Quantitative proteomic analysis for high-throughput screening of differential glycoproteins in hepatocellular carcinoma serum

作     者：Hua-Jun Gao Ya-Jing Chen Duo Zuo Ming-Ming Xiao Ying Li Hua Guo Ning Zhang Rui-Bing Chen 

作者机构：Research Center of Basic Medical Sciences & School of Medical Laboratory Tianjin Medical University Laboratory of Cancer Cell Biology Tianjin Medical University Cancer Institute and Hospital National Clinical Research Center for Cancer Tianjin Key Laboratory of Cancer Prevention and Therapy 

出 版 物：《Cancer Biology & Medicine》 (癌症生物学与医学（英文版）)

年 卷 期：2015年第12卷第3期

页      面：246-254页

核心收录：

学科分类：1002[医学-临床医学] 100214[医学-肿瘤学] 10[医学] 

基　　金：supported by grants from the National Natural Science Foundation of China(Grant No.21205088) 973 Project(Grant No.2011CB933100) National Science Fund for Distinguished Young Scholars(Grant No.81125019) Doctoral Research Fund from the Ministry of Education of China(Grant No.20121202120001) sponsored by the Scientific Research Foundation for the Returned Overseas Chinese Scholars,State Education Ministry 

主　　题：Glycoprotein hepatocellular carcinoma (HCC) mass spectrometry proteomics serum 

摘      要：Objective： Hepatocellular carcinoma （HCC） is a leading cause of cancer-related deaths. Novel serum biomarkers are required to increase the sensitivity and specificity of serum screening for early HCC diagnosis. This study employed a quantitative proteomic strategy to analyze the differential expression of serum glycoproteins between HCC and normal control serum samples. Methods： Lectin affinity chromatography （LAC） was used to enrich glycoproteins from the serum samples. Quantitative mass spectrometric analysis combined with stable isotope dimethyl labeling and 2D liquid chromatography （LC） separations were performed to examine the differential levels of the detected proteins between HCC and control serum samples. Western blot was used to analyze the differential expression levels of the three serum proteins. Results： A total of 2,280 protein groups were identified in the serum samples from HCC patients by using the 2D LC-MS/MS method. Up to 36 proteins were up-regulated in the HCC serum, whereas 19 proteins were down-regulated. Three differential glycoproteins, namely, fibrinogen gamma chain （FGG）, FOS-like antigen 2 （FOSL2）, and a-l, 6-mannosylglycoprotein 6-^-N-acetylglucosaminyltransferase B （MGATSB） were validated by Western blot. All these three proteins were up-regulated in the HCC serum samples. Conclusion： A quantitative glycoproteomic method was established and proven useful to determine potential novel biomarkers for HCC.