检索结果-南通市图书馆

The cloning of non-structural-1 (NS1) gene of H9N2 subtype of avian influenza virus in pGEX-4T-1 and pMAL-c2X plasmids and expression in <i>Escherichia coli</i>DH5<i>α</i>strain

引用

Advances in Bioscience and Biotechnology 2012年第3期3卷 283-289页

作者： Ramin Soleimani Mehdi Vasfi Marandi Mohammad Bagher Hashemi-Soteh Farhid Hemmatzadeh Department of Poultry Diseases Faculty of Veterinary Medicine University of Tehran-Iran Tehran Iran Medical Faculty and Cellular and Molecular Research Center Mazandaran University of Medical Sciences Sari Iran School of Animal and Veterinary Sciences The University of Adelaide Adelaide Australia

Avian influenza is a viral contagious disease that affects poultry industry and human health. Vaccination has been considered as a preventive tool in the eradication of AI, but it causes some limitations including trade embargoes and interfering with serologic surveillance in differentiation between infected and vaccinated animals (DIVA strategy). Several distinct DIVA strategies have been presented to conquer these limitations. In this study, the open reading frame of NS1 gene of a H9N2 subtype of AI virus was amplified by polymerase chain reaction. After extraction and purification of NS1 gene from agarose gel, it was inserted into two different pGEX-4T-1 and pMAL-c2X plasmids and transferred in DH5α strain of Escherichia coli by using electroporation procedure. The E. coli colonies possessing recombinant NS1 gene were screened using PCR, restriction mapping and sequencing analysis. The expressed rNS1 protein was purified using affinity chromatography based on MBP (pMAL- c2X) and GST (pGEX-4T-1). The MBP-NS1 and GST- NS1 proteins on SDS-PAGE had bands with molecular weight of 68 and 52 kDa respectively. Western blotting with MBP-NS1 protein showed positive reaction using antisera obtained from chickens challenged with a H9N2 subtype strain. But, the most sera prepared from H9N2 vaccinated chickens were negative in WB. These findings indicated that the MBP-rNS1 protein of 26 kDa expressed by pMAL-c2X plasmid can be used in a DIVA for differentiation of AI infected and vaccinated chickens.

关键词： Avian Influenza Virus H9N2 Subtype Recombinant Protein Non-Structural Protein 1 DIVA

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Potential therapeutic applications for Terminalia chebula in Iranian traditional medicine

引用

Journal of Traditional Chinese Medicine 2016年第2期36卷 250-254页

作者： Assie Jokar Fatemeh Masoomi Omid Sadeghpour Mohsen Nassiri-Toosi Shokouhsadat Hamedi Iranian Traditional Medicine Faculty Tehran University of Medical SciencesTehran ***Iran Department of Iranian Traditional Medicine Mazandaran University of Medical SciencesSari ***Iran Department of Traditional Pharmacy Traditional Medicine FacultyTehran University of Medical SciencesTehran ***Iran Herbal and Traditional Medicine Department Research Institute for Islamic and Complementary MedicineIran University of Medical SciencesTehran ***Iran Department of Internal Medicine Tehran University of Medical SciencesTehran ***Iran Department of Persian Pharmacy School of Persian and Complementary MedicineMashhad University of Medical SciencesMashhad ***Iran

Terminalia chebula(family:Combretaceae)is widely used in the traditional medicine of India and Iran to treat diseases that include dementia,constipation,and *** tree is known in Iranian traditional medicine(ITM)as halileh or halilaj and the fruit is used to develop *** is described in ITM as an astringent that has a"cold"and"dry"*** to the medicinal properties of Terminalia chebula were collected from important ITM sources and from modern medical databases(Pub Med,Scirus,Science Direct,and Scopus).The medicinal properties described for thistree in ITM were compared with those reported in studies of modern *** results confirm that the tree referred to as halileh in traditional books is the Terminalia chebula used in present-day *** that have not been evaluated in modern phytotherapy but have been traditionally treated with Terminalia chebula include fever,and psychological and psychiatric *** article confirms the medicinal uses of Terminalia chebula.

