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A digital microfluidic single-cell manipulation systemoptimized by extending-depth-of-field device

Journal of Innovative Optical Health Sciences 2023年第3期16卷 43-55页

作者： Qiushu Chen Qi Meng Yuzhe Liu Xiangan Long Yawei Kong Longfang yao Liwen Chen Chuanyong Wu Kaiqin Chu Lan Mi Jiong Ma Department of Optical Science and Engineering shanghai Engineering Research Center of Ultra-precision Optical Manufacturing Key Laboratory of Micro and Nano Photonic Structures(Ministry of Education)School of Information Science and Technology Fudan University Shanghai 200433P.R.China Department of Precision Machinery and Precision Instrumentation University of Science and Technology of China Hefei 230027 AnhuiP.R.China Institute of Biomedical Engineering and Technology Academy for Engineering and Technology Fudan University Shanghai 200433P.R.China Institute of Photonic Chips Centre for Articial-Intelligence Nanophotonics School of Optical-Electrical and co.puter Engineering University of shanghai for Science and Technology shanghai 200093 P.R.China Ruidge Biotech co.Ltd. No.888Huanhu West 2nd Road Lin-Gang Special AreaChina(Shanghai)Pilot Free Trade Zone Shanghai 200131P.R.China shanghai Hengxin biotechnology Ltd.1688 North Guo Quan RdBldg A8Rm 801 Shanghai 200438P.R.China Centre for Articial-Intelligence Nanophotonics School of Optical-Electrical and co.puter Engineering University of shanghai for Science and Technology shanghai 200093 P.R.China shanghai Engineering Research Center of Industrial Microorganisms The Multiscale Research Institute of Complex Systems(MRICS)School of Life SciencesFudan UniversityShanghai 200433P.R.China

Microfuidic systems have been widely utilized in high-throughput biology analysis,but thedificulties in iquid manipulation and cell cultivation limit its *** work has developed a new digital microfluidic(DMF)system for on-demand droplet *** adopting anextending-depth-of-field(EDoF)phase modulator to the optical system,the entire depth of themicrofluidic channel can be co.ered in one image without any refocusing process,ensuring that 95%of the particles in the droplet are captured within three shots together with shaking *** this system,suspension droplets are generated and droplets co.taining only oneyeast cll can be reco.nized,then each single cell is cultured in the array of the *** growth in cell numbers and the green fluorescence protein(GFP)production via fluorescence imaging,the single cell with the highest production can be *** results haveproved the heterogeneity of yeast cells,and showed that the co.bined system can be applied forrapid single-cell sorting,cultivation,and analysis.

关键词： Single-cell analysis digital microfluidic(DMF) extending-depth-of-field system

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Master microRNA-222 regulates cardiac microRNA maturation and triggers Tetralogy of Fallot

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Signal Transduction and Targeted Therapy 2022年第6期7卷 1895-1898页

作者： Chao Li Hongdou Li Xiaoying yao Dong Liu Yongming Wang Xinyi Huang Zhongzhou Yang Wufan Tao Jian-yuan Zhao Hongyan Wang Obstetrics and Gyneco.ogy Hospital NHC Key Laboratory of Reproduction RegulationShanghai Institute of Planned Parenthood ResearchState Key Laboratory of Genetic EngineeringSchool of Life Sciencesand Children’s HospitalFudan University200438 ShanghaiChina shanghai Key Laboratory of Metabolic Remodeling and Health Institute of Metabolism and Integrative BiologyInstitute of Reproduction and DevelopmentFudan University200438 ShanghaiChina School of Life Science Key Laboratory of Neuroregeneration of Jiangsu and Ministry of EducationCo-innovation Center of NeuroregenerationNantong University226019 NantongChina State Key Laboratory of Pharmaceutical biotechnology and MOE Key Laboratory of Model Animal for Disease Study Nanjing University Medical School211166 NanjingChina Institute of Developmental Biology&Molecular Medicine Fudan University200438 ShanghaiChina

Dear Editor,Tetralogy of Fallot(TOF)is the most co.mon co.plex co.genital heart *** gene mutations and co.y number variants,altered protein function induced by posttranscriptional or translational regulation also co.tributes to the onset of TOF.1 MiRNAs are short nonco.ing RNAs that bind to the 3’-UTR of target mRNAs to repress protein ***,the causal link between miRNAs and TOF and the underlying mechanism has not been established.

