Imaging the structure and organization of mouse cerebellum and brain stem with second harmonic generation microscopy

作     者：Xiuli Liu Daozhu Hua Ling Fu Shaoqun Zeng 刘秀丽;华道柱;付玲;曾绍群

作者机构：Britton Chance Center for Biomedical Photonics School of Engineering Sciences Wuhan National Laboratory for Optoelectronics--Huazhong University of Science and Technology Wuhan 430074 China MoE Key Laboratory for Biomedical Photonics Department of Biomedical Engineering Huazhong University of Science and Technology Wuhan 430074. China 

出 版 物：《Chinese Optics Letters》 (中国光学快报（英文版）)

年 卷 期：2017年第15卷第9期

页      面：7-11页

核心收录：

学科分类：0710[理学-生物学] 07[理学] 071006[理学-神经生物学] 

基　　金：supported by the National Key Research and Development Program of China(No.2016YFA0201403) the National Natural Science Foundation of China(No.61522502) the Science Fund for Creative Research Group of China(No.61421064) 

主　　题：SHG Imaging the structure and organization of mouse cerebellum and brain stem with second harmonic generation microscopy 

摘      要：To visualize the structure and organization of the brain is a fundamental requirement in the research of neuroscience. Here, combining with two-photon excitation fluorescence microscopy and transgenetic mouse GAD67,we demonstrate a custom-built second harmonic generation（SHG） microscope to discriminate brain layers and sub regions in the cerebellum and brain stem slices with cellular resolution. In particular, the cell densities of neurons in different brain layers are extracted due to the cell soma appearing as dark shadow on an SHG ***, the axon initial segments of the Purkinje cell are easily recognized without labeling, which would be useful for guiding micropipettes for electrophysiology.