检索结果-南通市图书馆

Aging weakens Th17 cell pathogenicity and ameliorates experimental autoimmune uveitis in mice

Protein & cell 2022年第6期13卷 422-445页

作者： He Li Lei Zhu Rong Wang Lihui Xie Jie Ren Shuai Ma Weiqi Zhang Xiuxing Liu Zhaohao Huang Binyao Chen Zhaohuai Li Huyi Feng Guang-Hui Liu Si Wang Jing Qu Wenru Su state key Laboratory of Ophthalmology Zhongshan Ophthalmic CenterSun Yat-Sen UniversityGuangzhou 510060China state key Laboratory of Membrane biology Institute of ZoologyChinese Academy of SciencesBeijing 100101China state key Laboratory of stem cell and reproductive biology Institute of ZoologyChinese Academy of SciencesBeijing 100101China Advanced Innovation Center for Human Brain Protection National Clinical Research Center for Geriatric DisordersXuanwu Hospital Capital Medical UniversityBeijing 100053China CAS key Laboratory of Genomic and Precision Medicine Beijing Institute of GenomicsChinese Academy of SciencesBeijing 100101China Institute for stem cell and Regeneration Chinese Academy of SciencesBeijing 100101China Aging Translational Medicine Center International Center for Aging and CancerXuanwu HospitalCapital Medical UniversityBeijing 100053China China National Center for Bioinformation Beijing 100101China Beijing Institute for stem cell and Regenerative Medicine Beijing 100101China Chongqing Renji Hospital University of Chinese Academy of SciencesChongqing 400062China

Aging-induced changes in the immune system are associated with a higher incidence of infection and vaccination *** nodes,which filter the lymph to identify and fight infections,play a central role in this ***,careful characterization of the impact of aging on lymph nodes and associated autoimmune diseases is *** combined single-cell RNA sequencing(scRNA-seq)with flow cytometry to delineate the immune cell atlas of cervical draining lymph nodes(CDLNs)of both young and old mice with or without experimental autoimmune uveitis(EAU).We found extensive and complicated changes in the cellular constituents of CDLNs during *** confronted with autoimmune challenges,old mice developed milder EAU compared to young *** this EAU process,we highlighted that the pathogenicity of T helper 17 cells(Th17)was dampened,as shown by reduced GM-CSF secretion in old *** mitigated secretion of GMCSF contributed to alleviation of IL-23 secretion by antigen-presenting cells(APCs)and may,in turn,weaken APCs’effects on facilitating the pathogenicity of Th17 ***,our study further unveiled that aging downregulated GM-CSF secretion through reducing both the transcript and protein levels of IL-23R in Th17 cells from ***,aging altered immune cell responses,especially through toning down Th17 cells,counteracting EAU challenge in old mice.

关键词： aging experimental autoimmune uveitis Th17 cell APCs single-cell sequencing

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Mutations of MSH5 in nonobstructive azoospermia(NOA)and rescued via in vivo gene editing

引用

Signal Transduction and Targeted Therapy 2022年第2期7卷 349-351页

作者： Min Chen Chencheng Yao Yingying Qin Xiuhong Cui Peng Li Zhiyong Ji Limei Lin Haowei Wu Zhi Zhou Yaoting Gui Zheng Li Fei Gao Guangdong and Shenzhen key Laboratory of Male reproductive Medicine and Genetics Institute of UrologyPeking University Shenzhen HospitalShenzhen Peking University-The Hong Kong University of Science and Technology Medical CenterShenzhenGuangdong ProvinceP.R.China Department of Andrology the Center for Men’s HealthUrologic Medical CenterShanghai Key Laboratory of Reproductive MedicineShanghai General HospitalShanghai Jiao Tong University School of MedicineShanghaiP.R.China Center for reproductive Medicine of Shandong University National Research Center for Assisted Reproductive Technology and Reproductive GeneticsThe Key Laboratory for Reproductive Endocrinology of Ministry of EducationJinanP.R.China state key Laboratory of stem cell and reproductive biology Institute of ZoologyChinese Academy of SciencesBeijingP.R.China University of Chinese Academy of Sciences BeijingP.R.China Institute for stem cell and Regeneration Chinese Academy of SciencesBeijingChina School of Life Science and Technology ShanghaiTech UniversityShanghaiP.R.China