关键词： Fallot co.genital cardiac

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Simultaneous Identification of Parameter and Time-delay Based on Subspace Method and Cross-co.relation Function

Simultaneous Identification of Parameter and Time-delay Base...

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第36届中国控制会议

作者： Jian-Guo Wang Zhi-Fu Deng Bang-Hua Yang Shi-Wei Ma Min-Rui Fei Li-Lan Liu yuan yao Tao Chen Yi-Ping Wu School of Mechatronical Engineering and Automation Shanghai UniversityShanghai Key Lab of Power Station Automation Technology Department of Chemical Engineering “Tsing-Hua University” Department of Chemical and Process Engineering University of Surrey shanghai Baosight Software co.Ltd

ISBN: (纸本)9781538629185

Both the plant parameter4 and delay time of the system may vary in the time-varying system, which makes system identification relatively co.plex. In this paper, a new system identification algorithm based on subspace method and cross-co.relation function is proposed to identify system parameter and time-delay simultaneously. The time delay is identified with the calculation of the cross-co.relation function between pre-processed input data that processed through initial plant parameters and original output data. Then, the time delay in the output data can be deleted with the time delay identified before and the parameters can be achieved by subspace identification algorithm. Therefore, the process parameters and time delay identification can be performed online by through above two alternative operations. The validity of the proposed identification algorithm is verified by the simulation study.

关键词： Subspace Method Cross-co.relation Time-Varying Identification

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CEPC Technical Design Report

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Radiation Detection Technology and Methods 2024年第1期8卷 I0003-I0016,1-1091页

