Identification of Orientia tsutsugamushi,spotted fever group and typhus group Rickettsia by duplex and nested PCR methods

作     者：M-C Luan D-Z Yu L Tang L-J Zhang 

作者机构：Department of RickettsiologyNational Institute of Communicable Disease Control and PreventionChina CDC Department of MicroorganismDalian Center for Disease Control and Prevention Department of MicroecologyDalian medical university Department of RickettsiologyNational Institute of Communicable Disease Control and PreventionChina.CDC 

出 版 物：《Asian Pacific Journal of Tropical Medicine》 (亚太热带医药杂志（英文版）)

年 卷 期：2008年第1卷第4期

页      面：1-8页

核心收录：

学科分类：1004[医学-公共卫生与预防医学(可授医学、理学学位)] 1002[医学-临床医学] 100401[医学-流行病与卫生统计学] 10[医学] 

基　　金：supported by National Natural Science Foundation of China(No. 30771854) China-U.S.Collaborative Program on Emerging and Re-emerging Infectious Diseases(No. 1U2GGH000018-01) 

主　　题：Rickettsia,GroEL gene Duplex and nested PCR 

摘      要：Objective:To identify members of genera of rickettsia and *** ***:Rapid and duplex and nested PCR methods have been established by designing primers based on the conserved regions of heat shock protein GroEL gene.345 mouse viscera samples including liver,spleen and kidney,96 Xenopsylla cheopis and 32 chiggers collected from Hongta areas of Yuxi city,Yunnan province were tested by the new PCR ***:The result of the study showed that the new PCR methods could identify most members of genera -Rickettsia and Orientia simultaneously with 100%specificity and its sensitivity could test one copy per *** results of detection prevalence of rickettsioses in mouse,flea and mites DNA samples showed that the total rickettsia infection rate in mouse was 34.78%(120/345).The total infection rates in ***,O.t Karp and *** of mouse samples were 28.12%(97/345),19.71%(68/345) and O. 29%(1/345) ***-infection rates in *** and 0.t Karp of mouse samples were 13.33%(46/ 345).O.t Karp type has been the main epidemic strain in these ***:We concluded that this PCR method could be used to detect multi-genera rickettsia *** evidences provided in this and previous studies strongly support that Hongta areas of Yuxi city are a natural focus for typhus and scrub typhus with the common occurrence of their confection.