Dear Editor,Male infertility is a multifactorial heterogeneous pathological condition affecting∼7%of *** azoospermia(NOA)is one of the most severe male reproductive diseases and occurs in∼1%of men of reproductive ag... 详细信息

关键词： obstructive diseases vivo

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

An efficient protocol for studying human pluripotent stem cell-derived myotube senescence

引用

Biophysics Reports 2023年第5期9卷 232-240页

作者： Qian Zhao Ying Jing Shuai Ma Weiqi Zhang Jing Qu Si Wang Guang-Hui Liu Advanced Innovation Center for Human Brain Protection National Clinical Research Center for Geriatric Disorders Xuanwu Hospital Capital Medical University Aging Translational Medicine Center International Center for Aging and Cancer Beijing Municipal Geriatric Medical Research Center Xuanwu Hospital Capital Medical University state key Laboratory of Membrane biology Institute of Zoology Chinese Academy of Sciences Beijing Institute for stem cell and Regenerative Medicine Institute for stem cell and Regeneration CAS University of Chinese Academy of Sciences CAS key Laboratory of Genomic and Precision Medicine Beijing Institute of Genomics and China National Center for Bioinformation Chinese Academy of Sciences Sino-Danish College University of Chinese Academy of Sciences Sino-Danish Center for Education and Research state key Laboratory of stem cell and reproductive biology Institute of Zoology Chinese Academy of Sciences

Sarcopenia, an age-related skeletal muscle condition characterized by a progressive decline in muscle mass and function, is linked to increased vulnerability, a higher likelihood of falls, and higher mortality rates in older individuals. A comprehensive understanding of the intricate mechanisms driving skeletal muscle aging is of great significance in both scientific and clinical fields. Consequently, myotube models that facilitate studying regulatory mechanisms underlying skeletal muscle aging are important tools required to advance intervention strategies against skeletal muscle aging and associated ***, we provide a detailed protocol to generate human pluripotent stem cells-derived myotubes and describe their applications in aging studies, as well as a troubleshooting for potential problems. Overall,this protocol serves as a valuable methodological reference for exploring the role and mechanism of genes involved in skeletal muscle aging.

关键词： Human pluripotent stem cell Myotube Senescence Differentiation In vitro

来源：

同方期刊数据库评论

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Delivery of Cas9 Protein into Mouse Zygotes through a Series of Electroporation Dramatically Increases the Efficiency of Model Creation

引用

Journal of Genetics and Genomics 2016年第5期43卷 319-327页

作者： Wenbo Wang Peter M.Kutny Shannon L.Byers Charles J.Longstaff Michael J.DaCosta Changhong Pang Yingfan Zhang Robert A.Taft Frank W.Buaas Haoyi Wang The Jackson Laboratory Bar Harbor ME 04609 USA state key Laboratory of stem cell and reproductive biology Institute of Zoology Chinese Academy of Sciences Beijing 100101 China

Previously we established Zygote Electroporation of Nucleases（ZEN） technology as an efficient and high-throughput way to generate genetically modified mouse ***,there were significant variations of the targeting efficiency among different genomic loci using our previously published *** this study,we improved the ZEN technology by delivering Cas9 protein into mouse zygotes through a series of *** this approach,we were able to introduce precise nucleotide substitutions,large segment deletion and short segment insertion into targeted loci with high efficiency.