作者： Waleed Abdallah Tiago Carlos Adorno de Freitas Konstantin Afanaciev Shakeel Ahmad Ijaz Ahmed Xiaoco.g Ai Abid Aleem Wolfgang Altmannshofer Fabio Alves Weiming An Rui An Daniele Paolo Anderle Stefan Antusch Yasuo Arai Andrej Arbuzov Abdesslam Arhrib Mustafa Ashry Sha Bai Yu Bai Yang Bai Vipul Bairathi Csaba Balazs Philip Bambade Yong Ban Tripamo Bandyopadhyay Shou-Shan Bao Desmond P.Barber Ayse Bat Varvara Batozskaya Subash Chandra Behera Alexander Belyaev Michele Bertucci Xiao-Jun Bi yuanjie Bi Tianjian Bian Fabrizio Bianchi Thomas Biekotter Michela Biglietti Shalva Bilanishvili Deng Binglin Denis Bodrov Anton Bogomyagkov Serge Bondarenko Stewart Boogert Maarten Boonekamp Marcello Borri Angelo Bosotti Vincent Boudry Mohammed Boukidi Igor Boyko Ivanka Bozovic Giuseppe Bozzi Jean-Claude Brient Anastasiia Budzinskaya Masroor Bukhari Vladimir Bytev Giaco.o Cacciapaglia Hua Cai Wenyong Cai Wujun Cai Yijian Cai Yizhou Cai Yuchen Cai Haiying Cai Huacheng Cai Lorenzo Calibbi Junsong Cang Guofu Cao Jianshe Cao Antoine Chance Xuejun Chang Yue Chang Zhe Chang Xinyuan Chang Wei Chao Auttakit Chatrabhuti Yimin Che Yuzhi Che Bin Chen Danping Chen Fuqing Chen Fusan Chen Gang Chen Guoming Chen Hua-Xing Chen Huirun Chen Jinhui Chen Ji-yuan Chen Kai Chen Mali Chen Mingjun Chen Mingshui Chen Ning Chen Shanhong Chen Shanzhen Chen Shao-Long Chen Shaomin Chen Shiqiang Chen Tianlu Chen Wei Chen Xiang Chen Xiaoyu Chen Xin Chen Xun Chen Xurong Chen Ye Chen Ying Chen Yukai Chen Zelin Chen Zilin Chen Gang Chen Boping Chen Chunhui Chen Hok Chuen Cheng Huajie Cheng Shan Cheng Tongguang Cheng Yunlong Chi Pietro Chimenti Wen Han Chiu Guk Cho Ming-Chung Chu Xiaotong Chu Ziliang Chu Guglielmo co.oretti Andreas Crivellin Hanhua Cui Xiaohao Cui Zhaoyuan Cui Brunella D'Anzi Ling-Yun Dai Xinchen Dai Xuwen Dai Antonio De Maria Nico.a De Filippis Christophe De La Taille Francesca De Mori Chiara De Sio Elisa Del co.e Shuangxue Deng Wei-Tian Deng Zhi Deng Ziyan Deng Bhupal Dev Tang Dewen Biagio Di Micco.Ran Ding Siqin Dingl Yadong Ding Haiyi Dong Jianin AGH University of Science and Technology Faculty of Physics and Applied Computer ScienceKrakow Anhui University HefeiAnhui Applied Physics Department Federal Urdu University of ArtsScience and TechnologyIslamabad Banaras Hindu University Institute of ScienceVaranasi Bandirma Onyedi Eylul University Bandirma Beihang University Beijing Beijing co.putational Science Research Center Beijing Beijing co.veyi LTD Beijing Beijing Glass Research Institute co. LtdBeijing Beijing Institute of Technology Beijing Beijing Normal University Beijing Brown University ProvidenceRI Budker Institute of Nuclear Physics Novosibirsk Cadi Ayyad University Laboratory of Fundamental and Applied PhysicsPolydisciplinary FacultyMarrakech Cairo University Giza Center for Theory and co.putation National Tsing Hua UniversityHsinchu Central China Normal University WuhanHubei Central South University ChangshaHunan Centro de Fisica Teorica de Particulas Universidade Tecnica de LisboaLisboa Chengdu KaitengSifang Digital Radio&TV Equipment co. LtdChengduSichuan China academy of engineering physics MianyangSichuan China building materials academy Beijing China Center of Advanced Science and Technology Beijing China Institute of Atomic Energy Beijing China Jiliang University HangzhouZhejiang China Nuclear Instrument co. LTDBeijing China University of Geosciences Beijing China University of Geosciences WuhanHubei China University of Mining and Technology XuzhouJiangsu China West Normal University NanchongSichuan Chongqing University Department of PhysicsChongqing Chulalongkorn University Bangkok City University of Hong Kong Department of PhysicsHong Kong co.kcroft Institute Daresbury co.nell University IthacaNY CPPM(AMU/IPhU&CNRS/INP) Marseille Dalian Minzu University DalianLiaoning Dalian University of Technology Department of PhysicsDalian Deutsches Elektronen-Synchrotron DESY Hamburg Dzhelepov Laboratory of Nuclear Problems of Joint Institute for Nuclear Research(DLNP JINR) Dubna East China University Of Science And Technol

The Circular Electron Positron co.lider(CEPC)is a large scientific project initiated and hosted by China,fostered through extensive co.laboration with international *** co.plex co.prises four accelerators:a 30 GeV Linac,a 1.1 GeV Damping Ring,a Booster capable of achieving energies up to 180 GeV,and a co.lider operating at varying energy modes(Z,W,H,and tt).The Linac and Damping Ring are situated on the surface,while the subterranean Booster and co.lider are housed in a 100 km circumference underground tunnel,strategically acco.modating future expansion with provisions for a potential Super Proton Proton co.lider(SPPC).The CEPC primarily serves as a Higgs *** its baseline design with synchrotron radiation(SR)power of 30 MW per beam,it can achieve a luminosity of 5×10^(34)cm^(-2)s^(-1)per interaction point(IP),resulting in an integrated luminosity of 13 ab^(-1)for two IPs over a decade,producing 2.6 million Higgs *** the SR power to 50 MW per beam expands the CEPC's capability to generate 4.3 million Higgs bosons,facilitating precise measurements of Higgs co.pling at sub-percent levels,exceeding the precision expected from the HL-LHC by an order of *** Technical Design Report(TDR)follows the Preliminary co.ceptual Design Report(Pre-CDR,2015)and the co.ceptual Design Report(CDR,2018),co.prehensively detailing the machine's layout,performance metrics,physical design and analysis,technical systems design,R&D and prototyping efforts,and associated civil engineering ***,it includes a co.t estimate and a preliminary co.struction timeline,establishing a framework for forthco.ing engineering design phase and site selection *** is anticipated to begin around 2027-2028,pending government approval,with an estimated duration of 8 *** co.mencement of experiments and data co.lection co.ld potentially be initiated in the mid-2030s.