关键词： Fructus chebula Phytochemicals Phar macology Iranian traditional medicine

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Evaluation of the effect of genetic diversity and phylogenetic relationships on different strains of Staphylococcus aureus in clinical specimens

引用

Microenvironment & Microecology Research 2021年第4期3卷 1-3页

作者： Dariush D.Farhud Ali Ahmadi Prasa Saberian Sina Kor Farhud Genetic Clinic TehranIran Department of Biological Sciences and Technologies Islamic Azad University Sari BranchSariIran Department of Medical Sciences Hormozgan University of Medical SciencesHormozganIran Department of Medical Sciences Islamic Azad UniversitySari BranchSariIran

Introduction:Staphylococcus aureus is one of the main pathogens in ***,this bacterium can be colonized in the *** spa gene is present in all strains of Staphylococcus aureus(***)and can be used to identify species and toxins such as Panton-Valentine Leukocidin(PVL)that can affect a wide range of skin,soft tissue,and necrotizing *** and life-threatening *** study aimed to investigate the effect of genetic diversity and phylogenetic relationships on different strains of Staphylococcus aureus in clinical ***:This review study was conducted in 2021 by searching for keywords such as genetic diversity,phylogenetics,and Staphylococcus aureus in reputable databases such as pub med and google ***,30 articles were found,of which 30 articles were used out of 26 ***:In general,23%and 18%of health care workers and patients were carriers,*** incidence of methicillin-resistant Staphylococcus aureus(MRSA)is higher in the relevant *** molecular types of MRSA isolates indicate that the most common virulence gene is Hla,and the most common type of SCCmec is SCCmec type ***:Sites with high risk of Staphylococcus aureus should be highly protected,and relevant personnel should be paid attention and health management.

关键词： Staphylococcus aureus Resistance Genetic diversity Phylogenetic relationships

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Errata for the article“Application of Adomian decomposition method for micropolar flow in a porous channel”

引用

Propulsion and Power Research 2021年第3期10卷 314-315页

作者： F.Shakeri Aski Seyed Jalal Nasirkhani E.Mohammadian A.Asgari Department of Mechanical Engineering Islamic Azad UniversityNajafabad BranchIsfahanIran Department of Mechanical Engineering Shahid Bahonar University of KermanKermanIran Department of Mechanical Engineering Technical&Professional University-Imam Mohamadbagher Technical CollegeSariIran Department of Mechanical Engineering Mechanics GroupRamsar BranchIslamic Azad UniversityRamsarIran Department of Manufacturing Engineering School of Mechanical EngineeringIran University of Science and TechnologyNarmakTehranIran

In this paper,the errata for a published paper,titled“Application of Adomian decomposition method for micropolar flow in a porous channel,authored by *** Aski,Seyed Jalal Nasirkhani,***,***,in the journal of Propulsion and Power Research 3(1)(2014)15-21”,were *** micropolar flow in a porous channel attracts considerable research interest,it is necessary to correct the minor typo errors in the boundary conditions of the *** should be said that the typo errors have not affected the results because the correct boundary conditions have been applied in the written maple *** is just a typo error in the written boundary conditions in the paper.

关键词： porous flow polar

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Mortality Rate and Outcome among Patients Admitted to General Intensive Care Unit during “Morning-Hour” Compared with “Off-Hour”

引用

International Journal of Clinical Medicine 2012年第3期3卷 171-177页

作者： Shahram Ala Nasrin Pakravan Motahhare Ahmadi 1Imam Khomeini Teaching Hospital Mazandaran University of Medical Science Sari Iran 2Faculty of Pharmacy Mazandaran University of Medical Science Faculty of Pharmacy Mazandaran University of Medical Science Faculty of Pharmacy Mazandaran University of Medical Science.

Aim: This study aim to evaluate the effect of time of admission on mortality of patients admitted to the ICU. Method: This retrospective study included 391 of patients admitted to the ICU of an academic hospital during one year. Patients were categorized according to time of admission: working-hours and off-hours. Mortality was compared in the groups and associated factors of mortality were examined. Results: Two third of patients were admitted during off-hours. There was no significant difference in the GCS, age and length of stay among patients admitted during working-hours and off-hours. There was no difference in mortality among patients admitted during working-hours and off-hours Mortality was significantly higher in older and more severe cases, regardless of time of admission. Conclusion: Time of ICU admission does not have significant effect on patient’s outcome.

