun CHENG Qing Lin LI Qing Chun AI Li Yun BAI Xue Xin department of tb Control and Prevention Hangzhou Center for Disease Control and PreventionHangzhou 310021ZhejiangChina

Tuberculosis(tb),caused by the Mycobacterium tuberculosis complex(MtbC),is the 13^(th)leading cause of death and the second leading cause of infectious killer after COVID-19(above HIV and AIDS),according to the World Health Organization's 2022 Global tb *** 2021,10.6 million people were diagnosed with a new episode of tb and 1.6 million people died as a result.

关键词： tuberculosis death Mycobacterium