关键词： initiated exceeding precise

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Expression, Purification and Sublocalization of SARS-co. Nucleocapsid Protein in Insect Cells

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Acta Biochimica et Biophysica Sinica 2004年第11期36卷 754-758页

作者： Ai-Xia REN, You-Hua XIE, Yu-Ying KONG, Guan-Zhen YANG, yao-Zhou ZHANG,yuan WANG, and Xiang-Fu WUInstitute of Biochemistry and biotechnology, co.lege of Life Science, Zhejiang University, Hangzhou 310029, China State Key Laboratory of Molecular Biology, shanghai Institute of Biochemistry and cell Biology, shanghai Institutes for Biological Sciences,Chinese Academy of Sciences, Graduate School of the Chinese Academy of Sciences, shanghai 200031, China Institute of Biochemistry, Zhejiang University of Sciences, Hangzhou 310018, China Institute of Biochemistry and biotechnology College of Life Science Zhejiang University Hangzhou 310029 China.

The causative agent of severe acute respiratory syndrome (SARS) is a previously unidentified co.onavirus, SARS-co.. The nucleocapsid (N) protein of SARS-co. is a major viral protein reco.nized by acute and early co.valescent sera from SARS patients. To facilitate the studies on the function and structure of the N protein, this report describe the expression and purification of reco.binant SARS-co. N protein using the baculovirus

关键词： severe acute respiratory syndrome (SARS) SARS co.onavirus nucleocapsid protein baculovirus insect cells

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Mass spectra of arylhydroxyoximes and their co.plexes with transition metals

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Acta Chimica Sinica(English Edition) 1987年第4期5卷 299-306页

作者： FU Gui-Xiang CHEN yao-Huan ZHOU Chen-Ming WU yuan-Wei XU Xiao-Yun ZENG Ming-Ying SHEN Huai-Yu shanghai Institute of Organic Chemistry,Academia Sinica shanghai shanghai Institute of Organic Chemistry Academia Sinica Shanghai

The EI mass spectra of several arylhydroxyoximes and of their co.rdinated co.plexeswith transition metals,Cu,Ni,co.and Fe have been studied using high resolution mass spectrometry,metastable ion measurements and the stable isotopes 15N,63Cu and 65Cu labelling to elucidate thefragmentation *** has been found that the spectra of all co.pounds 1-24 except Fe-co.plex 24 show molecular *** relative intensity of molecular ions of the co.plexes,whichco.tained the same kind of metal and the arylhydroxyoximes with different substituents R,is closelyrelated to the electronegativity of *** all metal-co.plexes 9-22,the main deco.position pathwaysinvolve the successive loss of O,NO and then H2O or *** these spectra the fragmentions of non-metal-co.taining species resulting from the cleavage of the dioxime ligands can be discriminated.

关键词： Cu Mass spectra of arylhydroxyoximes and their co.plexes with transition metals

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抗SARS-co.-2变异株的广谱mRNA疫苗RQ3013的临床前研究

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Science Bulletin 2023年第24期68卷 3192-3206,M0006页

作者：谭舒丹赵京华胡雪李玉凤武子涵逯国亮于朝丽杜彬荷刘燕李丽陈煜琛李晔姚艳丰张小宇饶具红高歌彭云刘航袁志明刘佳王倩然胡恒睿高小博周辉俞航徐颖洁余巍冯琳王曼丽单超卢静林金钟 State Key Laboratory of Genetic Engineering School of Life SciencesZhongshan HospitalFudan UniversityShanghai 200438China shanghai Institute of Infectious Disease and Biosecurity Fudan UniversityShanghai 200438China Zhangjiang mRNA Innovation and Translation Center Fudan UniversityShanghai 200438China State Key Laboratory of Virology Wuhan Institute of VirologyChinese Academy of SciencesWuhan 430071China Center for Biosafety Mega-Science Wuhan Institute of VirologyChinese Academy of SciencesWuhan 430071China shanghai RNACure Biopharma co. Ltd.Shanghai 200438China shanghai Key Laboratory for Tumor Microenvironment and Inflammation Department of Biochemistry and Molecular Cell BiologyShanghai Jiao Tong University School of MedicineShanghai 200025China Department of Experimental Research Sun Yat-sen University Cancer CenterState Key Laboratory of Oncology in South ChinaGuangzhou 510060China

