Delivery of Cas9 Protein into Mouse Zygotes through a Series of Electroporation Dramatically Increases the Efficiency of Model Creation

作     者：Wenbo Wang Peter M.Kutny Shannon L.Byers Charles J.Longstaff Michael J.DaCosta Changhong Pang Yingfan Zhang Robert A.Taft Frank W.Buaas Haoyi Wang 

作者机构：The Jackson Laboratory Bar Harbor ME 04609 USA State Key Laboratory of Stem Cell and Reproductive Biology Institute of Zoology Chinese Academy of Sciences Beijing 100101 China 

出 版 物：《Journal of Genetics and Genomics》 (遗传学报（英文版）)

年 卷 期：2016年第43卷第5期

页      面：319-327页

核心收录：

学科分类：0710[理学-生物学] 1001[医学-基础医学(可授医学、理学学位)] 07[理学] 08[工学] 09[农学] 071009[理学-细胞生物学] 071007[理学-遗传学] 0901[农学-作物学] 0836[工学-生物工程] 090102[农学-作物遗传育种] 

基　　金：supported by the National Cancer Institute(grant No.P30CA034196) supported by the National Natural Science Foundation of China (No.31471215) Strategic Priority Research Program of the Chinese Academy of Sciences(No.XDA01010409) the National High Technology Research and Development Program("863" Program) of China(No.2015AA020307) 

主　　题：CRISPR Cas9 Electroporation Mouse zygote 

摘      要：Previously we established Zygote Electroporation of Nucleases（ZEN） technology as an efficient and high-throughput way to generate genetically modified mouse ***,there were significant variations of the targeting efficiency among different genomic loci using our previously published *** this study,we improved the ZEN technology by delivering Cas9 protein into mouse zygotes through a series of *** this approach,we were able to introduce precise nucleotide substitutions,large segment deletion and short segment insertion into targeted loci with high efficiency.