A CRISPR/Cas12a-empowered surface plasmon resonance platform for rapid and specific diagnosis of the Omicron variant of SARS-CoV-2
A CRISPR/Cas12a-empowered surface plasmon resonance platform for rapid and specific diagnosis of the Omicron variant of SARS-CoV-2作者机构：Department of Physics Faculty of Science King Abdulaziz University Research Center for Advanced Materials Science (RCAMS) King Khalid University Department of Chemistry College of Science King Khalid University Zhongmin (Shenzhen) Intelligent Ecology Co.Ltd Key Laboratory of Optoelectronic Devices and Systems of Ministry of Education and Guangdong Province College of Physics and Optoelectronic Engineering Shenzhen University Department of Laboratory Medicine Shenzhen Children’s Hospital Optoelectronics Research Center School of Science Minzu University of China NHC Key Laboratory of Biosafety National Institute for Viral Disease Control and Prevention Chinese Center for Disease Control and Prevention Research Unit of Adaptive Evolution and Control of Emerging Viruses Chinese Academy of Medical Sciences Institute of Pathogenic Organism Shenzhen Center for Disease Control and Prevention Shenzhen Engineering Laboratory of Phosphorene and Optoelectronics Respiratory Department Shenzhen Children’s Hospital Institute of Pediatrics Shenzhen Children’s Hospital International Collaborative Laboratory of 2D Materials for Optoelectronics Science and Technology of Ministry of Education 3. Shenzhen Institute of Translational Medicine 4. Department of Otolaryngology Shenzhen Second People’s Hospital 5. the First Affiliated Hospital 6. Institute of Microscale Optoelectronics Shenzhen University 7. Shenzhen International Institute for Biomedical Research 8. Shenzhen Han’s Tech Limited Company 9. Shenzhen Metasensing Tech Limited Company
出 版 物：《National Science Review》 (国家科学评论(英文版))
年 卷 期：2022年第9卷第8期
页 面：100-109页
核心收录：
学科分类：0710[理学-生物学] 100208[医学-临床检验诊断学] 1002[医学-临床医学] 1001[医学-基础医学(可授医学、理学学位)] 10[医学]
基 金：supported by the Shenzhen Key Medical Discipline Construction Fund (SZXK032) the Guangdong Scientific and Technological Project (2019B1515120043,2020A151501612, 2021A1515220109 and 2022B1515020093) the Science and Technology Innovation Commission of Shenzhen (KCXFZ20201221173413038) the Longhua District Science and Innovation Commission Project Grants of Shenzhen (JCYJ201904) the Innovation Team Project of the Department of Education of Guangdong Province(2018KCXTD026) supported by the Deanship of Scientific Research (DSR) at King Abdulaziz University,Jeddah (KEP-MSc-70-130-42) the funding from King Khalid University through the Research Center for Advanced Materials Science (RCAMS)(RCAMS/KKU/006/21) the Shenzhen Science and Technology Innovation Commission Key Project (JSGG20200225152648408) the Shenzhen Science and Technology Plan Project (JCYJ20200109105608771)
主 题：surface plasmon resonance CRISPR SARS-CoV-2 Omicron variant
摘 要：The outbreak of the COVID-19 pandemic was partially due to the challenge of identifying asymptomatic and presymptomatic carriers of the virus, and thus highlights a strong motivation for diagnostics with high sensitivity that can be rapidly deployed. On the other hand, several concerning SARS-Co V-2 variants,including Omicron, are required to be identified as soon as the samples are identified as ‘positive’.Unfortunately, a traditional PCR test does not allow their specific identification. Herein, for the first time,we have developed MOPCS(Methodologies of Photonic CRISPR Sensing), which combines an optical sensing technology-surface plasmon resonance(SPR) with the ‘gene scissors’ clustered regularly interspaced short palindromic repeat(CRISPR) technique to achieve both high sensitivity and specificity when it comes to measurement of viral variants. MOPCS is a low-cost, CRISPR/Cas12a-systemempowered SPR gene-detecting platform that can analyze viral RNA, without the need for amplification,within 38 min from sample input to results output, and achieve a limit of detection of 15 fM. MOPCS achieves a highly sensitive analysis of SARS-Co V-2, and mutations appear in variants B.1.617.2(Delta),B.1.1.529(Omicron) and BA.1(a subtype of Omicron). This platform was also used to analyze some recently collected patient samples from a local outbreak in China, identified by the Centers for Disease Control and Prevention. This innovative CRISPR-empowered SPR platform will further contribute to the fast, sensitive and accurate detection of target nucleic acid sequences with single-base mutations.
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