关键词： CRISPR-Cas9 Electroporation Mouse zygote

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

MeCP2 deficiency promotes cell reprogramming by stimulating IGF1/AKT/mTOR signaling and activating ribosomal protein-mediated ceil cycle gene translation

引用

Journal of Molecular cell biology 2018年第6期10卷 515-526页

作者： Wei Zhang Guihai Feng Libin Wang Fei Teng Liu Wang Wei Li Ying Zhang Qi Zhou state key Laboratory of stem cell and reproductive biology Institute of ZoologyChinese Academy of SciencesBeijing 100101China University of Chinese Academy of Sciences Beijing 100049China

The generation of induced pturipotent stem cells (iPSCs)offers a great opportunity in research and regenerative *** current poor efficiency and incomplete mechanistic understanding of the reprogramming process hamper the clinical application of iPSCs. MeCP2 connects histone modification and DNA methyiation,which are key changes of somatic cell ***,the rote of MeCP2 in celt reprogramming has not been *** this study,we found that MeCP2 deficiency enhanced reprogramming efficiency and stimulated cell proliferation through regulating cell cycle protein expression in the earLy stage of ***2 deficiency enhanced the expression of ribosomal protein genes,thereby enhancing reprogramming efficiency through promoting the translation of cell cycle *** the end,MeCP2 deficiency stimulated IGF1/AKT/mTOR signaling and activated ribosomal protein gene *** together,our data indicate that MeCP2 deficiency promoted cell reprogramming through stimulating IGF1/AKT/mTOR signaling and activating ribosomal protein-mediated cell cycle gene translation in the early stage of reprogramming.

关键词： MeCP2 cell reprogramming IGFI/AKT/mTOR signaling ribosomal protein cell cycle

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Treatment of multiple sclerosis by transplantation of neural stem cells derived from induced pluripotent stem cells

引用

Science China(Life Sciences) 2016年第9期59卷 950-957页

作者： Chao Zhang Jiani Cao Xiaoyan Li Haoyu Xu Weixu Wang Libin Wang Xiaoyang Zhao Wei Li Jianwei Jiao Baoyang Hu Qi Zhou Tongbiao Zhao state key Laboratory of stem cell and reproductive biology Institute of Zoology Chinese Academy of Sciences Beijing 100101 China Graduate University of Chinese Academy of Sciences Beijing 100049 China

Multiple sclerosis(MS) is an autoimmune disease of the central nervous system(CNS), with focal T lymphocytic infiltration and damage of myelin and axons. The underlying mechanism of pathogenesis remains unclear and there are currently no effective treatments. The development of neural stem cell(NSC) transplantation provides a promising strategy to treat neurodegenerative disease. However, the limited availability of NSCs prevents their application in neural disease therapy. In this study, we generated NSCs from induced pluripotent stem cells(iPSCs) and transplanted these cells into mice with experimental autoimmune encephalomyelitis(EAE), a model of MS. The results showed that transplantation of iPSC-derived NSCs dramatically reduced T cell infiltration and ameliorated white matter damage in the treated EAE mice. Correspondingly, the disease symptom score was greatly decreased, and motor ability was dramatically rescued in the iPSC-NSC-treated EAE mice, indicating the effectiveness of using iPSC-NSCs to treat MS. Our study provides pre-clinical evidence to support the feasibility of treating MS by transplantation of iPSC-derived NSCs.

关键词：神经干细胞多发性硬化移植治疗自身免疫性疾病中枢神经系统神经退行性疾病 NSCs 细胞浸润

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

cellular metabolism and homeostasis in pluripotency regulation

引用

Protein & cell 2020年第9期11卷 630-640页

作者： Kun Liu Jiani Cao Xingxing Shi Liang Wang Tongbiao Zhao state key Laboratory of stem cell and reproductive biology Institute of ZoologyChinese Academy of SciencesBeijing 100101China Institute for stem cell and Regeneration Chinese Academy of SciencesBeijing 100101China University of Chinese Academy of Sciences Beijing 100049China

Pluripotent stem cells(PSCs)can immortally self-renew in culture with a high proliferation rate,and they possess unique metabolic characteristics that facilitate pluripotency ***,we review recent progress in understanding the mechanisms that link cellular metabolism and homeostasis to pluripotency regulation,with particular emphasis on pathways involving amino acid metabolism,lipid metabolism,the ubiquitin-proteasome system and *** of amino acids and lipids is tightly coupled to epigenetic modification,organelle remodeling and cell signaling pathways for pluripotency *** harness enhanced proteasome and autophagy activity to meet the material and energy requirements for cellular *** regulatory events reflect a fine balance between the intrinsic cellular requirements and the extrinsic environment.A more complete understanding of this balance will pave new ways to manipulate PSC fate.

