Fine mapping and characterization of stripe rust resistance gene YrAYH in near-isogenic lines derived from a cross involving wheat landrace Anyuehong

作     者：Li Long Jue Li Linyu Huang Huiling Jin Fangnian Guan Haipeng Zhang Sasa Zhao Hao Li Zhien Pu Wei Li Qiantao Jiang Yuming Wei Jian Ma Houyang Kang Shoufen Dai Pengfei Qi Qiang Xu Mei Deng Youliang Zheng Yunfeng Jiang Matthew James Moscou Guoyue Chen Li Long;Jue Li;Linyu Huang;Huiling Jin;Fangnian Guan;Haipeng Zhang;Sasa Zhao;Hao Li;Zhien Pu;Wei Li;Qiantao Jiang;Yuming Wei;Jian Ma;Houyang Kang;Shoufen Dai;Pengfei Qi;Qiang Xu;Mei Deng;Youliang Zheng;Yunfeng Jiang;Matthew James Moscou;Guoyue Chen

作者机构：State Key Laboratory of Crop Gene Exploration and Utilization in Southwest ChinaSichuan Agricultural UniversityChengdu 611130SichuanChina Triticeae Research InstituteSichuan Agricultural UniversityChengdu 611130SichuanChina College of ResourcesSichuan Agricultural UniversityChengdu 611130SichuanChina The Sainsbury LaboratoryNorwich Research ParkNorwich NR47UHEnglandUK United States Department of Agriculture-Agricultural Research ServiceCereal Disease LaboratorySt.PaulMN 55108USA 

出 版 物：《The Crop Journal》 (作物学报（英文版）)

年 卷 期：2024年第12卷第3期

页      面：826-835页

核心收录：

学科分类：0710[理学-生物学] 09[农学] 0901[农学-作物学] 

基　　金：supported by grants from the Major Program of National Agricultural Science and Technology of China(NK20220607) the National Natural Science Foundation of China(32272059 and 31971883) the Science and Technology Department of Sichuan Province(2021YFYZ0002,2022ZDZX0014,and 2023NSFSC1995) 

主　　题：Candidate gene analysis Crop protection Puccinia striiformis Transcriptome analyses 

摘      要：Stripe rust,caused by Puccinia striiformis ***(Pst),is a devastating disease in wheat *** and characterizing new resistance genes/QTL is crucial for wheat breeding *** this study,we fine-mapped and characterized a stripe rust resistance gene,YRAYH,on chromosome arm 5BL in the Chinese wheat landrace Anyuehong(AYH).Evaluations of stripe rust response to prevalent Chinese Pst races in near-isogenic lines derived from a cross of Anyuehong and Taichung 29 showed that YrAYH conferred a high level of resistance at all growth *** mapping using a large segregating population of 9748 plants,narrowed the YRAYH locus to a 3.7 Mb interval on chromosome arm 5BL that included 61 annotated *** analysis of two NIL pairs identified 64 upregulated differentially expressed genes(DEGs)in the resistant NILs(NILs-R).Annotations indicated that many of these genes have roles in plant disease resistance *** a combined approach of fine-mapping and transcriptome sequencing,we identified a serine/threonine-protein kinase SRPK as a candidate gene underlying YrAYH.A unique 25 bp insertion was identified in the NILs-R compared to the NILs-S and previously published wheat *** InDel marker was developed and co-segregated with *** trait evaluation of the NILs suggested that YrAYH not only reduces the impact of stripe rust but was also associated with a gene that increases plant height and spike length.