Laser-based transfection with conjugated gold nanoparticles

作     者：姚翠萍 屈晓超 张镇西 

作者机构：Key Laboratory of Biomedical Information Engineering of Ministry of Education and School of Life Science and TechnologyXi'an Jiaotong University Life Science Research CenterSchool of Electronic EngineeringXidian University 

出 版 物：《Chinese Optics Letters》 (中国光学快报（英文版）)

年 卷 期：2009年第7卷第10期

页      面：898-900页

核心收录：

学科分类：0831[工学-生物医学工程（可授工学、理学、医学学位）] 08[工学] 0836[工学-生物工程] 

基　　金：supported by the China Scholarship Council,the National Natural Science Foundation of China (Nos.60578026 and 60878056) the Natural Science Basic Research Project in Shaanxi Province,China (No.2009JQ4013.) 

主　　题：Antibodies Cell membranes Dextran Fluorescence Glucose Gold Lasers Microsensors Nanoparticles Pulsed laser applications 

摘      要：The irradiation of ceils combined with the immunoconjugate of gold nanoparticles by the short pulse laser can make the plasma membrane be transiently permeabilized, which can be used to transfer exogenous molecules into the cells. We explore this technique as a novel gene transfection method for floating cells. Three different floating cells exposed to the laser are selectively transfected with fluorescein isothiocyanatedextran, antibody, and green fluorescent protein （GFP） coding plasmids, and the viability of cells are determined by propidium iodide. For fluorescein isothiocyanate-dextran, the best transfection efficiency of 65% is obtained; for the antibody, it is 74%; whereas for the green fluorescent protein coding plasmids, a very small transfection efficiency is gained. If the transfection efficiency is improved, gold nanoparticles will be very useful as mediator for gene transfection in living cells.