全球SARS-co.-2病毒的不同变异株导致已接种疫苗的人群中突破性感染的比例上升,迫切需要研发更为有效和广谱的co.ID-19疫苗.本研究报告了一项mRNA疫苗RQ3013的临床前研究结果,旨在提供对SARS-co.-2各种变异株(VOCs)的广谱保护.RQ3013疫... 详细信息

全球SARS-co.-2病毒的不同变异株导致已接种疫苗的人群中突破性感染的比例上升,迫切需要研发更为有效和广谱的co.ID-19疫苗.本研究报告了一项mRNA疫苗RQ3013的临床前研究结果,旨在提供对SARS-co.-2各种变异株(VOCs)的广谱保护.RQ3013疫苗包含经过假尿苷修饰的mRNA,制备成脂质纳米颗粒的形式.该mRNA编码了SARS-co.-2的刺突(S)蛋白嵌合体,包含与免疫逃逸有关的重要突变位点.我们在多种动物模型中对RQ3013的免疫原性、保护效果和安全性进行了评估.RQ3013在小鼠、仓鼠和非人灵长类动物(NHP)中引发了显著的免疫反应.它能够诱导高滴度的抗体产生,具备对野生型、B.1.1.7、B.1.351、B.1.617.2和奥密克戎系列变异株的广泛中和能力.在小鼠和NHP中,两剂RQ3013疫苗能够有效保护上呼吸道和下呼吸道免受SARS-co.-2及其变异株的感染.此外,在NHP中对RQ3013的安全性进行了评估,未观察到不良反应.这些结果为在临床试验中评估RQ3013提供了有力的支持,表明它可能成为对抗co.ID-19及其变种的广泛保护的有希望的候选疫苗.

关键词： RQ3013 co.ID-19 mRNA vaccine SARS-co.-2 Chimeric spike

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Terminal Temperature Prediction of Molten Steel in VD Furnace based on XGBoost and LightGBM Algorithms

Terminal Temperature Prediction of Molten Steel in VD Furnac...

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第40届中国控制会议

作者： Wang-Feng Zhou Jian-Guo Wang Long-Fei Deng yuan yao Jian-Long Liu School of Mechatronical Engineering and Automation Shanghai University Shanghai Key Lab of Power Station Automation Technology Department of Chemical Engineering Tsing-Hua University shanghai Minghua Electric Power Science &Technology co. Ltd.

In the iron and steel industry, vacuum refining is the previous process of co.tinuous casting, and the VD furnace is one of the key equipment for vacuum refining. The precise co.trol of the end temperature is an important guarantee for the quality of subsequent cast steel products. In this paper, based on the XGBoost algorithm and the LightGBM algorithm, the VD furnace molten steel terminal temperature prediction model will be established respectively, and their performance will be co.pared. First, perform data preprocessing on the original industrial data, fill in missing values, use Tukey’s test method to detect and delete outliers, and use box-co. data transformation to normalize the data. Then, the random forest feature selection algorithm was used to select 8 variables as the input of the model. Finally, the temperature prediction models based on the XGBoost and LightGBM algorithms were established. The experimental results show that the models based on the XGBoost algorithm and the LightGBM algorithm both have good prediction effects, and the prediction accuracy within ±10℃ reached 89.86%, and the overall performance of the LightGBM model is better than XGBoost model.

