检索结果-南通市图书馆

A CRISPR/Cas12a-empowered surface plasmon resonance platform for rapid and specific diagnosis of the Omicron variant of SARS-CoV-2

引用

National Science Review 2022年第8期9卷 100-109页

作者： Zhi Chen Jingfeng Li Tianzhong Li Taojian Fan Changle Meng Chaozhou Li Jianlong Kang Luxiao Chai Yabin Hao Yuxuan Tang Omar A.Al-Hartomy Swelm Wageh Abdullah G.Al-Sehemi Zhiguang Luo Jiangtian Yu Yonghong Shao Defa Li Shuai Feng William J.Liu Yaqing He Xiaopeng Ma Zhongjian Xie Han Zhang Department of Physics Faculty of Science King Abdulaziz University Research Center for Advanced Materials Science (RCAMS) King Khalid University Department of Chemistry College of Science King Khalid University Zhongmin (Shenzhen) Intelligent Ecology Co.Ltd Key Laboratory of Optoelectronic Devices and Systems of Ministry of Education and Guangdong Province College of Physics and Optoelectronic Engineering Shenzhen University Department of Laboratory Medicine Shenzhen Children’s Hospital Optoelectronics Research Center School of Science Minzu University of China NHC Key Laboratory of Biosafety National Institute for Viral Disease Control and Prevention Chinese Center for Disease Control and Prevention Research Unit of Adaptive Evolution and Control of Emerging Viruses Chinese Academy of Medical Sciences Institute of Pathogenic Organism Shenzhen Center for Disease Control and Prevention Shenzhen Engineering Laboratory of Phosphorene and Optoelectronics Respiratory Department Shenzhen Children’s Hospital Institute of Pediatrics Shenzhen Children’s Hospital International Collaborative Laboratory of 2D Materials for Optoelectronics Science and Technology of Ministry of Education 3. Shenzhen Institute of Translational Medicine 4. Department of Otolaryngology Shenzhen Second People’s Hospital 5. the First Affiliated Hospital 6. Institute of Microscale Optoelectronics Shenzhen University 7. Shenzhen International Institute for Biomedical Research 8. Shenzhen Han’s Tech Limited Company 9. Shenzhen Metasensing Tech Limited Company

The outbreak of the COVID-19 pandemic was partially due to the challenge of identifying asymptomatic and presymptomatic carriers of the virus, and thus highlights a strong motivation for diagnostics with high sensitivity that can be rapidly deployed. On the other hand, several concerning SARS-Co V-2 variants,including Omicron, are required to be identified as soon as the samples are identified as ‘positive’.Unfortunately, a traditional PCR test does not allow their specific identification. Herein, for the first time,we have developed MOPCS(Methodologies of Photonic CRISPR Sensing), which combines an optical sensing technology-surface plasmon resonance(SPR) with the ‘gene scissors’ clustered regularly interspaced short palindromic repeat(CRISPR) technique to achieve both high sensitivity and specificity when it comes to measurement of viral variants. MOPCS is a low-cost, CRISPR/Cas12a-systemempowered SPR gene-detecting platform that can analyze viral RNA, without the need for amplification,within 38 min from sample input to results output, and achieve a limit of detection of 15 fM. MOPCS achieves a highly sensitive analysis of SARS-Co V-2, and mutations appear in variants B.1.617.2(Delta),B.1.1.529(Omicron) and BA.1(a subtype of Omicron). This platform was also used to analyze some recently collected patient samples from a local outbreak in China, identified by the Centers for Disease Control and Prevention. This innovative CRISPR-empowered SPR platform will further contribute to the fast, sensitive and accurate detection of target nucleic acid sequences with single-base mutations.

