检索结果-南通市图书馆

Fine mapping and characterization of stripe rust resistance gene YrAYH in near-isogenic lines derived from a cross involving wheat landrace Anyuehong

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

The crop Journal 2024年第3期12卷 826-835页

作者： Li Long Jue Li Linyu Huang Huiling Jin Fangnian Guan Haipeng Zhang Sasa Zhao Hao Li Zhien Pu Wei Li Qiantao Jiang Yuming Wei Jian Ma Houyang Kang Shoufen Dai Pengfei Qi Qiang Xu Mei Deng Youliang Zheng Yunfeng Jiang Matthew James Moscou Guoyue Chen State Key Laboratory of crop Gene Exploration and Utilization in Southwest China Sichuan Agricultural UniversityChengdu 611130SichuanChina Triticeae Research Institute Sichuan Agricultural UniversityChengdu 611130SichuanChina College of Resources Sichuan Agricultural UniversityChengdu 611130SichuanChina The Sainsbury Laboratory Norwich Research ParkNorwich NR47UHEnglandUK United States Department of agriculture-agricultural Research Service Cereal Disease LaboratorySt.PaulMN 55108USA

Stripe rust,caused by Puccinia striiformis ***(Pst),is a devastating disease in wheat *** and characterizing new resistance genes/QTL is crucial for wheat breeding *** this study,we fine-mapped and characterized a stripe rust resistance gene,YRAYH,on chromosome arm 5BL in the Chinese wheat landrace Anyuehong(AYH).Evaluations of stripe rust response to prevalent Chinese Pst races in near-isogenic lines derived from a cross of Anyuehong and Taichung 29 showed that YrAYH conferred a high level of resistance at all growth *** mapping using a large segregating population of 9748 plants,narrowed the YRAYH locus to a 3.7 Mb interval on chromosome arm 5BL that included 61 annotated *** analysis of two NIL pairs identified 64 upregulated differentially expressed genes(DEGs)in the resistant NILs(NILs-R).Annotations indicated that many of these genes have roles in plant disease resistance *** a combined approach of fine-mapping and transcriptome sequencing,we identified a serine/threonine-protein kinase SRPK as a candidate gene underlying YrAYH.A unique 25 bp insertion was identified in the NILs-R compared to the NILs-S and previously published wheat *** InDel marker was developed and co-segregated with *** trait evaluation of the NILs suggested that YrAYH not only reduces the impact of stripe rust but was also associated with a gene that increases plant height and spike length.

关键词： Candidate gene analysis crop protection Puccinia striiformis Transcriptome analyses

EcR/USP-1-mediated ecdysteroid signaling regulates wolf spider(Pardosa pseudoannulata) development and reproduction

在线全文

学校读者我要写书评

暂无评论

Zoological Research 2023年第1期44卷 43-52页

作者： Zhi-Ming Yang Yang-Yang Yan Yong Wu Na Yu Ze-Wen Liu Key Laboratory of Integrated Management of crop Diseases and Pests(Ministry of Education) College of Plant ProtectionNanjing Agricultural UniversityNanjingJiangsu 210095China

Lycosidae females demonstrate meticulous maternal care of offspring by carrying egg sacs and juvenile spiderlings during the reproductive stage. Nuclear receptors(NRs), especially the ecdysone receptor(EcR) and ultraspiracle(USP), have attracted considerable attention in the regulation of arthropod development and reproduction due to their pivotal roles in ecdysteroid signaling cascades. In the present study, 23 NRs, including one EcR and two USPs, were identified in the genome of the predatory wolf spider Pardosa pseudoannulata. RNA interference(RNAi) targeting EcR and USP-1inhibited spiderling development and resulted in nonviable eggs in the egg sacs. EcR and USP-1responded to changes in ecdysteroid levels, and interference in ecdysteroid biosynthesis led to similar phenotypes as ds EcR and ds USP-1 *** findings suggest that EcR/USP-1-mediated ecdysteroid signaling regulates P. pseudoannulata development and reproduction. The *** females with suppressed ecdysteroid signaling proactively consumed their non-viable egg sacs, resulting in a 7.19 d shorter first reproductive cycle than the controls. Termination of the failed reproductive cycle enabled the spiders to produce a new egg sac more rapidly. This reproductive strategy may partially rescue the reduction in population growth due to non-viable eggs and compensate for the physiological expenditure of wasted maternal care, which would be beneficial for the conservation of *** populations and their natural control of insect pests.

