Application of Nanopore Sequencing Technology in the Clinical Diagnosis of Infectious Diseases

作     者：ZHANG Lu Lu ZHANG Chi PENG Jun Ping ZHANG Lu Lu;ZHANG Chi;PENG Jun Ping

作者机构：NHC Key Laboratory of Systems Biology of PathogensInstitute of Pathogen BiologyChinese Academy of Medical Sciences&Peking Union Medical CollegeBeijing 100176China Key Laboratory of Respiratory Disease PathogenomicsChinese Academy of Medical SciencesBeijing 100176China 

出 版 物：《Biomedical and Environmental Sciences》 (生物医学与环境科学（英文版）)

年 卷 期：2022年第35卷第5期

页      面：381-392页

核心收录：

学科分类：100208[医学-临床检验诊断学] 1004[医学-公共卫生与预防医学(可授医学、理学学位)] 1002[医学-临床医学] 100401[医学-流行病与卫生统计学] 10[医学] 

基　　金：supported by CAMS Innovation Fund for Medical Sciences (CIFMS)[2021-I2M-1-038] 

主　　题：Nanopore sequencing Infectious diseases Pathogen Oxford Nanopore Technologies 

摘      要：Infectious diseases are an enormous public health burden and a growing threat to human health *** or classic recurrent pathogens,or pathogens with resistant traits,challenge our ability to diagnose and control infectious *** sequencing technology has the potential to enhance our ability to diagnose,interrogate,and track infectious diseases due to the unrestricted read length and system *** review focuses on the application of nanopore sequencing technology in the clinical diagnosis of infectious diseases and includes the following:(i)a brief introduction to nanopore sequencing technology and Oxford Nanopore Technologies(ONT)sequencing platforms;(ii)strategies for nanopore-based sequencing technologies;and(iii)applications of nanopore sequencing technology in monitoring emerging pathogenic microorganisms,molecular detection of clinically relevant drug-resistance genes,and characterization of disease-related microbial ***,we discuss the current challenges,potential opportunities,and future outlook for applying nanopore sequencing technology in the diagnosis of infectious diseases.