关键词： surface plasmon resonance CRISPR SARS-CoV-2 Omicron variant

来源：

同方期刊数据库评论

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

CRISPR-assisted transcription activation by phaseseparation proteins

引用

Protein & Cell 2023年第12期14卷 874-887页

作者： Jiaqi Liu Yuxi Chen Baoting Nong Xiao Luo Kaixin Cui Zhan Li Pengfei Zhang Wenqiong Tan Yue Yang Wenbin Ma Puping Liang Zhou Songyang State Key Laboratory of Biocontrol MOE Key Laboratory of Gene Function and Regulation and Guangzhou Key Laboratory of Healthy Aging ResearchSchool of Life SciencesSun Yat-senUniversityGuangzhou510275China Sun Yat-sen Memorial Hospital Sun Yat-sen UniversityGuangzhou 510275China Lumiere Therapeutics Suzhou215000China

The clustered regularly interspaced short palindromic repeats(CRISPR)-Cas9 system has been widely used for genome engineering and transcriptional regulation in many different organisms.Current CRISPR-activation(CRISPRa)platforms often require multiple components because of inefficient transcriptional activation.Here,we fused different phase-separation proteins to dCas9-VPR(dCas9-VP64-P65-RTA)and observed robust increases in transcriptional activation efficiency.Notably,human NUP98(nucleoporin 98)and FUS(fused in sarcoma)IDR domains were best at enhancing dCas9-VPR activity,with dCas9-VPR-FUS IDR(VPRF)outperforming the other CRISPRa systems tested in this study in both activation efficiency and system simplicity.dCas9-VPRF overcomes the target strand bias and widens gRNA designing windows without affecting the off-target effect of dCas9-VPR.These findings demonstrate the feasibility of using phase-separation proteins to assist in the regulation of gene expression and support the broad appeal of the dCas9-VPRF system in basic and clinical applications.

关键词： CRISPR transcriptional activation phase-separation proteins

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

CRISPR-Based Genome-Editing Tools for Huntington’s Disease Research and Therapy

引用

Neuroscience Bulletin 2022年第11期38卷 1397-1408页

作者： Yiyang Qin Shihua Li Xiao-Jiang Li Su Yang Guangdong Key Laboratory of Non-human Primate Research Guangdong-Hongkong-Macao Institute of CNS RegenerationJinan UniversityGuangzhou 510632China

Huntington’s disease(HD)is an autosomal dominantly-inherited neurodegenerative disease,which is caused by CAG trinucleotide expansion in exon 1 of the Huntingtin(HTT)gene.Although HD is a rare disease,its monogenic nature makes it an ideal model in which to understand pathogenic mechanisms and to develop therapeutic strategies for neurodegenerative diseases.Clustered regularly-interspaced short palindromic repeats(CRISPR)is the latest technology for genome editing.Being simple to use and highly efficient,CRISPR-based genome-editing tools are rapidly gaining popularity in biomedical research and opening up new avenues for disease treatment.Here,we review the development of CRISPR-based genome-editing tools and their applications in HD research to offer a translational perspective on advancing the genome-editing technology to HD treatment.

关键词： Huntington’s disease CRISPR Animal models

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Systematic identification of CRISPR off-target effects by CROss-seq

引用

Protein & Cell 2023年第4期14卷 299-303页

作者： Yan Li Shengyao Zhi Tong Wu Hong-Xuan Chen Rui Kang Dong-Zhao Mai Zhou Songyang Chuan He Puping Liang Guan-Zheng Luo IMOE Key Laboratory of Gene Function and Regulation Guangdong Province Key Laboratory of Pharmaceutical Functional GenesState Key Laboratory of BiocontrolSchool of Life SciencesSun Yat-sen UniversityGuangzhou 510275China Department of Chemistry University of ChicagoChicagoIL 60637USA Institute for Biophysical Dynamics Department of Biochemistry and Molecular BiologyHoward Hughes Medical InstituteUniversity of ChicagoChicagoIL 60637 USA

DearEditor,The CRISPR-mediated genome editing tools,including nucleases,base editors(ABE/CBE),transposases/recombinases,and prime editor(PE),have been extensively applied in basic and clinical researches,although the off-target effect remains a major concern(Anzalone et al.,2020).Recently,various methods have been developed to assess the specificity and accuracy of different tools(Zhang et al.,2021),yet each method is designed for limited editing systems,and none of them can simultaneously detect off-target sites in vivo and in vitro.A versatile method for profiling genome-wide off-target effects of various tools remains lacking.