关键词： Pardosa pseudoannulata Ecdysone receptor Ultraspiracle Development Reproduction

Molecular and morphological characterization of stunt nematodes of wheat, maize, and rice in the savannahs of northern Nigeria

在线全文

学校读者我要写书评

暂无评论

Journal of Integrative agriculture 2022年第2期21卷 586-595页

作者： Sulaiman ABDULSALAM PENG Huan LIU Shi-ming HUANG Wen-kun KONG Ling-an PENG De-liang State Key Laboratory for Biology of Plant Diseases and Insect Pests/Institute of Plant protection Chinese Academy of Agricultural SciencesBeijing 100193P.R.China Division of agricultural Colleges Department of Crop ProtectionAhmadu Bello UniversityZaria 810107Nigeria

Stunt nematodes(Tylenchorhynchus spp.)are obligate migratory root ecto-parasitic nematodes found in the fields of many cultivated *** nematodes,with phyto-sanitary potential,are frequently ignored or misdiagnosed as pests,and this may pose a threat to food *** accuracy of its identification based on a morphological approach has been challenged recently,due to the overlapping of the morphological and morphometric characters of the ***,the objective of this study is to identify and characterize stunt nematodes present in 54 fields cultivated with cereal crops(wheat,maize and rice)in the savannahs of northern Nigeria,using integrative taxonomy and molecular *** molecular and morphological studies identified and confirmed the presence of *** as the occurring specie in the savannahs of northern *** phylogenetic analysis was carried out using the internal transcribed spacer(ITS)and 28S genes of ribosomal DNA further confirmed the presence of *** first molecular characterization and sequences of the ITS and 28S rDNA gene for *** from Nigeria were provided by this ***,according to our literature search,this is the first report on *** in wheat,maize and rice in the savannahs of northern *** study to test the pathogenicity of the parasitic nematode species found in this survey is recommended for the prioritization and development of efficient management strategies.

关键词： plant-parasitic nematodes food security Tylenchorhynchus annulatus ITS phylogeny

The actions of neonicotinoid insecticides on nicotinic acetylcholine subunits Ldα1 and Ldα8 of Leptinotarsa decemlineata and assembled receptors

在线全文

学校读者我要写书评

暂无评论

Insect Science 2022年第5期29卷 1387-1400页

作者： Zhi-Min Wang Sha Li Cheng-Cheng Shi Lin-Jie Xie Kai-Yun Fu Wei-Hua Jiang College of Plant protection Nanjing Agricultural University/Key Laboratory of Integrated Management of Crop Disease and PestsMinistry of Education/Key Laboratory of Integrated Pest Management on Crops in East ChinaMinistry of AgricultureNanjing 210095China Institute of Plant protection Xinjiang Academy of Agricultural Sciences/Key Laboratory of Integrated Pest Management on Crop in Northwestern OasisMinistry Agriculture P.R.ChinaUrumqiChina

The insect nicotinic acetylcholine receptor(nAChR)is a pentameric channel protein and also a target for *** are few reported studies on the molecular interactions of Leptinotarsa decemlineata nAChRs with *** this study,we analyzed the response of acetylcholine and neonicotinoids(thiamethoxam[TMX],imidacloprid[IMI],and clothianidin[CLO])on hybrid receptors constructed by nAChRα1 andα8 subunits of ***(Ldα1 and Ldα8)co-expressed with ratβ2 subunit(rβ2)at different capped RNA(cRNA)ratios in Xenopus *** addition,we evaluated the expression changes of Ldα1 and Ldα8 after median lethal dose of TMX treatment for 72 h by quantitative polymerase chain reaction(qPCR).The resulting functional nAChRs Ldα1/rβ2 and Ldα1/Ldα8/rβ2 showed different pharmacological *** neonicotinoids tested showed lower agonist affinity on Ldα1/Ldα8/rβ2 compared to Ldα1/rβ2 at same ratios of subunit *** sensitivities of neonicotinoids tested for Ldα1/rβ2 and Ldα1/Ldα8/rβ2 at cRNA ratios of 5:1,1:1 and 5:5:1,1:1:1,respectively,were lower than those for nAChRs at ratios of 1:5 and 1:1:5,respectively,whereas the values of maximum response(Imax)*** Ldα1/Ldα8/rβ2,a reduction of Lda8 cRNA resulted in increased sensitivity to IMI and decreased sensitivity to *** expression of Ldα1 and Ldα8 significantly decreased in adults by 82.12%and 47.02%,respectively,while Ldα8 was significantly upregulated by 2.44 times in 4th instar larvae after exposure to *** infer that Ldα1 and Ldα8 together play an important role in the sensitivity of *** to neonicotinoids.

