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Efficient production of chemicals from microorganism by metabolic engineering and synthetic biology

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Chinese Journal of Chemical Engineering 2021年第2期34卷 14-28页

作者： Yang Zhang Jing Yu Yilu Wu Mingda Li Yuxuan Zhao Haowen Zhu Changjing Chen Meng Wang Biqiang Chen Tianwei Tan National Energy r&d center for Biorefinery Beijing University of Chemical TechnologyBeijing 100029China

The use of traditional chemical catalysis to produce chemicals has a series of drawbacks,such as high dependence on fossil resources,high energy consumption,and environmental pollution.With the development of synthetic biology and metabolic engineering,the use of renewable biomass raw materials for chemicals synthesis by constructing efficient microbial cell factories is a green way to replace traditional chemical catalysis and traditional microbial fermentation.This review mainly summarizes several types of bulk chemicals and high value-added chemicals using metabolic engineering and synthetic biology strategies to achieve efficient microbial production.In addition,this review also summarizes several strategies for effectively regulating microbial cell metabolism.These strategies can achieve the coupling balance of material and energy by regulating intracellular material metabolism or energy metabolism,and promote the efficient production of target chemicals by microorganisms.

关键词： Chemicals Synthetic biology Metabolic regulation Microbial cell factory

Modulation of the Anisotropic Electronic Properties in reS_(2) via Ferroelectric Film

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CCS Chemistry 2019年第3期1卷 268-277页

作者： renyan Wang Fengya Zhou Liang Lv Shasha Zhou Yiwei Yu Fuwei Zhuge Huiqiao Li Lin Gan Tianyou Zhai State Key Laboratory of Material Processing and die&Mould Technology School of Materials Science and EngineeringHuazhong University of Science and TechnologyWuhan 430074China Shenzhen R&D Center Huazhong University of Science and TechnologyShenzhen 518000China

Anisotropic response in two-dimensional materials has emerged with attractive potential applications in angle-sensitive areas.However,the challenge with current studies on anisotropic response is due to the main use of the structural materials of pristine to examine the physical properties in different direc-tions,which cause restriction of the anisotropic ratio within a narrow range,thereby,limiting their practical applications.

关键词： anisotropic reS_(2) PVdF polarization ferroelectric

Numerical and Experimental Evaluation of Shine-Through Loss and Beam Heating due to Neutral Beam Injection on EAST

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Chinese Physics Letters 2021年第5期38卷 46-50页

作者：王进芳李颖颖吴斌陈玉庆李军许永建陈龙溪郝保龙周登黄娟丁斯晔杨振侯雅巍刘晓娟项农 Institute of Plasma Physics Chinese Academy of SciencesHefei 230031China ENN Fusion Technology r&d center Langfang 065001China School of Information Engineering Shandong Youth University of Political ScienceJi’nan 250103China Advanced Energy research center Shenzhen UniversityShenzhen 518060China CAS Key Laboratory of Geospace Environment and department of Plasma Physics and Fusion Engineering University of Science and Technology of ChinaHefei 230026China KTX Laboratory and department of Plasma Physics and Fusion Engineering University of Science and Technology of ChinaHefei 230026China

This research applies experimental measurements and NUBEAM,ONETWO and TrANSP modules to investigate the shine-through(ST)loss ratio and beam heating percentage of neutral beam injection on EAST.Measurements and simulations confirm that the ST loss ratio increases linearly with beam energy,and decreases exponentially with plasma density.Moreover,using the multi-step fitting method,we present analytical quantitative expressions of ST loss ratio and beam heating percentage,which are valuable for the high parameter long-pulse experiments of EAST.

关键词： Numerical and Experimental Evaluation of Shine-Through Loss and Beam Heating due to Neutral Beam Injection on EAST

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China Communications 2016年第S1期13卷 72-86页

作者： ZHANG Guo ZHANG Jianhui WANG Binqiang ZHANG Zhen National digital Switching System Engineering & Technological r&d center

Towards line speed and accurateness on-line content popularity monitoring on Content Centric Networking(CCN) routers, we propose a three-stage scheme based on Bloom filters and hash tables for differentiated traffic. At the first stage, we decide whether to deliver the content to the next stage depending on traffic types. The second stage consisting of Standard Bloom filters(SBF) and Counting Bloom filters(CBF) identifies the popular content. Meanwhile, a scalable sliding time window based monitoring scheme for different traffic types is proposed to implement frequent and real-time updates by the change of popularities. Hash tables according with sliding window are used to record the popularity at the third stage. Simulation results reveal that this method reaches a 40 Gbps processing speed at lower error probability with less memory, and it is more sensitive to the change of popularity. Additionally, the architecture which can be implemented in CCN router is flexible and scalable.

