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Laser-based transfection with conjugated gold nanoparticles

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Chinese Optics Letters 2009年第10期7卷 898-900页

作者：姚翠萍屈晓超张镇西 Key Laboratory of biomedical Information Engineering of Ministry of Education and school of Life science and Technology Xi'an Jiaotong University Life science Research Center School of Electronic EngineeringXidian University

The irradiation of ceils combined with the immunoconjugate of gold nanoparticles by the short pulse laser can make the plasma membrane be transiently permeabilized, which can be used to transfer exogenous molecules into the cells. We explore this technique as a novel gene transfection method for floating cells. Three different floating cells exposed to the laser are selectively transfected with fluorescein isothiocyanatedextran, antibody, and green fluorescent protein （GFP） coding plasmids, and the viability of cells are determined by propidium iodide. For fluorescein isothiocyanate-dextran, the best transfection efficiency of 65% is obtained; for the antibody, it is 74%; whereas for the green fluorescent protein coding plasmids, a very small transfection efficiency is gained. If the transfection efficiency is improved, gold nanoparticles will be very useful as mediator for gene transfection in living cells.

关键词： Antibodies Cell membranes Dextran Fluorescence Glucose Gold Lasers Microsensors Nanoparticles Pulsed laser applications

Synthesis of noble metal-based intermetallic electrocatalysts by space-confined pyrolysis:Recent progress and future perspective

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Journal of Energy Chemistry 2021年第9期30卷 61-74页

作者： Lei Zhao Rui Wu Junjie Wang Zhao Li Xinxin Wei Jun Song Chen Yuan Chen school of Materials and Energy University of Electronic Science and Technology of ChinaChengdu 611731China Center for Applied Chemistry University of Electronic Science and Technology of ChinaChengdu 611731China school of Chemical and biomolecular Engineering The University of Sydney2006 SydneyAustralia

Noble metal-based intermetallics are promising electrocatalysts for sustainable energy conversion and consumption ***-temperature pyrolysis(>500°C)methods are used to control their crystalline orderings,critical to their electrocatalytic activity and ***,the high temperature would cause severe aggregation,resulting in a low catalytic active surface *** research efforts have been devoted to addressing this *** short review summarizes recent research progress on synthesizing noble metal-based intermetallic electrocatalysts by space-confined *** focus on three strategies:isolation in pores,coverture by shells,and immobilization by *** advantages and existing problems of different methods are ***,important issues to be addressed in future research are also *** hope that this article will stimulate future research to develop high-performance intermetallic catalysts for practical applications.

关键词： Intermetallic nanoparticles Annealing Space-confined approaches Particle size Electrocatalytic performance

Advances and applications in single-cell and spatial genomics

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science China Life sciences 2025年

作者： Jingjing Wang Fang Ye Haoxi Chai Yujia Jiang Teng Wang Xia Ran Qimin Xia Ziye Xu Yuting Fu Guodong Zhang Hanyu Wu Guoji Guo Hongshan Guo Yijun Ruan Yongcheng Wang Dong Xing Xun Xu Zemin Zhang Beijing Advanced Innovation Center for Genomics (ICG) Peking University Guangdong Provincial Key Laboratory of Genome Read and Write BGI Research Bone Marrow Transplantation Center of the First Affiliated Hospital & Liangzhu Laboratory Zhejiang University School of Medicine Life sciences Institute and The Second Affiliated Hospital Zhejiang University BGI Research biomedical Pioneering Innovation Center (BIOPIC) and school of Life sciences Peking University Academy for Advanced Interdisciplinary Studies Peking University Institute of Hematology Zhejiang University Department of Laboratory Medicine of The First Affiliated Hospital & Liangzhu Laboratory Zhejiang University School of Medicine Center for Stem Cell and Regenerative Medicine Zhejiang University School of Medicine Zhejiang Provincial Key Lab for Tissue Engineering and Regenerative Medicine Dr.Li Dak Sum & Yip Yio Chin Center for Stem Cell and Regenerative Medicine

The applications of single-cell and spatial technologies in recent times have revolutionized the present understanding of cellular states and the cellular heterogeneity inherent in complex biological systems. These advancements offer unprecedented resolution in the examination of the functional genomics of individual cells and their spatial context within tissues. In this review, we have comprehensively discussed the historical development and recent progress in the field of single-cell and spatial genomics. We have reviewed the breakthroughs in single-cell multi-omics technologies, spatial genomics methods, and the computational strategies employed toward the analyses of singlecell atlas data. Furthermore, we have highlighted the advances made in constructing cellular atlases and their clinical applications,particularly in the context of disease. Finally, we have discussed the emerging trends, challenges, and opportunities in this rapidly evolving field.

