检索结果-南通市图书馆

Application of Nanopore Sequencing Technology in the Clinical Diagnosis of Infectious Diseases

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Biomedical and Environmental Sciences 2022年第5期35卷 381-392页

作者： ZHANG Lu Lu ZHANG Chi PENG Jun Ping NHC Key laboratory of Systems biology of Pathogens Institute of Pathogen BiologyChinese Academy of Medical Sciences&Peking Union Medical CollegeBeijing 100176China Key laboratory of Respiratory Disease Pathogenomics Chinese Academy of Medical SciencesBeijing 100176China

Infectious diseases are an enormous public health burden and a growing threat to human health *** or classic recurrent pathogens,or pathogens with resistant traits,challenge our ability to diagnose and control infectious *** sequencing technology has the potential to enhance our ability to diagnose,interrogate,and track infectious diseases due to the unrestricted read length and system *** review focuses on the application of nanopore sequencing technology in the clinical diagnosis of infectious diseases and includes the following:(i)a brief introduction to nanopore sequencing technology and Oxford Nanopore Technologies(ONT)sequencing platforms;(ii)strategies for nanopore-based sequencing technologies;and(iii)applications of nanopore sequencing technology in monitoring emerging pathogenic microorganisms,molecular detection of clinically relevant drug-resistance genes,and characterization of disease-related microbial ***,we discuss the current challenges,potential opportunities,and future outlook for applying nanopore sequencing technology in the diagnosis of infectious diseases.

关键词： Nanopore sequencing Infectious diseases Pathogen Oxford Nanopore Technologies

Oligodendroglial heterogeneity in health,disease,and recovery:deeper insights into myelin dynamics

在线全文

学校读者我要写书评

暂无评论

Neural Regeneration Research 2025年第11期20卷 3179-3192页

作者： Pieter-Jan Serneels Julie D.De Schutter Lies De Groef Lieve Moons Steven Bergmans KU Leuven Leuven Brain InstituteDepartment of BiologyAnimal Physiology and Neurobiology DivisionNeural Circuit Development&Regeneration Research GroupLeuvenBelgium KU Leuven Leuven Brain InstituteDepartment of BiologyAnimal Physiology and Neurobiology DivisionCellular Communication&Neurodegeneration Research GroupLeuvenBelgium

Decades of research asserted that the oligodendroglial lineage comprises two cell types:oligodendrocyte precursor cells and ***,recent studies employing single-cell RNA sequencing techniques have uncovered novel cell states,prompting a revision of the existing *** forward,the oligodendroglial lineage should be delineated into five distinct cell states:oligodendrocyte precursor cells,committed oligodendrocyte precursor cells,newly formed oligodendrocytes,myelin-forming oligodendrocytes,and mature *** new classification system enables a deeper understanding of the oligodendroglia in both physiological and pathological *** this uniform terminology will facilitate comparison and integration of data across ***,including the consolidation of findings from various demyelinating models,is essential to better understand the pathogenesis of demyelinating ***,comparing injury models across species with varying regenerative capacities can provide insights that may lead to new therapeutic strategies to overcome remyelination ***,by standardizing terminology and synthesizing data from diverse studies across different animal models,we can enhance our understanding of myelin pathology in central nervous system disorders such as multiple sclerosis,Alzheimer's disease,and amyotrophic lateral sclerosis,all of which involve oligodendroglial and myelin dysfunction.

关键词： animal models demyelination fish mammals multiple sclerosis myelin neurodegeneration oligodendrocytes oligodendroglial lineage remyelination

Malic enzyme 2 as a therapeutic target for cancer: comments on ‘Malic enzyme 2 maintains protein stability of mutant p53 through 2-hydroxyglutarate’

在线全文

学校读者我要写书评

暂无评论

Journal of Molecular Cell biology 2022年第4期14卷 72-73页

作者： Zhenxi Zhang Wenjing Du State Key laboratory of medical Molecular biology Department of Cell BiologyInstitute of Basic Medical Sciences Chinese Academy of Medical SciencesSchool of Basic Medicine Peking Union Medical CollegeBeijing 100005China

In mammalian cells,there are three isoformsof malicenzymes(MEs):a cytosolic nicotinamide adenine dinucleotide phosphate*(NADP+)dependent isoform(ME1),a mitochondrial NADP+-dependent isoform(ME2),and a mitochondrial NADp+-dependent isoform(ME3),of which ME1 and ME2 are the major *** essential modulators of metabolism and aging,MEs havebeen reported to be targetsfor wild-type p53(wtp53),which can transcriptionally repress their ***,downregulation of ME1 and ME2leads to wtp53activation via distinct mechanisms(iang et al.,2013;Figure 1A).