关键词： Critical Care Intensive Care Unit ICU Mortality

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Response to Abadi and Kusters, World J Gastroenterol 19: 429-430

引用

World Journal of Gastroenterology 2013年第23期19卷 3711-3712页

作者： Alireza Rafiei Jeremy J Gilbreath D Scott Merrell Molecular and Cell Biology Research Center Mazandaran University of Medical SciencesSari 48471-91971 Iran Department of Microbiology and Immunology Uniformed Services University of the Health SciencesBethesda MD 20814 United States

In a recent study, Rafiei et al, reported a link between a C150T polymorphism in the human inducible nitric oxide gene and Helicobacter pylori infection as a risk factor for gastric cancer among an Iranian population. Subsequently, Abadi and Kusters published a letter to the editor questioning the validity of the study because of a supposed flaw in primer design. Here we respond to the claims of Abadi and Kusters and show that the results reported in the original article are valid.

关键词： Inducible nitric oxide synthetase Helicobacter pylori Gastric cancer

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Measurement of pesticides in surface water and sediment of Caspian Sea, Iran

引用

Chinese Journal Of Geochemistry 2006年第B8期25卷 91-91页

作者： Changiz Mohammadizadeh Abdol Ghaffar Ebadi Geology Islamic Azad University Sari branch/Sari Iran Biology Islamic Azad University Jouybar Branch/Jouybar Iran

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Diabetes and skin cancers: Risk factors, molecular mechanisms and impact on prognosis

引用

World Journal of Clinical Cases 2022年第31期10卷 11214-11225页

作者： Elena-Codruta Dobrică Madalina Laura Banciu Vincent Kipkorir Mohammad Amin Khazeei Tabari Madeleine Jemima Cox L V Simhachalam Kutikuppala Mihnea-Alexandru Găman Doctoral School University of Medicine and Pharmacy of CraiovaCraiova 200349Romania Department of Dermatology "Elias"University Emergency HospitalBucharest 011461Romania Department of Human Anatomy University of NairobiCollege of Health SciencesNairobi 00100Kenya USERN Office Mazandaran University of Medical SciencesSari 48175-866Iran University of New South Wales University of New South WalesSydney 2052Australia Dr NTR University of Health Sciences VijayawadaAndhra Pradesh 520008India Faculty of Medicine "Carol Davila"University of Medicine and PharmacyBucharest 050474Romania

Diabetes and skin cancers have emerged as threats to public health ***,their association has been less intensively *** this narrative review,we explore the common risk factors,molecular mechanisms,and prognosis of the association between cutaneous malignancies and ***,oxidative stress,low-grade chronic inflammation,genetic,lifestyle,and environmental factors partially explain the crosstalk between skin cancers and this metabolic *** addition,diabetes and its related complications may interfere with the appropriate management of cutaneous *** medication seems to exert an antineoplastic effect,however,future large,observation studies with a prospective design are needed to clarify its impact on the risk of malignancy in *** for diabetes in skin cancers,as well as close follow-up for the development of cutaneous malignancies in subjects suffering from diabetes,is warranted.

关键词： Diabetes Skin cancers Squamous cell carcinoma Melanoma Basocellular carcicoma

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Distribution and associated factors of intraocular pressure in the older population:Tehran Geriatric Eye Study

引用

International Journal of Ophthalmology(English edition) 2023年第3期16卷 418-426页

作者： Hassan Hashemi Samira Heydarian Mohammadreza Aghamirsalim Abbasali Yekta Alireza Hashemi Masoumeh Sajadi Mehdi Khabazkhoob Noor Research Center for Ophthalmic Epidemiology Noor Eye HospitalTehran 19839-63113Iran Department of Rehabilitation Sciences School of Allied Medical SciencesMazandaran University of Medical SciencesSari ***Iran Translational Ophthalmology Research Center Tehran University of Medical SciencesTehran ***Iran Department of Optometry School of Paramedical SciencesMashhad University of Medical SciencesMashhad 3453545Iran Noor Ophthalmology Research Center Noor Eye HospitalTehran ***Iran Department of Basic Sciences School of Nursing and MidwiferyShahid Beheshti University of Medical SciencesTehran ***Iran