关键词： expression imidacloprid Leptinotarsa decemlineata nicotinic acetylcholine receptor thiamethoxam Xenopus oocyte

The Magnaporthe oryzae effector Avr-PikD suppresses rice immunity by inhibiting an LSD1-like transcriptional activator

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

The crop Journal 2024年第2期12卷 482-492页

作者： Jiayuan Guo Yiling Wu Jianqiang Huang Kaihui Yu Meilian Chen Yijuan Han Zhenhui Zhong Guodong Lu Yonghe Hong Zonghua Wang Xiaofeng Chen Fujian Universities Engineering Research Center of Marine Biology and Drugs Fuzhou Institute of OceanographyCollege of Geography and OceanographyMinjiang UniversityFuzhou 350108FujianChina Rice Research Institute Fujian Academy of Agricultural SciencesFuzhou 350018FujianChina Ministerial and Provincial Joint Innovation Center for Safety Production of Cross-Strait crops Minjiang UniversityFuzhou 350108FujianChina State Key Laboratory of Ecological Pest Control for Fujian and Taiwan crops College of Plant ProtectionFujian Agriculture and Forestry UniversityFuzhou 350002FujianChina

Avirulence effectors(Avrs),encoded by plant pathogens,can be recognized by plants harboring the corresponding resistance proteins,thereby initiating effector-triggered immunity(ETI).In susceptible plants,however,Avrs can function as effectors,facilitating infection via effector-triggered susceptibility(ETS).Mechanisms of Avr-mediated ETS remain largely *** we report that the Magnaporthe oryzae effector Avr-PikD enters rice cells via the canonical cytoplasmic secretion pathway and suppresses rice basal ***-PikD interacts with an LSD1-like transcriptional activator AKIP30 of rice,and AKIP30 is also a positive regulator of rice immunity,whereas Avr-PikD impedes its nuclear localization and suppresses its transcriptional *** summary,*** delivers Avr-PikD into rice cells to facilitate ETS by inhibiting AKIP30-mediated transcriptional regulation of immune response against ***.

关键词： Magnaporthe oryzae Avirulence effector Avr-PikD Effector-triggered susceptibility Rice immunity Transcriptional activator

agrobacterium tumefaciens-mediated transformation of embryogenic callus and CRISPR/Cas9-mediated genome editing in‘Feizixiao'litchi

在线全文

学校读者我要写书评

暂无评论

Horticultural Plant Journal 2023年第5期9卷 947-957页

作者： Shujun Wang Guo Wang Huanling Li Fang Li Jiabao Wang Environment and Plant protection Institute Chinese Academy of Tropical Agricultural SciencesHaikouHainan 571101China National Key Laboratory for Tropical crop Breeding HaikouHainan 571101China

Litchi(Litchi chinensis Sonn.)is a type of commercially prevalent subtropical and tropical *** litchi has a highly heterozygous genetic background and a long reproductive cycle,conventional breeding methods(such as hybridization)have limited ability to nurture new litchi ***,an efficient and stable agrobacterium tumefaciens-mediated genetic transformation of embryogenic callus was established in‘Feizixiao’*** materials were verified using polymerase chain reaction(PCR)analysis,β-glucuronidase(GUS)assay,and green fluorescent protein(GFP)*** implement the technology of the Clustered Regularly Interspaced Short Palindromic Repeats(CRISPR)/associated protein 9(CRISPR/Cas9)technology in‘Feizixiao’litchi and verify the validity of these transformation systems,the litchi polyphenol oxidase gene(LcPPO,JF926153)was knocked *** categories of mutations,covering base insertions,deletions,and substitutions,were found in transgenic materials via sequence *** transformation system achieved high feasibility and efficiency,and the system of CRISPR/Cas9 was successfully employed to edit genes in‘Feizixiao’*** work provides an essential foundation for investigating the functions of genes and accelerating litchi genetic improvement.

关键词： Litchi Litchi chinensis Sonn Genetic transformation Gene editing Polyphenol oxidase(PPO)

Susceptibility of northern corn rootworm Diabrotica barberi （Coleoptera： Chrysomelidae） to mCry3A and eCry3.1Ab Bacillus thuringiensis proteins

在线全文

学校读者我要写书评

暂无评论

Insect Science 2016年第6期23卷 913-917页

作者： Isaac O. Oyediran Phillip Matthews Narendra Palekar Wade French Jared Conville Tony Burd syngenta crop protection LLC Research Triangle Park NC 27709 USA USDA ARS North Central Agricultural Research Laboratory Brookings SD 57006 USA

The susceptibility of the northern corn rootworm Diabrotica barberi （Smith ＆ Lawrence） to mCry3A and eCry3.1Ab proteins derived from Bacillus thuringiensis （Bt） was determined using a diet bioassay. Northern corn rootworm neonates were exposed to different concentrations of mCry3A and eCry3.1Ab, incorporated into artificial diet. Larval mortality was evaluated after 7 d. The mCry3A and eCry3.1 Ab proteins were found to be toxic to the northern corn rootworm larvae. The LCs0 and LC99 values for mCry3A were 5.13 and 2482.31 μg/mL, respectively. For eCry3.1Ab, the LC50 and LC99 values were 0.49 and 213.01 μg/mL. Based on the estimated lethal concentrations, eCry3, lab protein was more efficacious to northern corn rootworm larvae than mCry3A. These lethal concentration values will be used as diagnostic doses for routine annual monitoring for change in susceptibility of field collected northern corn rootworm to mCry3A, and eCry3.1Ab toxins.