关键词： expression. metabolism therapeutic

Quantitative proteomic analysis for high-throughput screening of differential glycoproteins in hepatocellular carcinoma serum

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Cancer biology & Medicine 2015年第3期12卷 246-254页

作者： Hua-Jun Gao Ya-Jing Chen Duo Zuo Ming-Ming Xiao Ying Li Hua Guo Ning Zhang Rui-Bing Chen Research Center of Basic medical Sciences & School of medical laboratory Tianjin Medical University laboratory of Cancer Cell biology Tianjin Medical University Cancer Institute and Hospital National Clinical Research Center for Cancer Tianjin Key Laboratory of Cancer Prevention and Therapy

Objective： Hepatocellular carcinoma （HCC） is a leading cause of cancer-related deaths. Novel serum biomarkers are required to increase the sensitivity and specificity of serum screening for early HCC diagnosis. This study employed a quantitative proteomic strategy to analyze the differential expression of serum glycoproteins between HCC and normal control serum samples. Methods： Lectin affinity chromatography （LAC） was used to enrich glycoproteins from the serum samples. Quantitative mass spectrometric analysis combined with stable isotope dimethyl labeling and 2D liquid chromatography （LC） separations were performed to examine the differential levels of the detected proteins between HCC and control serum samples. Western blot was used to analyze the differential expression levels of the three serum proteins. Results： A total of 2,280 protein groups were identified in the serum samples from HCC patients by using the 2D LC-MS/MS method. Up to 36 proteins were up-regulated in the HCC serum, whereas 19 proteins were down-regulated. Three differential glycoproteins, namely, fibrinogen gamma chain （FGG）, FOS-like antigen 2 （FOSL2）, and a-l, 6-mannosylglycoprotein 6-^-N-acetylglucosaminyltransferase B （MGATSB） were validated by Western blot. All these three proteins were up-regulated in the HCC serum samples. Conclusion： A quantitative glycoproteomic method was established and proven useful to determine potential novel biomarkers for HCC.

关键词： Glycoprotein hepatocellular carcinoma (HCC) mass spectrometry proteomics serum

Diversity and independent evolutionary profiling of rodent-borne viruses in Hainan,a tropical island of China

在线全文

学校读者我要写书评

暂无评论

Virologica Sinica 2023年第5期38卷 651-662页

作者： Youyou Li Chuanning Tang Yun Zhang Zihan Li Gaoyu Wang Ruoyan Peng Yi Huang Xiaoyuan Hu Henan Xin Boxuan Feng Xuefang Cao Yongpeng He Tonglei Guo Yijun He Haoxiang Su Xiuji Cui Lina Niu Zhiqiang Wu Jian Yang Fan Yang Gang Lu Lei Gao Qi Jin Meifang Xiao Feifei Yin Jiang Du NHC Key laboratory of Systems biology of Pathogens Institute of Pathogen BiologyChinese Academy of Medical Sciences&Peking Union Medical CollegeBeijing100005China Department of Clinical laboratory Center for Laboratory MedicineHainan Women and Children's Medical CenterHaikou571199China Key laboratory of Tropical Translational Medicine of Ministry of Education Hainan Medical UniversityHaikou571199China Hainan medical University-The University of Hong Kong Joint laboratory of Tropical Infectious Diseases Hainan Medical UniversityHaikou571199China Department of Pathogen biology Hainan Medical UniversityHaikou571199China

The risk of emerging infectious diseases(EID)is increasing *** than 60%of EIDs worldwide are caused by animal-borne *** study aimed to characterize the virome,analyze the phylogenetic evolution,and determine the diversity of rodent-borne viruses in Hainan Province,*** collected 682 anal and throat samples from rodents,combined them into 28 pools according to their species and location,and processed them for next-generation sequencing and bioinformatics *** diverse viral contigs closely related to mammals were assigned to 22 viral *** clues of the important rodent-borne viruses were further identified by polymerase chain reaction for phylogenetic analysis and annotation of genetic characteristics such as arenavirus,coronavirus,astrovirus,pestivirus,parvovirus,and *** identified pestivirus and bocavirus in Leopoldoms edwardsi from Huangjinjiaoling,and bocavirus in Rattus andamanensis from the national nature reserves of Bangxi with low amino acid identity to known pathogens are proposed as the novel species,and their rodent hosts have not been previously reported to carry these *** results expand our knowledge of viral classification and host range and suggest that there are highly diverse,undiscovered viruses that have evolved independently in their unique wildlife hosts in inaccessible areas.