关键词： CRISPR versatile editor

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

基于CRISPR的快速灵敏便捷分子检测

引用

生物工程学报 2023年第1期39卷 60-73页

作者：孙雯君黄行许王鑫杰上海科技大学生命科学与技术学院上海201210 中国农业科学院(深圳)农业基因组研究所、岭南现代农业科学与技术广东省实验室深圳分中心、农业农村部农业基因数据分析重点实验室广东深圳518120 广州生物岛实验室广东广州510030

快速灵敏检测技术对疾病防控必不可少。特别是新冠疫情暴发以来,人们深刻认识到快速灵敏检测技术的重要性。近年来,以CRISPR/Cas为代表的基因编辑技术带来了生物技术革命性的进步。CRISPR的核酸检测技术因其快速、准确、灵敏、经济等特... 详细信息

快速灵敏检测技术对疾病防控必不可少。特别是新冠疫情暴发以来,人们深刻认识到快速灵敏检测技术的重要性。近年来,以CRISPR/Cas为代表的基因编辑技术带来了生物技术革命性的进步。CRISPR的核酸检测技术因其快速、准确、灵敏、经济等特点,正在引发分子诊断革新,并已被成功应用于传染病、遗传病、肿瘤基因突变诊断,以及食品安全等领域。本文归纳了基于CRISPR的多种核酸检测体系及应用,并对未来CRISPR核酸检测发展趋势及结合人工智能的智能化检测进行了展望。

关键词：分子诊断核酸检测临场检测基因编辑 CRISPR CRISPR检测

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

The application of CRISPR/Cas technologies to Brassica crops:current progress and future perspectives

引用

aBIOTECH 2022年第2期3卷 146-161页

作者： Jun Li Xiaoxiao Yu Chao Zhang Na Li Jianjun Zhao State Key Laboratory of North China Crop Improvement and Regulation Cllge of Life SciencesHebei Agricultural UniversityBaoding 071001China Key Laboratory of Vegetable Germplasm Innovation and Utilization of Hebei ollaborative Innovation Center of Vegetable Industry in Hebei College of HorticultureHebei Agricultural UniversityBaoding 071001China

Brassica species are a global source of nutrients and edible vegetable oil for humans.However,all commercially important Brassica crops underwent a whole-genome triplication event,hindering the development of functional genomics and breeding programs.Fortunately,clustered regularly interspaced short palindromic repeat(CRISPR)/CRISPR-associated(Cas)technologies,by allowing multiplex and precise genome engineering,have become valuable genome-editing tools and opened up new avenues for biotechnology.Here,we review current progress in the use of CRISPR/Cas technologies with an emphasis on the latest breakthroughs in precise genome editing.We also summarize the application of CRISPR/Cas technologies to Brassica crops for trait improvements.Finally,we discuss the challenges and future directions of these technologies for comprehensive application in Brassica crops.Ongoing advancement in CRISPR/Cas technologies,in combination with other achievements,will play a significant role in the genetic improvement and molecular breeding of Brassica crops.

关键词： CRISPR Cas9 Cas12 Precise genome editing Brassica

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

基于CRISPR/Cas12b的乳粉中克罗诺杆菌属检测

引用

中国食品学报 2022年第6期22卷 267-275页

作者：张懿翔张清平李林显王亮杨捷琳刘洋上海市质量监督检验技术研究院/国家市场监管重点实验室(乳及乳制品检测与监控技术) 上海200233 吐露港生物科技有限公司上海200233 上海海关动植物与食品检验检疫技术中心上海200135

目的:开发一种基于CRISPR/Cas12b的克罗诺杆菌属分子检测方法。方法:以克罗诺杆菌菌属特异性基因序列为候选区域,针对区域设计扩增引物和向导RNA靶向序列,对其进行条件优化、特异性检测、反应体系优化,并用人工污染样品对体系的灵敏度... 详细信息

目的:开发一种基于CRISPR/Cas12b的克罗诺杆菌属分子检测方法。方法:以克罗诺杆菌菌属特异性基因序列为候选区域,针对区域设计扩增引物和向导RNA靶向序列,对其进行条件优化、特异性检测、反应体系优化,并用人工污染样品对体系的灵敏度、重复性进行验证。结果:该方法的特异性很好,方法的灵敏度达0.01 ng/μL,在人工污染样品中可检测到初始菌量CRISPR/Cas12b方法可用于乳粉中克罗诺杆菌属的快速检测。