AIM:To determine the distribution and associated factors of intraocular pressure(IOP) in an Iranian elderly population 60 years of age and ***:The present report is part of the Tehran Geriatric Eye study(TGES),a population-based cross-sectional study that was conducted on the residents of Tehran 60 years of age and *** sampling was performed using multistage stratified random cluster sampling methods from 22 districts of Tehran,*** and history information,blood samples,and blood pressure were collected from all *** examinations included measurement of uncorrected and best-corrected visual acuity,objective and subjective refraction,and slit-lamp *** IOP was measured using Goldmann applanation tonometry(GAT).Corneal imaging and ocular biometry were performed using Pentacam ***:The data of 3892 eyes of 2124 individuals were analyzed for this *** mean age of the study participants was 66.49±5.31y(range:60 to 95y).The mean IOP was 15.2 mm Hg(95%CI:15.1 to 15.4),15.3 mm Hg(95%CI:15.1 to 15.5) and 15.1 mm Hg(95%CI:15.0 to 15.3) in all participants,males,and females,*** the study participants,1.3% had an IOP of ≥20 mm *** mean IOP increased from 15.1 mm Hg in the age group 60-64y to 16.3 mm Hg in the age group ≥*** to the final multiple GEE model,the IOP was statistically significantly higher in men than in *** the studied age groups,except for the 75-79-year-old age group,had significantly higher IOP compared to the 60-64-year-old age *** IOP was significantly higher in underweight compared to other body mass index ***,the IOP had a statistically significant direct relationship with the mean corneal power(mean CP),central corneal thickness(CCT),and systolic blood ***:The present study presents the distribution of IOP in an Iranian elderly population.A higher IOP(within the range 14 to 17 mm Hg) is significantly associated with older age

关键词： intraocular pressure glaucoma elderly ocular biometry

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Epidemiological aspect of retinoblastoma in the world: a review of recent advance studies

引用

International Journal of Ophthalmology(English edition) 2023年第6期16卷 962-968页

作者： Leili Koochakzadeh Abbasali Yekta Hassan Hashemi Reza Pakzad Samira Heydarian Mehdi Khabazkhoob Department of Pediatrics Children’s Medical Center HospitalTehran University of Medical SciencesTehran ***Iran Refractive Errors Research Center Mashhad University of Medical SciencesMashhad 3453545Iran Noor Research Center for Ophthalmic Epidemiology Noor Eye HospitalTehran ***Iran Department of Epidemiology Faculty of HealthIlam University of Medical SciencesIlam ***Iran Department of Rehabilitation Science School of Allied Medical SciencesMazandaran University of Medical SciencesSari ***Iran Department of Basic Sciences School of Nursing and MidwiferyShahid Beheshti University of Medical SciencesTehran ***Iran

·AIM: To collect and present updated evidence about epidemiological aspects of retinoblastoma(Rb) in the world.·METHODS: A comprehensive search without the time and language restrictions was conducted in international databases, including MEDLINE, Scopus, Web of Science, and Pub Med. The search keywords were “retinoblastoma” OR “retinal Neuroblastoma” OR “retinal glioma” OR “retinoblastoma eye cancer” OR “retinal glioblastoma”.·RESULTS: The worldwide incidence of Rb is 1 in 16 000-28 000 live births, but was higher in developing compared to developed countries. Several attempts for improving early detection and treatment had increased the Rb survival rate from 5% to 90% in developed countries over the past decade, but its survival was lower in developing countries(about 40% in low-income countries) and the majority of mortalities occurred in developing countries. The etiology of Rb could be viewed as genetics in the heritable form and environmental and lifestyle factors in the sporadic form. Some environmental risk factors such as in vitro fertilization;insect sprays;father’s occupational exposure to oil mists in metal working, and poor living conditions might play a role in the occurrence of the disease. Although ethnicity might affect Rb incidence, sex has no documented effect and the best treatment approaches were now ophthalmic artery chemosurgery and intravitreal chemotherapy.·CONCLUSION: Determining the role of genetics and environmental factors helps to accurately predict the prognosis and identify the mechanism of the disease, which can reduce the risk of tumor development.

关键词： epidemiology retinoblastoma leukocoria children RB1 gene

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：