关键词： Rodents Virome Phylogenetic analysis Novel viruses Wildlife

Advances in innate immune signaling:new activators and regulators

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

National Science Review 2016年第2期3卷 160-162页

作者： Juan Liu Xuetao Cao National Key laboratory of medical Immunology & Institute of Immunology Second Military Medical University National Key laboratory of medical Molecular biology & Department of Immunology Institute of Basic Medical SciencesChinese Academy of Medical Sciences

The innate immune system can detect the invading microbe components via three major pattern recognition receptorsToll-like receptors,RIG-I-like receptors and NOD-like receptors as well as a variety of cytoplasmic DNA or RNA *** innate receptors induce a series of intracellular downstream signaling pathways,leading to the efficient innate immune responses to eliminate pathogens(Fig.1).Meanwhile,

关键词： Advances in innate immune signaling IKK DNA TLR

Omics Approaches for Exploring Pneumoconiosis:A Review

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Biomedical and Environmental Sciences 2021年第1期34卷 71-82页

作者： LUO Ya QI Xian Mei PANG Jun Ling WANG Jing WANG Chen State Key laboratory of medical Molecular biology Institute of Basic Medical SciencesChinese Academy of Medical SciencesDepartment of PathophysiologyPeking Union Medical CollegeBeijing 100730China State Key laboratory of medical Molecular biology Institute of Basic Medical SciencesChinese Academy of Medical SciencesDepartment of PhysiologyPeking Union Medical CollegeBeijing 100730China

Pneumoconiosis,a fatal lung disease caused by dust inhalation and deposition,is one of the leading occupational diseases worldwide[1].The dust is primarily inorganic particles,such as silica particles,coal dust,and asbestos *** the diameter of dust is less than 5μm,it is defined as‘respirable dust’that can easily reach the distal airways and alveoli[2].

关键词： lung diseases occupational

IFN-inducible p47 GTPases display differential responses to Schistosoma japonicum acute infection

在线全文

学校读者我要写书评

暂无评论

Cellular & Molecular Immunology 2010年第1期7卷 69-76页

作者： Xuesong Chen Xiaotang Du Meijuan Zhang Donghui Zhang Minjun Ji Guanling Wu Department of Pathogen biology and Immunology Nanjing Medical UniversityNanjingChina Jiangsu Province Key laboratory of Modern Pathogen biology NanjingChina

Interferon gamma induced GTPase(IGTP)(also named lrgm3)and interferon gamma inducible protein 47(IRG-47)(also named lIrgd)are interferon(IFN)-inducible p47 GTPases that have been shown to regulate host resistance to intracellular *** knowledge has been known about the role of p47 GTPases in host responses against extracellular *** investigate possible roles of IGTP and IRG-47 in the course of Schistosoma japonicum infection,IGTP and IRG-47 knockout and wild-type(WT)mice were challenged with cercariae of ***,and host responses were *** the acute stage of *** infection,mice that lacked IGTP displayed similar parasite burden and pathological damage to WT ***,***-infected lRG-47-deficient mice,in contrast to IGTP-deficient mice and WT mice,showed significantly reduced worms and lower egg-burden,but intense granulomatous reaction evoked by schistosome eggs in peripheral parts of liver *** addition,upregulation of inflammation-related gene expression was observed in the spleen of IRG-47-deficient mice using oligonucleotide microarrays,in which multiple pathways of cytokine-cytokine receptor interaction,T-cell receptor signaling,complement,coagulation cascades and cell adhesion molecules were *** together,these data suggest that IGTP and IRG-47 might have distinct features that were differentially required for resistance to ***.

关键词： acute infection immunity p47 GTPase Schistosoma japonicum

Transcriptional Regulation of Urokinase Receptor in High-(95D) and Low-metastatic (95C) Human Lung Cancer Cells

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Acta Biochimica et Biophysica Sinica 2004年第6期36卷 405-411页

作者： Chun-Yang LI, Li TAN, Guo-Jing ZHANG, Ping LI, Chang TONG, Jing FAN, and Yun-Song ZHU* Key laboratory, the Education Ministry, Department of Biochemistry and Molecular biology, Shanghai medical College,Fudan University, Shanghai 200032, China Key laboratory the Education Ministry Department of Biochemistry and Molecular Biology Shanghai Medical College Fudan UniversityShanghai 200032 China

To study the transcriptional regulation of urokinase receptor (uPAR) in high- (95D) and low-metastatic (95C) human lung cancer cells, we performed PCR to amplify 2238 bp uPAR promoter from 95C and 95D cells. According to the results of sequencing, five different bases are found in uPAR promoter between 95C and 95D cells. The results of luciferase activity assay showed that these differences have no significant effect on the uPAR promoter activity. Based on a normal uPAR promoter, progressive truncated mutants were constructed. The transient transfection/reporter assay showed that the promoter region from –136 to +9 may interact with relevant nuclear factors, which result in different levels of uPAR expression between 95C and 95D cells.

关键词： uPAR promoter transcriptional regulation