关键词： Bacillus thuringiensis Diabrotica barberi eCry3.1Ab mCry3A susceptibility

Understanding nutritive need in Harmonia axyridis larvae:Insights from nutritional geometry

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Insect Science 2022年第5期29卷 1433-1444页

作者： Shao-Lei Sun Nibijiang Abudisilimu Hao Yi Sali Li Tong-Xian Liu Xiangfeng Jing State Key Laboratory of crop Stress Biology for Arid Areas College of Plant ProtectionNorthwest A&F UniversityYanglingShaanxiChina Key Laboratory of Integrated Pest Management on the Loess Plateau of Ministry of agriculture College of Plant ProtectionNorthwest A&F UniversityYanglingShaanxiChina

The multicolored Asian lady beetle,Harmonia axyridis(Pallas)(Coleoptera:Coccinellidae),is an important natural enemy in agricultural *** spite of being a carnivore consuming protein-rich preys,the lady beetles often consume carbohydrate-rich food like nectar or ***,most studies on nutrition regulation of carnivores mainly focus on protein and lipid,two major macronutrients in *** this study,nutrition regulation of protein and carbohydrate has been investigated in the 4th instar larvae of *** using Geometric *** provided the insects two pairs of foods,one a protein-biased one and the second carbohydrate-biased,to determine the intake *** then confined them to nutritionally imbalanced foods to examine how they regulated food intake to achieve maximal *** larvae performed well on the 2 foods that containing the closest P:C ratios to the intake target,but,surprisingly,the lipid content was much lower than that in the choice *** lady beetles seemed to maintain the optimal lipid content by consuming carbohydrate-rich ***,consuming the carbohydrate-rich food was less metabolically expensive than the protein-rich ***,switching behavior between plant and animal foods actually reflects their nutritive *** findings extended our understanding of predator forage behavior and its influence on food web in ecosystems,and shed light on the role of agri-environment schemes in meeting the nutritional need of predators in field.

关键词： carbohydrate Harmonia axyridis nutritional geometry nutrition regulation protein

Reproduction does not impede the stopover departure to ensure a potent migration in Cnaphalocrocis medinalis moths

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Insect Science 2022年第6期29卷 1672-1684页

作者： Jiawen Guo Fan Yang Haiyan Zhang Peijiong Lin Baoping Zhai Zhongxian Lu Gao Hu Pengcheng Liu College of Plant protection Nanjing Agricultural UniversityNanjingChina State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of agro-Products Institute of Plant Protection and MicrobiologyZhejiang Academy of Agricultural SciencesHangzhouChina Institute of Vegetable Wuhan Academy of Agricultural ScienceWuhanChina Station of Plant protection and Plant Inspection Agricultural Technology Extension Center of Jiangyan DistrictTaizhouChina Wenzhou Station of Plant protection Soil and FertilizersWenzhouChina Key Laboratory of Integrated Management of crop Disease and Pests Ministry of EducationNanjing Agricultural UniversityNanjingChina Laboratory of Bio-interactions and crop Health Nanjing Agricultural UniversityNanjingChina

Despite the importance of reproduction in insects,its relation with multi-stop flight remains poorly understood in migratory *** clarify whether reproductive maturation commences during the multi-stop flight or after the completion of migration,we conducted physiological and behavioral assays in the rice leaf roller Cnaphalocrocis medinalis with laboratory-simulated conditions and field-captured *** found that the ovarian development was significantly promoted by tethered flight treatment for 1-2 nights when compared to the unflown group,while the flight muscle development was not *** was no significant difference in flight duration,flight distance and flight velocity between mated and virgin female moths,indicating that mated moths remained competent for the subsequent flights as did the *** an integrated field assay,we identified that over 60%of the female moths in the migrating populations cap-tured by high-altitude searchlights in the Immigration period of a season had completed the ovarian development and *** mature and mated moths collected in the rice field in the Emigration period were found capable of engaging in migratory take-off,as observed using an indoor monitoring ***,our findings point out that ***-inalis managed to complete reproductive maturation to a large extent during the multi-stop migratory flight without compromising the migration *** a cost-effective strategy ensures a successful migration for the *** findings advance our under-standing of the relationship between reproduction and migration,thus shedding light on the development of novel control measures for the outbreak of migratory insect pests.

关键词： mating multi-stop flight ovarian development pest control rice leaf roller