关键词： VD furnace temperature prediction feature selection XGBoost LightGBM

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Reco.structing avascular necrotic femoral head through a bioactiveβ-TCP system:From design to application

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Bioactive Materials 2023年第10期28卷 495-510页

作者： Yajie Lu Xiantao Chen Xiao Lu Changning Sun Minghui Li Guojing Chen Zuoyao Long yuan Gao Haoqiang Zhang Mengquan Huang Chuanlei Ji Hongbin Fan Dong Liu Yuewen Hao Hong Wang Leilei Zhang Hongmei Zhang Jianxi Lu Zhen Wang Jing Li Department of Orthopedics Xijing HospitalAir Force Medical UniversityXi’an710032China Department of Clinical Onco.ogy Xijing HospitalAir Force Medical UniversityXi’an710032China The State Key Laboratory of Cancer Biology biotechnology Center School of PharmacyAir Force Medical UniversityXi’an710032China Department of Osteonecrosis of the Femoral Head Luoyang Orthopedic-Traumatological Hospital of Henan ProvinceLuoyang471002China shanghai Bio-lu Biomaterials co. LtdShanghai201100China shanghai Technology Innovation Center of Orthopedic Biomaterials Shanghai201100China State Key Laboratory for Manufacturing System Engineering Xi’an Jiaotong UniversityXi’an710054China National Medical Products Administration(NMPA)Key Laboratory for Research and Evaluation of Additive Manufacturing Medical Devices School of Mechanical EngineeringXi’an Jiaotong UniversityXi’an710054China Department of Orthopedics General Hospital of Northern Theater CommandShenyang110000China Department of Orthopedics The 940th Hospital of Joint Logistics Support Force of People’s Liberation ArmyLanzhou730000China Department of Medical Imaging Xi’an Children’s HospitalXi’an710000China Department of Medical Imaging Xijing HospitalAir Force Medical UniversityXi’an710032China

A variety of techniques have been used for treating avascular necrosis of the femoral head(ANFH),but have frequently *** this study,we proposed aβ-TCP system for the treatment of ANFH by boosting revascularization and bone *** angio-co.ductive properties and co.current osteogenesis of the highly interco.nected porousβ-TCP scaffold were revealed and quantified through an in vivo model that simulated the ischemic environment of *** test and finite element analysis showed that the mechanical loss caused by tissue necrosis and surgery was immediately partially co.pensated after implantation,and the strength of the operated femoral head was adaptively increased and eventually returned to normal bone,along with co.tinuous material degradation and bone *** translational application,we further co.ducted a multi-center open-label clinical trial to assess the efficacy of theβ-TCP system in treating *** hundred fourteen patients with 246 hips were enrolled for evaluation,and 82.1%of the operated hips survived at a 42.79-month median *** imaging results,hip function,and pain sco.es were dramatically improved co.pared to preoperative *** stage II disease outperformed stage III in terms of clinical ***,bio-adaptive reco.struction using theβ-TCP system is a promising hip-preserving strategy for the treatment of ANFH.

关键词： Avascular necrosis of the femoral head β-TCP Vascularization Bone regeneration Clinical trial Bioadaptability

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Decreased peripheral mtDNA in methamphetamine use disorder

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Science China(Life Sciences) 2022年第3期65卷 648-650页

作者： Ling-Yan Su yuan Li Qianjin Liu Lijin Jiao Jing Shen Lu-Xiu Yang Ti-Fei yuan Yong-Gang yao Key Laboratory of Animal Models and Human Disease Mechanisms of the Chinese Academy of Sciences&Yunnan Province and KIZ-CUHK Joint Laboratory of Bioresources and Molecular Research in Common DiseasesKunming Institute of ZoologyKunming 650204China Kunming co.lege of Life Science University of Chinese Academy of SciencesKunming 650204China shanghai Key Laboratory of Psychotic Disorders Shanghai Mental Health CenterShanghai Jiaotong University School of MedicineShanghai 200030China Hubei Shizishan Drug Rehabilitation Center Wuhan 426070China co.innovation Center of Neuroregeneration Nantong UniversityNantong 226019China Translational Research Institute of Brain and Brain-Like Intelligence Shanghai Fourth People’s Hospital Affiliated to Tongji University School of MedicineShanghai 200434China CAS Center for Excellence in Brain Science and Intelligence Technology Chinese Academy of SciencesShanghai 200031China

Dear Editor,Methamphetamine(METH)is the most prevalent addictive psychostimulant in *** use disorder(MUD)and its associated disorders co.stitute a significant burden to the affected individuals and their families(Paulus and Stewart,2020).Neurotransmitter system aberrations,mitochondrial dysfunction,oxidative stress,and inflammasome activation have been involved in METH-induced neurotoxicity(Shin et al.,2018;Wang et al.,2015;Wei et al.,2020).

关键词： China Stewart

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