关键词： autophagy amino acid metabolism lipid metabolism pluripotent stem cell(PSC) ubiquitinproteasome system(UPS)

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Current advances in haploid stem cells

引用

Protein & cell 2020年第1期11卷 23-33页

作者： Tongtong Cui Zhikun Li Qi Zhou Wei Li state key Laboratory of stem cell and reproductive biology Institute of ZoologyChinese Academy of SciencesBeijing 100101China Institute for stem cell and Regeneration Chinese Academy of SciencesBeijing 100101China University of Chinese Academy of Sciences Beijing 100049China

Diploidy is the typical genomic mode in all *** stem cells are artificial cell lines experimentally derived in vitro in the form of different types of stem cells,which combine the characteristics of haploidy with a broad developmental potential and open the possibility to uncover biological mysteries at a genomic *** date,a multitude of haploid stem cell types from mouse,rat,monkey and humans have been derived,as more are in *** have been applied in high-throughput genetic screens and mammalian assisted ***,we review the generation,unique properties and broad applications of these remarkable cells.

关键词： haploidy parthenogenetic androgenetic stem cells diploidization functional genomics imprinting

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Domesticated cynomolgus monkey embryonic stem cells allow the generation of neonatal interspecies chimeric pigs

引用

Protein & cell 2020年第2期11卷 97-107页

作者： Rui Fu Dawei Yu Jilong Ren Chongyang Li Jing Wang Guihai Feng Xuepeng Wang Haifeng Wan Tianda Li Libin Wang Ying Zhang Tang Hai Wei Li Qi Zhou state key Laboratory of stem cell and reproductive biology Institute of ZoologyChinese Academy of SciencesBeijing 100101China Institute for stem cell and Regeneration Chinese Academy of SciencesBeijing 100101China University of Chinese Academy of Sciences Beijing 100049China

Blastocyst complementation by pluripotent stem cell(PSC)injection is believed to be the most promising method to generate xenogeneic ***,ethical issues prevent the study of human chimeras in the late embryonic stage of *** embryonic stem cells(ESCs),which have similar pluripotency to human ESCs,are a good model for studying interspecies chimerism and organ ***,whether primate ESCs can be used in xenogenous grafts remains *** this study,we evaluated the chimeric ability of cynomolgus monkey(Macaca fascicularis)ESCs(cmESCs)in pigs,which are excellent hosts because of their many similarities to *** report an optimized culture medium that enhanced the anti-apoptotic ability of cmESCs and improved the development of chimeric embryos,in which domesticated cmESCs(D-ESCs)injected into pig blastocysts differentiated into cells of all three germ *** addition,we obtained two neonatal interspecies chimeras,in which we observed tissue-specific D-ESC *** together,the results demonstrate the capability of D-ESCs to integrate and differentiate into functional cells in a porcine model,with a chimeric ratio of 0.001-0.0001 in different neonate *** believe this work will facilitate future developments in xenogeneic organogenesis,bringing us one step closer to producing tissue-specific functional cells and organs in a large animal model through interspecies blastocyst complementation.

关键词： embryonic stem cells,blastocyst complementation cynomolgus monkey pig interspecies chimera organ reconstruction

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Extended pluripotent stem cells facilitate mouse model generation

引用

Protein & cell 2019年第1期10卷 5-7页

作者： Guanghai Xiang Haoyi Wang state key Laboratory of stem cell and reproductive biology Institute of ZoologyChinese Academy of SciencesBeijing 100101China University of Chinese Academy of Sciences Beijing 100049China

Mouse embryonic stem(mES)cells,established in 1981(Evans and Kaufman,1981;Martin,1981),were derived from the inner cell mass(ICM)of blastocysts and can be expanded in vitro for many passages,maintaining normal karyotype and differentiation *** introduction into blastocysts,mES cells can differentiate into all three germ layers,contributing to all the somatic lineages and germline.

关键词： stem Martin cells

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：