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Nitrogen-rich carbon spheres made by a continuous spraying process for high-performance supercapacitors

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Nano Research 2016年第11期9卷 3209-3221页

作者： Fei Sun Haobin Wu Xin Liu Fang Liu Huihui zhou Jihui Gao Yunfeng Lu school of Energy science and Engineering Harbin Institute of Technology Harbin 150001 China Department of Chemical and biomolecular Engineering University of California Los Angeles CA 90095 USA

Supercapacitors have high power densities, high efficiencies, and long cycling lifetimes; however, to enable their wider use, their energy densities must be significantly improved. The design and synthesis of improved carbon materials with better capacitance, rate performance, and cycling stability has emerged as the main theme of supercapacitor research. Herein, we report a facile synthetic method to prepare nitrogen-rich carbon particles based on a continuous aerosol- spraying process. The method yields particles that have high surface areas, a uniform microporous structure, and are highly N-doped, resulting in a synergism that enables the construction of supercapacitors with high energy and power density for use in both aqueous and commercial organic electrolytes. Furthermore, we have used density functional theory calculations to show that the improved performance is due to the enhanced wettability and ion adsorption interactions at the carbon/electrolyte interface that result from nitrogen doping. These findings provide new insights into the role of heteroatom doping in the capacitance enhancement of carbon materials; in addition, our method offers an efficient route for large-scale production of doped carbon.

关键词： nitrogen doping carbon spheres scalable supercapacitors density functional theory(DFT)

Two-photon imaging of lymphoma cells targeted by gold nanoparticles

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Chinese Optics Letters 2008年第12期6卷 879-881页

作者：屈晓超王晶姚翠萍张镇西 Life science Research Centerschool of Electronic Engineering Xidian University Key Laboratory of biomedical Information Engineering of Ministry of Education Institute of Biomedical Analytical Technology and InstrumentationSchool of Life Science and TechnologyXi'an Jiaotong University

Gold nanoparticles （NPs） have highly efficient multi-photon-induced luminescence. In this paper, we record the two-photon images of gold NPs, lymphoma cell line Karpas 299, and Karpas 299 incubated with 30-nm-diameter gold NPs and ACT-1 antibody conjugates （Au30-ACT-1 conjugates） by using a multi-photon microscopy system. Due to the specific conjugation of ACT-1 antibody and cell membrane receptor CD25, gold NPs are only bound to the surface of cell membrane of Karpas 299. The luminescence intensity of gold NPs is higher than that of cells at 750-nm laser excitation. By comparing the images of Karpas 299 cells incubated with and without gold NPs, it is found that by means of gold NPs, we can get clear cell images with lower excitation power. Their excellent optical and chemical properties make gold NPs an attractive contrast agent for cellular imaging.

关键词： Animal cell culture Cell culture Cell membranes Chemical properties Cytology Image enhancement Laser excitation Light emission Light sources Luminescence Nanoparticles Photons Scanning

FeCo alloy/N,S co-doped carbon aerogel derived from directionalcasting cellulose nanofibers for rechargeable liquid flow and flexible Zn–air batteries

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Nano Research 2023年第5期16卷 6870-6880页

作者： Yiwen Zhang Xifeng Zhang Yuting Li Juan Wang Sibudjing Kawi Qin Zhong school of Chemistry and Chemical Engineering Nanjing University of Science and TechnologyNanjing 210094China Department of Chemical and biomolecular Engineering National University of SingaporeSingapore 117582Singapore

Bifunctional oxygen reduction reaction(ORR)and oxygen evolution reaction(OER)electrocatalysts with the advantages of excellent activity and stability are the vital components of air cathodes for rechargeable Zn–air batteries(ZABs).Herein,the carbon aerogel with honeycomb-like structure,N and S double doping and loaded with FeCo alloy nanoparticles(NSCA/FeCo)was prepared successfully as cathodes for rechargeable liquid flow and two-dimensional flexible ZABs by clever directional *** interaction between the bimetallic alloy and the double-doped carbon with specifical structure,large surface,great conductivity endows NSCA/FeCo with effective ORR/OER active sites and small charge/mass transport barrier,thus achieving outstanding bifunctional catalytic *** NSCA/FeCo displays a half-wave potential of+0.85 V(*** hydrogen electrode(RHE))for ORR and an overpotential of 335 mV at a current density of 10 mA·cm^(−2)for OER,which is even comparable to the performance of noble-metal catalysts in relevant fields(Pt/C for ORR and RuO_(2)for OER).Consequently,the rechargeable liquid flow ZABs assembled with NSCA/FeCo showed excellent performance(maximum power density:132.0 mW·cm^(−2),specific capacity:804.5 Wh·kg^(−1)at 10 mA·cm^(−2),charge and discharge cycle stability of more than 250 cycles).Furthermore,the flexible NSCA/FeCo-based ZABs have a maximum power density of 43.0 mW·cm^(−2),outstanding charging–discharge stability of more than 450 cycles,exhibit good flexibility under different bending ***,this work has provided an efficient bifunctional electrocatalyst for OER/ORR and a promising strategy of air cathodes for rechargeable and wearable ZABs.