关键词： CRISPR Cas12b 克罗诺杆菌属检测

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

基于分子信标策略的CRISPR/Cas12a荧光传感器用于循环肿瘤DNA的放大检测

引用

分析化学 2023年第2期51卷 184-193页

作者：张函笑代晓春周亚楠吕旭珍弓韬赵旭华于保锋山西医科大学基础医学院生物化学与分子生物学教研室

构建了一种以分子信标（Molecular beacon, MB）为信号探针的CRISPR/Cas12a生物传感器，用于循环肿瘤DNA(Circular tumor DNA, ctDNA)的快速放大检测。MB具有良好的稳定性，其颈部末端分别标记有荧光素（FAM）和四甲基罗丹明（TAMRA）... 详细信息

构建了一种以分子信标（Molecular beacon, MB）为信号探针的CRISPR/Cas12a生物传感器，用于循环肿瘤DNA(Circular tumor DNA, ctDNA)的快速放大检测。MB具有良好的稳定性，其颈部末端分别标记有荧光素（FAM）和四甲基罗丹明（TAMRA）两种荧光基团。ctDNA不存在时，CRISPR/Cas12a体系无活性，无法切割MB，因此MB两端的荧光基团由于形成发夹结构的颈部相互靠近而发生荧光共振能量转移（Fluorescence resonance energy transfer, FRET），显示出TAMRA的荧光。当ctDNA存在时，ctDNA特异性识别Cas12a/crRNA二元复合物并激活Cas12a的反式切割活性。由于单链DNA是Cas12a最敏感的底物，因此MB的环部单链首先被切割，继而引起颈部双链的解离而导致两种荧光团彼此远离，无法发生FRET，最终显示出FAM的荧光信号。对MB环部的碱基数目、MB浓度以及crRNA与Cas12a的浓度比例等实验条件进行了优化。在最优条件下，1.7～500 pmol/L范围内，ctDNA浓度与传感器在518 nm处的荧光强度呈线性关系，检出限为0.6 pmol/L(3σ)。将此传感器用于血清样品中ctDNA的检测，回收率在93%～110%之间。

关键词： CRISPR 生物传感器荧光共振能量转移循环肿瘤DNA

来源：

同方期刊数据库评论

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

The application of CRISPR-Cas in disease diagnosis and treatment

引用

中国科学:化学英文版 2023年第10期66卷 2734-2742页

作者： Zhiying Wang Yin Liu Fuling Zhou Yafen Wang Xiang Zhou School of Public Health Wuhan UniversityWuhan 430071China College of Chemistry and Molecular Sciences Wuhan UniversityWuhan 430072China Taikang Center for Life and Medical Sciences Wuhan UniversityWuhan 430071China Cross Research Institute of Zhongnan Hospital Wuhan UniversityWuhan 430071China Department of Hematology of Zhongnan Hospital Wuhan UniversityWuhan 430071China Department of Chemistry College of SciencesHuazhong Agricultural UniversityWuhan 430070China

Clustered regularly interspaced short palindromic repeat(CRISPR)has been gaining much attention in the modern medical field and has been widely used for the diagnosis and treatment of diseases in recent years.In this review,we will introduce the application of CRISPR in disease diagnosis and treatment,including its use in detecting pathogens,gene mutations,and genetic diseases,as well as its application in gene therapy for single-gene diseases,cancer,viral infectious diseases,and cardiovascular diseases.Additionally,we will discuss the potential future directions and challenges of CRISPR in the diagnosis and treatment of diseases,and provide a thorough overview of the ways in which CRISPR is used for diagnosing and treating diseases.

关键词： CRISPR application disease diagnosis disease treatment

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

CRISPR-Cas-based plant genome engineering goes viral

引用

Molecular Plant 2023年第4期16卷 660-661页

作者： Jose-Antonio Daros Fabio Pasin Fernando Merwaiss Instituto de Biologia Molecular y Celular de Plantas(CSiC-Universitat Politecnica deValencia) 46022ValenciaSpain

Biotechnological advances in the field of plant biology based on the bacterial and archaeal CRISPR-Cas systems promise a new generation of crop plants that are more productive and nutritive,resistant to pest and pathogens,and better adapted to environmental stresses.However,application of this technology in plants still encounters some burdens,especially related to the expression of the CRISPR-Cas reaction components in plant cells.

关键词： plant CRISPR adapted

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：