CRISPR-Mediated Engineering across the Central Dogma in Plant Biology for Basic Research and Crop Improvement

作     者：Dibyajyoti Pramanik Rahul Mahadev Shelake Mi Jung Kim Jae-Yean Kim Dibyajyoti Pramanik;Rahul Mahadev Shelake;Mi Jung Kim;Jae-Yean Kim

作者机构：Division of Applied Life Science(BK21 Four Program)Plant Molecular Biology and Biotechnology Research CenterGyeongsang National UniversityJinju 52828Korea These authors contributed equally to this article 

出 版 物：《Molecular Plant》 (分子植物（英文版）)

年 卷 期：2021年第14卷第1期

页      面：127-150页

核心收录：

学科分类：0710[理学-生物学] 071001[理学-植物学] 07[理学] 

基　　金：This work was supported by the National Research Foundation of Korea(grants NRF 2020M3A9I4038352,2020R1A6A1A03044344) by the Next-Generation BioGreen 21 Program(SSAC,grant PJ01322601) the Program for New Plant Breeding Techniques(NBT,grant PJ01478401),Rural Development Administration,Korea 

主　　题：central dogma CRISPR crop improvement gene regulation genome editing 

摘      要：The central dogma(CD)of molecular biology is the transfer of genetic information from DNA to RNA to *** CD processes governing genetic flow include the cell cycle,DNA replication,chromosome packaging,epigenetic changes,transcription,posttranscriptional alterations,translation,and posttranslational *** CD processes are tightly regulated in plants to maintain genetic integrity throughout the life cycle and to pass genetic materials to next *** of various CD processes involved in gene regulation will accelerate crop improvement to feed the growing world *** technology enables programmable editing of CD processes to alter DNA,RNA,or protein,which would have been impossible in the ***,an overview of recent advancements in CRISPR tool development and CRISPR-based CD modulations that expedite basic and applied plant research is ***,CRISPR applications in major thriving areas of research,such as gene discovery(allele mining and cryptic gene activation),introgression(de novo domestication and haploid induction),and application of desired traits beneficial to farmers or consumers(biotic/abiotic stress-resilient crops,plant cell factories,and delayed senescence),are ***,the global regulatory policies,challenges,and prospects for CRISPR-mediated crop improvement are discussed.