近红外二区荧光可视化超声联合吲哚菁绿微泡开放血脑屏障增强脑胶质瘤光热治疗

作     者：梁思敏 胡德红 黎国锋 高笃阳 李飞 郑海荣 潘敏 盛宗海 Simin Liang;Dehong Hu;Guofeng Li;Duyang Gao;Fei Li;Hairong Zheng;Min Pan;Zonghai Sheng

作者机构：Department of UltrasonographyShenzhen Hospital(Futian)of Guangzhou University of Chinese MedicineShenzhen 518034China Paul C.Lauterbur Research Center for Biomedical ImagingCAS Key Laboratory of Health InformaticsShenzhen Key Laboratory of Ultrasound Imaging and TherapyInstitute of Biomedical and Health EngineeringShenzhen Institute of Advanced TechnologyChinese Academy of SciencesShenzhen 518055China School of Biomedical EngineeringGuangdong Medical UniversityDongguan 523808China 

出 版 物：《Science Bulletin》 (科学通报（英文版）)

年 卷 期：2022年第67卷第22期

页      面：2316-2326页

核心收录：

学科分类：0710[理学-生物学] 1002[医学-临床医学] 07[理学] 1010[医学-医学技术（可授医学、理学学位）] 070302[理学-分析化学] 100214[医学-肿瘤学] 0703[理学-化学] 10[医学] 0702[理学-物理学] 1009[医学-特种医学] 

基　　金：supported by the National Natural Science Foundation of China (92159304, 82171958, 81901812, 81971638, 91859117, 82027803, and 81927807) CAS Key Laboratory of Health Informatics (2011DP173015) the Science and Technology Key Project of Shenzhen(JCYJ20190812163614809, JCYJ20200109114612308, JCYJ2021032 4120011030, JCYJ20190809105207439, JCYJ20220531091408019, and JCYJ20200109114825064) Guangdong Basic and Applied Basic Research Fund (2020A1515110011, 2020A1515010395, and 2022A1515010384) Key Laboratory for Magnetic Resonance and Multimodality Imaging of Guangdong Province (2020B1212060051) the Key Technology and Equipment R&D Program of Major Science and Technology Infrastructure of Shenzhen (202100102, 202100104) Discipline Construction Project of Guangdong Medical University (4SG21017G) 

主　　题：吲哚菁绿 光热治疗 胶质母细胞瘤 血脑屏障 聚焦超声 荧光成像 近红外 脑胶质瘤 

摘      要：聚焦超声开放血脑屏障是增强胶质母细胞瘤治疗的关键,然而,缺乏一种高时空分辨率的体内成像方法用于原位同步监测血脑屏障开放过程.本文报道了使用吲哚菁绿掺杂的微泡(MBs-ICG)可视化聚焦超声开放血脑屏障和增强对抗胶质母细胞瘤的光热治疗方法.结果显示, MBs-ICG具有明亮的近红外二区荧光,超声造影成像和超声响应特性.利用其互补的成像特性, MBs-ICG可实现脑血管的高分辨率(~168.9μm)近红外二区荧光成像、大穿透深度(~7 mm)超声造影成像.通过近红外二区荧光成像, MBs-ICG联合聚焦超声可原位同步可视化血脑屏障开放,信噪比为6.2±1.2.此外,在聚焦超声辐照下, MBs-ICG转化为脂质ICG纳米颗粒,并在10 min内迅速进入肿瘤组织,成功用于增强原位胶质母细胞瘤小鼠的光热治疗.多功能MBs-ICG为监测血脑屏障开放和增强胶质母细胞瘤治疗提供了一种新的范式.