关键词： CCN line speed traffic type bloom filters hash tables popularity monitoring

Annular flexible thermoelectric devices with integrated-module architecture

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npj Flexible Electronics 2020年第1期4卷 1-7页

作者： dawei Qu Xuan Huang Xin Li Hanfu Wang Guangming Chen College of Materials Science and Engineering Shenzhen UniversityShenzhen 518055P.R.China Beijing Key Laboratory of Clothing Materials r&d and Assessments Beijing Engineering Research Center of Textile NanofiberSchool of Materials Science&EngineeringBeijing Institute of Fashion TechnologyBeijing 100029P.R.China National center for Nanoscience and Technology of China Beijing 100190P.R.China

Organic and composite thermoelectric(TE)materials have witnessed explosive developments in recent years.design strategy of their flexible devices is vital to achieve high performance and suit various application environments.Here,we propose a design strategy of annular flexible TE devices with integrated-module architecture,where the independent modules made up of alternatively connected p-n couples are connected in series,and then rounded head-to-tail into annular configuration.The achieved devices can not only save plenty of space owing to their highly integrated structure design,but also be directly mounted on cylindrical objects(like pipes)to suit versatile applications.More importantly,the annular TE devices display excellent performances,superior to most previous work and the traditional serial single-layer film structure.For example,the annular device with eight modules consisting of three p-n couples reveals an output power of 12.37μW at a temperature gradient of 18 K,much higher than that of the corresponding single-layer film structure(1.74μW).The integration process is simple and easy to scale up.This architecture design strategy will greatly speed up the TE applications and benefit the research of organic and composite TE materials.

关键词： composite film mounted

近红外二区荧光可视化超声联合吲哚菁绿微泡开放血脑屏障增强脑胶质瘤光热治疗

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Science Bulletin 2022年第22期67卷 2316-2326页

作者：梁思敏胡德红黎国锋高笃阳李飞郑海荣潘敏盛宗海 department of Ultrasonography Shenzhen Hospital(Futian)of Guangzhou University of Chinese MedicineShenzhen 518034China Paul C.Lauterbur research center for Biomedical Imaging CAS Key Laboratory of Health InformaticsShenzhen Key Laboratory of Ultrasound Imaging and TherapyInstitute of Biomedical and Health EngineeringShenzhen Institute of Advanced TechnologyChinese Academy of SciencesShenzhen 518055China School of Biomedical Engineering Guangdong Medical UniversityDongguan 523808China

聚焦超声开放血脑屏障是增强胶质母细胞瘤治疗的关键,然而,缺乏一种高时空分辨率的体内成像方法用于原位同步监测血脑屏障开放过程.本文报道了使用吲哚菁绿掺杂的微泡(MBs-ICG)可视化聚焦超声开放血脑屏障和增强对抗胶质母细胞瘤的光热... 详细信息

聚焦超声开放血脑屏障是增强胶质母细胞瘤治疗的关键,然而,缺乏一种高时空分辨率的体内成像方法用于原位同步监测血脑屏障开放过程.本文报道了使用吲哚菁绿掺杂的微泡(MBs-ICG)可视化聚焦超声开放血脑屏障和增强对抗胶质母细胞瘤的光热治疗方法.结果显示, MBs-ICG具有明亮的近红外二区荧光,超声造影成像和超声响应特性.利用其互补的成像特性, MBs-ICG可实现脑血管的高分辨率(~168.9μm)近红外二区荧光成像、大穿透深度(~7 mm)超声造影成像.通过近红外二区荧光成像, MBs-ICG联合聚焦超声可原位同步可视化血脑屏障开放,信噪比为6.2±1.2.此外,在聚焦超声辐照下, MBs-ICG转化为脂质ICG纳米颗粒,并在10 min内迅速进入肿瘤组织,成功用于增强原位胶质母细胞瘤小鼠的光热治疗.多功能MBs-ICG为监测血脑屏障开放和增强胶质母细胞瘤治疗提供了一种新的范式.

关键词：吲哚菁绿光热治疗胶质母细胞瘤血脑屏障聚焦超声荧光成像近红外脑胶质瘤

Self-assembly multifunctional dNA tetrahedron for efficient elimination of antibiotic-resistant bacteria

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Aggregate 2024年第1期5卷 204-215页

作者： Tiantian Wu Yu Fu Shuang Guo Yanqiang Shi Yuxin Zhang Zhijin Fan Bin Yang Baoquan ding Yuhui Liao Molecular diagnosis and Treatment center for Infectious diseases Dermatology HospitalSouthern Medical UniversityGuangzhouChina CAS Key Laboratory of Nanosystem and Hierarchical Fabrication CAS Center for Excellence in NanoscienceNational Center for Nanoscience and TechnologyBeijingChina Longgang district Central Hospital of shenzhen ShenzhenChina

