The actions of neonicotinoid insecticides on nicotinic acetylcholine subunits Ldα1 and Ldα8 of Leptinotarsa decemlineata and assembled receptors
作者机构：College of Plant ProtectionNanjing Agricultural University/Key Laboratory of Integrated Management of Crop Disease and PestsMinistry of Education/Key Laboratory of Integrated Pest Management on Crops in East ChinaMinistry of AgricultureNanjing 210095China Institute of Plant ProtectionXinjiang Academy of Agricultural Sciences/Key Laboratory of Integrated Pest Management on Crop in Northwestern OasisMinistry Agriculture P.R.ChinaUrumqiChina
出 版 物：《Insect Science》 (昆虫科学（英文版）)
年 卷 期：2022年第29卷第5期
页 面：1387-1400页
核心收录：
学科分类：090403[农学-农药学(可授农学、理学学位）] 09[农学] 0904[农学-植物保护]
基 金：funded by National Key Re-search and Development Project of China(No.2018YFD0200802)
主 题：expression imidacloprid Leptinotarsa decemlineata nicotinic acetylcholine receptor thiamethoxam Xenopus oocyte
摘 要：The insect nicotinic acetylcholine receptor(nAChR)is a pentameric channel protein and also a target for *** are few reported studies on the molecular interactions of Leptinotarsa decemlineata nAChRs with *** this study,we analyzed the response of acetylcholine and neonicotinoids(thiamethoxam[TMX],imidacloprid[IMI],and clothianidin[CLO])on hybrid receptors constructed by nAChRα1 andα8 subunits of ***(Ldα1 and Ldα8)co-expressed with ratβ2 subunit(rβ2)at different capped RNA(cRNA)ratios in Xenopus *** addition,we evaluated the expression changes of Ldα1 and Ldα8 after median lethal dose of TMX treatment for 72 h by quantitative polymerase chain reaction(qPCR).The resulting functional nAChRs Ldα1/rβ2 and Ldα1/Ldα8/rβ2 showed different pharmacological *** neonicotinoids tested showed lower agonist affinity on Ldα1/Ldα8/rβ2 compared to Ldα1/rβ2 at same ratios of subunit *** sensitivities of neonicotinoids tested for Ldα1/rβ2 and Ldα1/Ldα8/rβ2 at cRNA ratios of 5:1,1:1 and 5:5:1,1:1:1,respectively,were lower than those for nAChRs at ratios of 1:5 and 1:1:5,respectively,whereas the values of maximum response(Imax)*** Ldα1/Ldα8/rβ2,a reduction of Lda8 cRNA resulted in increased sensitivity to IMI and decreased sensitivity to *** expression of Ldα1 and Ldα8 significantly decreased in adults by 82.12%and 47.02%,respectively,while Ldα8 was significantly upregulated by 2.44 times in 4th instar larvae after exposure to *** infer that Ldα1 and Ldα8 together play an important role in the sensitivity of *** to neonicotinoids.
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