Genetic Diversity and Drug Susceptibility of Mycobacterium tuberculosis Isolates in a Remote Mountain Area of China

作     者：ma ai jing wang sheng fen fan jia le zhao bing he guang xue zhao yan lin MA Ai Jing;WANG Sheng Fen;FAN Jia Le;ZHAO Bing;HE Guang Xue;ZHAO Yan Lin

作者机构：national institute for communicable disease control and preventionchinese center for disease control and preventionBeijing 102206China national center for tb control and preventionchinese center for disease control and preventionBeijing 102206China science and technology departmentchinese center for disease control and preventionBeijing 102206China 

出 版 物：《Biomedical and Environmental Sciences》 (生物医学与环境科学（英文版）)

年 卷 期：2018年第31卷第5期

页      面：351-362页

核心收录：

学科分类：1007[医学-药学(可授医学、理学学位)] 0830[工学-环境科学与工程（可授工学、理学、农学学位）] 100705[医学-微生物与生化药学] 1004[医学-公共卫生与预防医学(可授医学、理学学位)] 1001[医学-基础医学(可授医学、理学学位)] 100103[医学-病原生物学] 10[医学] 

基　　金：supported by the National Science and Technology Major Project 2014ZX100030002 The National Basic Research Program of China 2014CB744403 

主　　题：Mycobacterium tuberculosis Beijing strains Variable number tandem repeat (VNTR) Singlenucleotide polymorphism (SNP) 

摘      要：Objective We determined the genetic diversity of Mycobacterium tuberculosis（MTB） in a remote mountainous area of southwest China and evaluated the resolving ability of single nucleotide polymorphism（SNP） genotyping combined with variable number tandem repeat（VNTR） genotyping for Beijing family strains in association with drug resistance *** Three hundred thirty-one MTB strains were isolated from patients living in mountainous regions of southwest China,and 8-loci SNP,VNTR-15 genotyping assays,and drug susceptibility testing of 9 drugs were *** A total of 183 [55.29%（183/331）] strains were classified into the Beijing *** the 183 strains,111（60.66%） were defined as modern Beijing *** most predominant modern Beijing sub-lineage and ancient Beijing sub-lineage were Bmyc10 [39.34%（72/183）] and Bmyc25 [20.77%（38/183）],*** the isolates,19.64%（65/331） were resistant to at least 1 of the 9 anti-TB drugs and 17 [4.98%（17/331）] MTB isolates were multi-drug resistant tuberculosis（MDR-TB）.Two hundred sixty-one isolates showed a clustering rate of 14.18%（37/261） and a discriminatory index of *** Beijing lineage exhibited a significantly higher prevalence of MDR-TB,as well as resistance to isoniazid（INH）,rifampin（RIF）,and para-aminosalicylic acid（PAS） when analyzed independently（P = 0.005,P = 0.017,P = 0.014,and P = 0.006 respectively）.The Beijing lineage was not associated with genetic clustering or resistance to any *** addition,genetic clustering was not associated with drug *** MTB strains demonstrate high genetic diversity in remote mountainous areas of southwest *** strains,especially modern Beijing strains,are predominant in remote mountainous area of *** combination of 8-loci SNPs and VNTR-15 genotyping is a useful tool to study the molecular epidemiology of MTB strains in this area.