Antibiotic resistance is a major challenge in the clinical treatment of bacterial infectious diseases.Herein,we constructed a multifunctional dNA nanoplatform as a versatile carrier for bacteria-specific delivery of clinical antibiotic ciprofloxacin(CIP)and classic nanoantibiotic silver nanoparticles(AgNP).In our rational design,CIP was efficiently loaded in the self-assembly double-bundle dNA tetrahedron through intercalation with dNA duplex,and single-strand dNA-modified AgNP was embedded in the cavity of the dNA tetrahedron through hybridization.With the site-specific assembly of targeting aptamer in the well-defined dNA tetrahedron,the bacteria-specific dual-antibiotic delivery system exhibited excellent combined bactericidal properties.With enhanced antibiotic accumulation through breaking the out membrane of bacteria,the antibiotic delivery system effectively inhibited biofilm formation and promoted the healing of infected wounds in vivo.This dNAbased antibiotic delivery system provides a promising strategy for the treatment of antibiotic-resistant infections.

关键词： antibiotic resistance anti-infection therapy biofilm dNA nanotechnology targeted delivery

构建重定向的NF-κB/OIP5表达程序以增强化疗对膀胱癌的治疗效果

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Science Bulletin 2023年第24期68卷 3207-3224,M0006页

作者：郑彬彬牛立慢徐海波杨育宾陈延萍王晨光陈巍黄卫人 department of Urology Shenzhen Institute of Translational MedicineShenzhen Second People’s HospitalThe First Affiliated Hospital of Shenzhen UniversityInternational Cancer Center of Shenzhen UniversityShenzhen 518039China shenzhen Institute of Synthetic Biology Shenzhen Institutes of Advanced TechnologyChinese Academy of SciencesShenzhen 518055China Guangdong Key Laboratory of Systems Biology and Synthetic Biology for Urogenital Tumors Shenzhen 518035China

NF-κB是重要的转录调控因子,对下游肿瘤耐药基因起关键调控作用.基于CrISPr基因编辑技术,可在膀胱癌中构建人工合成的基因表达程序,以重编程肿瘤药物应答过程.本研究发现Opa相互作用蛋白5(OIP5)在NF-κB激活后上调,并引起膀胱癌对长春... 详细信息

NF-κB是重要的转录调控因子,对下游肿瘤耐药基因起关键调控作用.基于CrISPr基因编辑技术,可在膀胱癌中构建人工合成的基因表达程序,以重编程肿瘤药物应答过程.本研究发现Opa相互作用蛋白5(OIP5)在NF-κB激活后上调,并引起膀胱癌对长春新碱耐药.通过整合连接靶向OIP5的引导rNA与NF-κB的适配体rNA,成功获得兼具编码靶向位点和调控功能的模块化rNA.此模块化rNA可识别并与长春新碱活化的NF-κB相结合,促使NF-κB从上调逆转为下调OIP5表达,并阻断多条NF-κB介导的耐药通路,从而高效阻止肿瘤耐药的发生.本研究还开发了一种纳米递药系统共递送模块化rNA和长春新碱,体内外实验表明两者协同增强药物的抗肿瘤疗效.通过基于纳米递药系统的联合治疗策略,我们证实了CrISPr技术在膀胱癌中构建人工合成基因表达程序的有效性,可重编程肿瘤的药物应答过程,达到增强膀胱癌化疗敏感性的目的.

关键词： drug resistance Gene circuit rNA engineering NF-jB OIP5 Vincristine

Genomic alterations and molecular subtypes of gastric cancers in Asians

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Chinese Journal of Cancer 2016年第8期35卷 403-409页

作者： Xiang S.Ye Chunping Yu Amit Aggarwal Christoph reinhard Lilly(China) r&d center Lilly research Laboratories Lilly Corporate CenterEli Lilly and Company

Gastric cancer(GC) is a highly heterogenic disease,and it is the second leading cause of cancer death in the world.Common chemotherapies are not very effective for GC,which often presents as an advanced or metastatic disease at diagnosis.Treatment options are limited,and the prognosis for advanced GCs is poor.The landscape of genomic alterations in GCs has recently been characterized by several international cancer genome programs,including studies that focused exclusively on GCs in Asians.These studies identified major recurrent driver mutations and provided new insights into the mutational heterogeneity and genetic profiles of GCs.An analysis of gene expression data by the Asian Cancer research Group(ACrG) further uncovered four distinct molecular subtypes with well-defined clinical features and their intersections with actionable genetic alterations to which targeted therapeutic agents are either already available or under clinical development.In this article,we review the ACrG GC project.We also discuss the implications of the genetic and molecular findings from various GC genomic studies with respect to developing more precise diagnoses and treatment approaches for GCs.

关键词： Gastric cancer Cancer genome Molecular subtyping Heterogeneity Oncogenic drivers Targeted therapy