关键词： carbon aerogel bifunctional electrocatalyst FeCo alloy directional freeze-casting rechargeable zinc–air batteries flexible batteries

Gold nanocages as multifunctional materials for nanomedicine

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Frontiers of physics 2014年第3期9卷 378-384页

作者： Xiaohu Xia The Wallace H. Coulter Department of biomedical Engineering Georgia Institute of Technology and Emory University Medical School Atlanta GA 30332 USA school of Chemistry and Biochemistry School of Chemical and Biomolecular Engineering Georgia Institute of Technology Atlanta GA 30332 USA

Featured by tunable localized surface plasmon resonance peaks in the near-infrared region and hollow interiors, Au nanocages represent a novel class of multifunctional nanomat, erials that have gained considerable attention in recent vears. This short review summarizes our recent work on the capabilities of An nanocages in nanomedicine. We start with a brief description of the synthesis of Au nanocages and highlight our recent protocols for the scaled-up production of An ***. We then use a numer of examples to illustrate how Au nanocages can contribute to nanomedicine with respect to both diagnosis and therapy.

关键词： gold nanocage nanomedicine diagnosis therapy

Imaging the structure and organization of mouse cerebellum and brain stem with second harmonic generation microscopy

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Chinese Optics Letters 2017年第9期15卷 7-11页

作者： Xiuli Liu Daozhu Hua Ling Fu Shaoqun Zeng Britton Chance Center for biomedical Photonics School of Engineering Sciences Wuhan National Laboratory for Optoelectronics--Huazhong University of Science and Technology Wuhan 430074 China MoE Key Laboratory for biomedical Photonics Department of Biomedical Engineering Huazhong University of Science and Technology Wuhan 430074. China

To visualize the structure and organization of the brain is a fundamental requirement in the research of neuroscience. Here, combining with two-photon excitation fluorescence microscopy and transgenetic mouse GAD67,we demonstrate a custom-built second harmonic generation（SHG） microscope to discriminate brain layers and sub regions in the cerebellum and brain stem slices with cellular resolution. In particular, the cell densities of neurons in different brain layers are extracted due to the cell soma appearing as dark shadow on an SHG ***, the axon initial segments of the Purkinje cell are easily recognized without labeling, which would be useful for guiding micropipettes for electrophysiology.

关键词： SHG Imaging the structure and organization of mouse cerebellum and brain stem with second harmonic generation microscopy

Strategies for engineering advanced nanomedicines for gas therapy of cancer

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National science Review 2020年第9期7卷 1485-1512页

作者： Yingshuai Wang Tian Yang Qianjun He Guangdong Provincial Key Laboratory of biomedical Measurements and Ultrasound Imaging National-Regional Key Technology Engineering Laboratory for Medical UltrasoundSchool of Biomedical Engineering Health Science CenterShenzhen University

As an emerging and promising treatment method, gas therapy has attracted more and more attention for treatment of inflammation-related diseases, especially cancer. However, therapeutic/therapy-assisted gases（NO, CO, H2S, H2, O2, SO2and CO2） and most of their prodrugs lack the abilities of active intratumoral accumulation and controlled gas release, resulting in limited cancer therapy efficacy and potential side effects. Therefore, development of nanomedicines to realize tumor-targeted and controlled release of therapeutic/therapy-assisted gases is greatly desired, and also the combination of other therapeutic modes with gas therapy by multifunctional nanocarrier platforms can augment cancer therapy efficacy and also reduce their side effects. The design of nanomedicines with these functions is vitally important, but challenging. In this review, we summarize a series of engineering strategies for construction of advanced gas-releasing nanomedicines from four aspects:（1） stimuli-responsive strategies for controlled gas release;（2） catalytic strategies for controlled gas release;（3） tumor-targeted gas delivery strategies;（4）multi-model combination strategies based on gas therapy. Moreover, we highlight current issues and gaps in knowledge, and envisage current trends and future prospects of advanced nanomedicines for gas therapy of cancer. This review aims to inspire and guide the engineering of advanced gas-releasing nanomedicines.

关键词： nanomedicine gas therapy drug delivery controlled release cancer treatment